Editorial

Doerr, Allison; Finkelstein, Joshua M.; Jarchum, Irene; Goodman, Catherine; Dekker, Bronwen A

doi:10.1038/nmeth.3558

Cited by 5 publications

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“…Mass spectrometry (MS) with electrospray ionization (ESI) source is a key technique in various research fields, ranging from food and environmental analysis to metabolomics and proteomics . However, despite its widespread application, current understanding of the ESI process is still limited. , …”

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“…M ass spectrometry (MS) with electrospray ionization (ESI 1 ) source is a key technique in various research fields, ranging from food and environmental analysis to metabolomics and proteomics. 2 However, despite its widespread application, current understanding of the ESI process is still limited. 3,4 Ionization efficiency (IE), the amount of ions generated from a specific compound in the ionization source, may vary from compound to compound by more than 6 orders of magnitude.…”

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Think Negative: Finding the Best Electrospray Ionization/MS Mode for Your Analyte

et al. 2017

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For the first time, the electrospray ionization efficiency (IE) scales in positive and negative mode are united into a single system enabling direct comparison of IE values across ionization modes. This is made possible by the use of a reference compound that ionizes to a similar extent in both positive and negative modes. Thus, choosing the optimal (i.e., most sensitive) ionization conditions for a given set of analytes is enabled. Ionization efficiencies of 33 compounds ionizing in both modes demonstrate that, contrary to general practice, negative mode allows better sensitivity for 46% of such compounds whereas the positive mode is preferred for only 18%, and for 36%, the results for both modes are comparable.

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Think Negative: Finding the Best Electrospray Ionization/MS Mode for Your Analyte

et al. 2017

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Introduction

Gross¹

2017

Mass Spectrometry

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Assessing the Structural Heterogeneity of Isomeric Homo and Copolymers: an Approach Combining Ion Mobility Mass Spectrometry and Molecular Dynamics Simulations

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Moins

Coulembier

et al. 2020

J. Am. Soc. Mass Spectrom.

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Synthetic polymers occupy a unique place in the field of ion mobility mass spectrometry (IMS−MS). Indeed, due to their intrinsic dispersity, they have the asset to offer a broad range of homologous ions with different lengths that can be detected in several charge states. In addition, the gas-phase structure of polymer ions mostly depends on their ability to screen the adducted charges. Several works dealing with linear, cyclic, and star-shaped polymers have already shown that the gas-phase structure of polymer ions heavily relies on the polymer architecture, i.e., the primary structure. In the present work, we move a step further by evaluating whether a relationship exists between the primary and secondary structures of synthetic homo and copolymers. The IMS−MS experiments will be further complemented by MD simulations. To highlight the effectiveness of IMS separation, we selected isomeric homo and copolymers made of lactide (LA) and propiolactone (PL) units. In this way, the mass analysis becomes useless since isomeric comonomer sequences can coexist for any given chain length. An UPLC method was implemented in the workflow to successfully separate all PL−LA comonomer sequences before infusion in the IMS−MS instrument. The analysis of doubly charged copolymers showed that the comonomer sequence has an impact on the IMS response. However, this only holds for copolymer ions with precise sizes and charge states, and this is therefore not a rule of thumb.

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Editorial

Cited by 5 publications

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Think Negative: Finding the Best Electrospray Ionization/MS Mode for Your Analyte

Think Negative: Finding the Best Electrospray Ionization/MS Mode for Your Analyte

Introduction

Assessing the Structural Heterogeneity of Isomeric Homo and Copolymers: an Approach Combining Ion Mobility Mass Spectrometry and Molecular Dynamics Simulations

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