Editing efficiencies with Cas9 orthologs, Cas12a endonucleases, and temperature in rice

Illa-Berenguer, Eudald; LaFayette, Peter R.; Parrott, W. A.

doi:10.3389/fgeed.2023.1074641

Cited by 3 publications

(2 citation statements)

References 91 publications

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“…The next level is epigenetic editors that do not change the genome sequence and affect the activity of genes at the levels of their transcription, splicing and translation [318]. SpCas9 orthologues from other bacteria are also used, as well as CRISPR/Cas systems of other types [319]. A second frequently used edit is Cas12a [320], a smaller protein that has only one nuclease domain and, therefore, leaves sticky rather than blunt ends.…”

Section: Development Of Sustainable Geneticallymentioning

confidence: 99%

Editing Metabolism, Sex, and Microbiome: How Can We Help Poplar Resist Pathogens?

Kovalev,

Gladysh,

Bogdanova

et al. 2024

IJMS

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Poplar (Populus) is a genus of woody plants of great economic value. Due to the growing economic importance of poplar, there is a need to ensure its stable growth by increasing its resistance to pathogens. Genetic engineering can create organisms with improved traits faster than traditional methods, and with the development of CRISPR/Cas-based genome editing systems, scientists have a new highly effective tool for creating valuable genotypes. In this review, we summarize the latest research data on poplar diseases, the biology of their pathogens and how these plants resist pathogens. In the final section, we propose to plant male or mixed poplar populations; consider the genes of the MLO group, transcription factors of the WRKY and MYB families and defensive proteins BbChit1, LJAMP2, MsrA2 and PtDef as the most promising targets for genetic engineering; and also pay attention to the possibility of microbiome engineering.

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Section: Development Of Sustainable Geneticallymentioning

confidence: 99%

Editing Metabolism, Sex, and Microbiome: How Can We Help Poplar Resist Pathogens?

Kovalev,

Gladysh,

Bogdanova

et al. 2024

IJMS

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“…As gene knockout technology matures, CRISPR-Cas9 genome editing has rapidly become an important tool in genomic research [19,20]. During the repair process, mismatching via base insertion or deletion usually occurs, resulting in code-shifting mutations.…”

Section: Of 13mentioning

confidence: 99%

Knocking out OsNAC050 Expression Causes Low-Temperature Tolerance in Rice by Regulating Photosynthesis and the Sucrose Metabolic Pathway

Wang

Xie

et al. 2023

Agriculture

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Because rice is native to tropical and subtropical regions, it is generally more sensitive to cold stress compared to other cereals. Low-temperature stress has a strong influence on the growth, development, and yield of rice. Plant NAM, ATAF1/2 and CUC(NAC) transcription factors (TFs) play significant regulatory roles in stress response. In our research, we found that OsNAC050 (LOC_Os03g60080) is mainly expressed in leaves. Cold (4 °C), heat (42 °C), PEG 6000 (20%, w/v), NaCl (200 mM), H2O2 (1%), IAA (100 μM), ABA (100 μM) and GA3 (100 μM) were used to treat wild type (WT) plants. A low temperature further up-regulated OsNAC050 expression. OsNAC050 mutants created using CRISPR-Cas9 gene editing technology showed significantly enhanced tolerance to the low-temperature treatment. The measurement of enzyme activities related to the redox pathway also showed that mutants have stronger viability under low-temperature stress. Comparative transcriptome analysis showed that photosynthesis and soluble sugar metabolism were significantly affected in OsNAC050 mutant lines, suggesting that OsNAC050 may participate in the above molecular pathways in response to low-temperature stress. The results expand our understanding of the molecular mechanisms underlying the responses to cold stress in rice and can provide new strategies for engineering cold tolerance in high-yielding rice varieties.

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Knocking Out <em>OsNAC050</em> Expression Causes Low Temperature Tolerance in Rice by Regulating Photosynthesis and the Sucrose Metabolic Pathway

Wang¹,

Wang²,

Xie³

et al. 2023

Preprint

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Because rice is native to tropical and subtropical regions, it is generally more sensitive to cold stress compared to other cereals. Low temperature stress has become one of the most important conditions that affects the growth, development, and yield of rice. Plant NAC (NAM, ATAF1/2, and CUC) transcription factors are known to play significant regulatory roles in the stress response. In our research, we found that OsNAC050 (LOC_Os03g60080） is mainly expressed in leaves, and low temperature can further up-regulate its expression. OsNAC050 mutants created using CRISPR-Cas9 gene editing technology showed significantly enhanced tolerance to low temperature treatment. Detection of enzyme activities related to the redox pathway also showed that the mutants had stronger viability under low temperature stress. Comparative transcriptome analysis showed that photosynthesis and soluble sugar metabolism were significantly affected in the osnac050 mutant lines, suggesting that OsNAC050 may participate in the above molecular pathways in the response to low temperature stress. The results will enhance our understanding of molecular mechanisms underlying the responses to cold stress in rice and can provide new strategies for engineering cold-tolerance in high-yielding rice varieties.

show abstract

Editing efficiencies with Cas9 orthologs, Cas12a endonucleases, and temperature in rice

Cited by 3 publications

References 91 publications

Editing Metabolism, Sex, and Microbiome: How Can We Help Poplar Resist Pathogens?

Editing Metabolism, Sex, and Microbiome: How Can We Help Poplar Resist Pathogens?

Knocking out OsNAC050 Expression Causes Low-Temperature Tolerance in Rice by Regulating Photosynthesis and the Sucrose Metabolic Pathway

Knocking Out <em>OsNAC050</em> Expression Causes Low Temperature Tolerance in Rice by Regulating Photosynthesis and the Sucrose Metabolic Pathway

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