Editorial: Proteomics of Microbial Human Pathogens

Soares, Nelson C.; Bou, Germán; Blackburn, Jonathan M.

doi:10.3389/fmicb.2016.01742

Cited by 10 publications

(7 citation statements)

References 4 publications

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“…TB burden is especially dramatic in sub-Saharan countries 52 ; Mozambique, as the country of origin in the second cohort is from this region and, therefore, verification of the initial quantitative proteomics study in this second group of participants from a distant geographical region increases the strength of our findings and suggests a common host-acquired immune response to TB. Recently, mass spectrometry has been used to study the effect of phosphorylation in mycobacterial proteins known to be involved in MTB virulence 53 .…”

Section: Discussionsupporting

confidence: 56%

Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection

Mateos

Estévez

González-Fernández

et al. 2020

Sci Rep

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Tuberculosis (TB) is the most lethal infection among infectious diseases. The specific aim of this study was to establish panels of serum protein biomarkers representative of active tB patients and their household contacts who were either infected (LtBi) or uninfected (eMi-tB Discovery cohort, Pontevedra Region, Spain). A TMT (Tamdem mass tags) 10plex-based quantitative proteomics study was performed in quintuplicate containing a total of 15 individual serum samples per group. Peptides were analyzed in an Lc-orbitrap elite platform, and raw data were processed using proteome Discoverer 2.1. A total of 418 proteins were quantified. The specific protein signature of active tB patients was characterized by an accumulation of proteins related to complement activation, inflammation and modulation of immune response and also by a decrease of a small subset of proteins, including apolipoprotein A and serotransferrin, indicating the importance of lipid transport and iron assimilation in the progression of the disease. This signature was verified by the targeted measurement of selected candidates in a second cohort (EMI-TB Verification Cohort, Maputo Region, Mozambique) by ELISA and nephelometry techniques. These findings will aid our understanding of the complex metabolic processes associated with tB progression from LtBi to active disease. Tuberculosis (TB) is currently a global pandemic that kills approximately 1.3 million people worldwide each year 1. Although the incidence of TB is decreasing each year, the rate of that decline is not high enough to accomplish the TB Global Strategy for the 2015-2035 period 2,3. Mycobacterium tuberculosis (MTB) is the causal agent of the pulmonary TB 4 , which is air-transmitted by nasal/oral inhalation of aerosol droplets carrying the pathogen from an active TB patient to a healthy individual usually through coughing or sneezing 5. Small droplets that are able to reach the lower lung induce formation of a granuloma 6 , which is a host-defensive structure providing, a fibrotic physical barrier between the infected and the healthy neighboring tissues 7. Latently infected individuals (LTBI) represent roughly a quarter of the global population 8. Tuberculin Skin Test (TST) and Interferon-Gamma Release Assay (IGRA) are commonly used for diagnosis 9,10 of LTBI individuals. Although they cannot infect a healthy person, MTB activation occurs in roughly 10% of cases due to recurrent infections or a compromised immune system in the host 11 .

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Section: Discussionsupporting

confidence: 56%

Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection

Mateos

Estévez

González-Fernández

et al. 2020

Sci Rep

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“…Despite significant advances in the prevention and treatment of infectious diseases, expanding day-to-day, however, infections are still one of the leading causes of death, worldwide [1]. During the past few years, the number of just approved antibacterial drugs with a new mechanism of action becomes to decline [2, 3].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic applications of nucleic acid aptamers in microbial infections

et al. 2020

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Today, the treatment of bacterial infections is a major challenge, due to growing rate of multidrug-resistant bacteria, complication of treatment and increased healthcare costs. Moreover, new treatments for bacterial infections are limited. Oligonucleotide aptamers are single stranded DNAs or RNAs with target-selective high-affinity feature, which considered as nucleic acid-based affinity ligands, replacing monoclonal antibodies. The aptamer-based systems have been found to be talented tools in the treatment of microbial infections, regarding their promising anti-biofilm and antimicrobial activities; they can reduce or inhibit the effects of bacterial toxins, and inhibit pathogen invasion to immune cell, as well as they can be used in drug delivery systems. The focus of this review is on the therapeutic applications of aptamers in infections. In this regard, an introduction of infections and related challenges were presented, first. Then, aptamer definition and selection, with a brief history of aptamers development against various pathogens and toxins were reviewed. Diverse strategies of aptamer application in drug delivery, as well as, the effect of aptamers on the immune system, as the main natural agents of human defense against pathogens, were also discussed. Finally, the future trends in clinical applications of this technology were discussed.

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“…36 In the last two decades much attention has been paid to study the proteome of different human pathogenic bacteria. 37,38 S. marcescens is one of the pathogenic bacteria and many strains of this bacterium have been sequenced at genome and proteome levels. 10,18,20,22,[39][40][41] To the best of our knowledge, the genome of S. marcescens (ATCC 13880) sequence is available and the proteome of the same is not available till date.…”

Section: Discussionmentioning

confidence: 99%

Proteomic profiling of Serratia marcescens by high-resolution mass spectrometry

2020

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Introduction: Serratia marcescens, an opportunistic human pathogen, is reported as an important cause of nosocomial infection and outbreaks. Although the genome of S. marcescens (ATCC 13880) was completely sequenced by 2014, there are no studies on the proteomic profile of the organism. The objective of the present study is to analyze the protein profile of S. marcescens (ATCC 13880) using a high resolution mass spectrometry (MS). Methods: Serratia marcescens ATCC 13880 strain was grown in Luria-Bertani broth and the protein extracted was subjected to trypsin digestion, followed by basic reverse phase liquid chromatography fractionation. The peptide fractions were then analysed using Orbitrap Fusion Mass Spectrometry and the raw MS data were processed in Proteome Discoverer software. Results: The proteomic analysis identified 15 009 unique peptides mapping to 2541 unique protein groups, which corresponds to approximately 54% of the computationally predicted protein-coding genes. Bioinformatic analysis of these identified proteins showed their involvement in biological processes such as cell wall organization, chaperone-mediated protein folding and ATP binding. Pathway analysis revealed that some of these proteins are associated with bacterial chemotaxis and beta-lactam resistance pathway. Conclusion: To the best of our knowledge, this is the first high-throughput proteomics study of S. marcescens (ATCC 13880). These novel observations provide a crucial baseline molecular profile of the S. marcescens proteome which will prove to be helpful for the future research in understanding the host-pathogen interactions during infection, elucidating the mechanism of multidrug resistance, and developing novel diagnostic markers or vaccine for the disease.

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Editorial: Proteomics of Microbial Human Pathogens

Cited by 10 publications

References 4 publications

Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection

Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection

Therapeutic applications of nucleic acid aptamers in microbial infections

Proteomic profiling of Serratia marcescens by high-resolution mass spectrometry

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