Editorial: Golgi Dynamics in Physiological and Pathological Conditions

Prydz, Kristian; Lupashin, Vladimir; Wang, Yanzhuang; Saraste, Jaakko

doi:10.3389/fcell.2020.00007

Cited by 5 publications

(1 citation statement)

References 17 publications

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“…It receives newly synthesized proteins and lipids from the endoplasmic reticulum, sequentially modifies, and dispatches them to distinct destinations by protein sorting at the trans-Golgi network (TGN) (Klute et al, 2011;Brandizzi and Barlowe, 2013). Much of the recent efforts to define the mechanism of Golgi structure and function have been put on Golgi structural proteins including Golgins and GRASPs, actin and cytoskeletons, and other GTPases (Prydz et al, 2020). Interestingly, myosin 1C also regulated the Golgi structure and function.…”

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confidence: 99%

Tetramethylpyrazine Ameliorates Peritoneal Angiogenesis by Regulating VEGF/Hippo/YAP Signaling

Zhu

Shan

et al. 2021

Front. Pharmacol.

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Angiogenesis of human peritoneal vascular endothelial cells (HPVECs), linked to vascular endothelial growth factor (VEGF)/VEGF receptor 2 (VEGFR2) signaling, is a complication of peritoneal fibrosis (PF). Hippo/YAP signaling interacts with VEGF/VEGFR2 signaling, but the effect on peritoneal angiogenesis and PF has not been studied. We tested VEGF/Hippo/YAP inhibition by tetramethylpyrazine (TMP) in PF mice and HPVECs. This treatment ameliorated peritoneal dialysis (PD)–induced angiogenesis and PF. In mice, PF was associated with upregulation of VEGF, and TMP ameliorated submesothelial fibrosis, perivascular bleeding, and Collagen I abundance. In HPVECs, angiogenesis occurred due to human peritoneal mesothelial cells (HPMCs)–conditioned medium, and TMP alleviated HPVECs migration, tube formation, and YAP nuclear translocation. YAP knockdown PF mouse and HPVEC models were established to further confirm our finding. YAP deletion attenuated the PD-induced or VEGF-induced increase in angiogenesis and PF. The amount of CYR61 and CTGF was significantly less in the YAP knockdown group. To study the possibility that TMP could benefit angiogenesis, we measured the HPVECs migration and tube formation and found that both were sharply increased in YAP overexpression; TMP treatment partly abolished these increases. As well, the amount of VEGFR localized in the trans-Golgi network was lower by double immunofluorescence; VEGFR and its downstream signaling pathways including p-ERK, p-P38, and p-Akt were more in HPVECs with YAP overexpression. Overall, TMP treatment ameliorated angiogenesis, PF, and peritoneum injury. These changes were accompanied by inhibition of VEGF/Hippo/YAP.

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confidence: 99%