Editing plant genomes with CRISPR/Cas9

Generation of targeted mutant rice using a CRISPR‐Cpf1 system

“…The CRISPR‐Cas9 system has emerged as a precise and versatile technique for genome editing, and mounting research demonstrates that efficient editing could be induced by this system in various plant organisms, such as Arabidopsis, rice, wheat, soya bean, maize, tobacco and tomato (Belhaj et al ., 2015; Bortesi and Fischer, 2015; Liu et al ., 2016). Furthermore, the development and customization of plant CRISPR/Cas9 toolboxes or platforms result in more powerful molecular tools for fundamental research and crop breeding (Lowder et al ., 2015; Ma et al ., 2015; Mao et al ., 2016; Tang et al ., 2016; Wang et al ., 2015; Xie et al ., 2015; Xing et al ., 2014).…”

Advancing biotechnology with CRISPR/Cas9: recent applications and patent landscape

“…In the field of crop engineering, CRISPR techniques are currently having a major impact, facilitating cheaper, faster, and more precise engineering in comparison to laborious and time-consuming traditional methods [3,82]. However, it has yet to be determined whether CRISPR based gene editing of crops will be regulated the same way traditional genetically engineered crops are, which ultimately will settle its commercial value within this sector.…”

Genome Editing with Engineered Nucleases in Economically Important Animals and Plants: State of the Art in the Research Pipeline

“…The second mechanism, homologous recombination (HR), is based on a template homologous to the sequence surrounding the DSB. The template is present in the chromosome in case of a naturally occurring HR; however, if an external template is delivered, the HR can be used to make custom changes to the genome including insertions of an exogenous DNA sequence (Harrison et al, 2014;Belhaj et al, 2015;Bortesi and Fischer, 2015). Due to the provided template, the change made by HR is usually exact (Belhaj et al, 2015); however, compared to NHEJ, it occurs…”

“…The CRISPR/Cas9 system is the most recent of the SDNs (Boettcher and McManus, 2015); the d e s i g n i s s t r a i g h t f o r w a r d a n d c a n b e accomplished quickly as the only part that needs redesign is the guide RNA (Puchta and Fauser, 2014). The CRISPR/Cas9 system also allows multiplexing to generate organisms with multiple mutations or large chromosomal deletions (Belhaj et al, 2015). The CRISPR/Cas9 system and TALENs are the most promising SDNs.…”