EDTA and NTA Effectively Tune the Mineralization of Calcium Phosphate from Bulk Aqueous Solution

Abstract

This study describes the effects of nitrilotriacetic acid (NTA) and ethylenediaminotetraacetic acid (EDTA) on the mineralization of calcium phosphate from bulk aqueous solution. Mineralization was performed between pH 6 and 9 and with NTA or EDTA concentrations of 0, 5, 10, and 15 mM. X-ray diffraction and infrared spectroscopy show that at low pH, mainly brushite precipitates and at higher pH, mostly hydroxyapatite forms. Both additives alter the morphology of the precipitates. Without additive, brushite precipitates as large plates. With NTA, the morphology changes to an unusual rod-like shape. With EDTA, the edges of the particles are rounded and disk-like particles form. Conductivity and pH measurements suggest that the final products form through several intermediate steps.

The Potential of Calcium/Phosphate Containing MAO Implanted in Bone Tissue Regeneration and Biological Characteristics

“…Calcium phosphate had very low solubility in water [ 33 ] because the individual calcium cations and phosphate anions were tightly bonded to each other in an aqueous medium. However, by the aid of acid addition such as EDTA, solubility of calcium phosphate was increased in aqueous solution [ 34 , 35 , 36 , 37 ]. Then, the pores size and pores number decreased with increasing the concentration of Ca-P containing MAO treated samples.…”

Development of a thermodynamic approach to assist the control of the precipitation of hydroxyapatites and associated calcium phosphates in open systems

“…It is rationalized how setting these experimental parameters can help control the HAp morphology in the absence of organic solvent or additive. [33][34][35] From an industrial point of view, the establishment of such interconnection between the experimental parameters and the properties of the synthesized materials is of the utmost interest. [36][37][38] The purpose of this study lies in the indepth analysis of the thermodynamic aspects of the competitive precipitation of CaPs, whereas the complementary kinetic aspects will be investigated in a forthcoming article.…”

Matrix-Directed Mineralization for Bulk Structural Materials

“…It has been reported that, by adding the soluble proteins extracted from the prismatic layer (calcite) or the nacreous layer (aragonite) of red abalone shell into the mineralization system, the polymorph of the precipitated CaCO 3 can switch from calcite to aragonite back and forth . The mineral morphologies can be changed via the kinetic stabilization of soluble additives to specific crystal phases. − The bioinspired pathways developed to precisely control mineralization have been summarized in previous reviews. ,,,− However, as the precursory phases, which have been widely found in biomineralization process, are promising in the fast and scalable fabrication of MBSMs, it is worth detailed discussions of their potentials in making MBSMs.…”