Editing a Stomatal Developmental Gene in Rice with CRISPR/Cpf1

Yin, Xiaojia; Anand, Abhishek; Quick, Paul; Bandyopadhyay, Anindya

doi:10.1007/978-1-4939-8991-1_19

Cited by 23 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both antisense OsRBCS2 and OsRBCS4 vectors were verified by sequencing and transformed into rice ( Oryza sativa indica cv . IR64) using Agrobacterium -mediated transformation following the method described by Yin et al (2019) .…”

Section: Methodsmentioning

confidence: 99%

Targeted knockdown of ribulose-1, 5-bisphosphate carboxylase-oxygenase in rice mesophyll cells

Maheshwari

Coe

Karki

et al. 2021

Journal of Plant Physiology

View full text Add to dashboard Cite

We generated antisense constructs targeting two of the five Rubisco small subunit genes ( OsRBCS2 and 4 ) which account for between 30–40 % of the RBCS transcript abundance in leaf blades. The constructs were driven by a maize phosphoenolpyruvate carboxylase (PEPC) promoter known to have enriched expression in mesophyll cells (MCs). In the resulting lines leaf, Rubisco protein content was reduced by between 30–50 % and CO 2 assimilation rate was limited under photorespiratory and non-photorespiratory conditions. A relationship between Rubisco protein content and CO 2 assimilation rate was found. This was associated with a significant reduction in dry biomass accumulation and grain yield of between 37–70%. In addition to serving as a resource for reducing Rubisco accumulation in a cell-preferential manner, these lines allow us to characterize gene function and isoform specific suppression on photosynthesis and growth. Our results suggest that the knockdown of multiple genes is required to completely reduce Rubisco accumulation in MCs.

show abstract

Section: Methodsmentioning

confidence: 99%

Targeted knockdown of ribulose-1, 5-bisphosphate carboxylase-oxygenase in rice mesophyll cells

Maheshwari

Coe

Karki

et al. 2021

Journal of Plant Physiology

View full text Add to dashboard Cite

show abstract

“…Knocking out an independent gene, OsEPFL9 (Os01g0824500), results in increased water use efficiency under stress because of the reduced stomatal count (Yin et al, 2017(Yin et al, , 2019.…”

Section: Stress Tolerancementioning

confidence: 99%

Rice breeding in the new era: comparison of useful agronomic traits

Hernández-Soto

Echeverría-Beirute

Abdelnour-Esquivel

et al. 2021

Preprint

View full text Add to dashboard Cite

Understanding agronomic traits at a genetic level enables the leveraging of this knowledge to produce crops that are more productive and resilient, have better quality and are adjusted for consumer preferences. In the last decade, rice has become a model to validate the function of specific genes, resulting in valuable but scattered information. Here, we aimed to identify particular genes in rice related to traits that can be targeted by different mutation techniques in the breeding of crops. We selected gain of function, misfunction, and specific mutations associated with phenotypes of agronomic interest. The review includes specific trait-related genes involved in domestication, stress, herbicide tolerance, pathogen resistance, grain number/quality/weight, plant structure, nitrogen use, and others. The information presented can be used for rice, other cereals, and orphan crops to achieve a superior and sustainable production in challenging farming conditions.

show abstract

“…Other individual genes could be of interest, such as the Na+ transporter SKC1 (Os01g0307500) with a V395 that provides salt tolerance (Jayabalan et al, 2019). Knocking out an independent but closely related gene, OsEPFL9 (Os01g0824500), results in increased water use e ciency under stress because of the reduced stomatal count (Yin et al, 2017(Yin et al, , 2019.…”

Section: Stress Tolerancementioning

confidence: 99%

Rice agronomic traits and variability induced by mutagenesis

Hernández-Soto¹,

Echeverría-Beirute²,

Abdelnour-Esquivel³

et al. 2021

Preprint

View full text Add to dashboard Cite

Understanding agronomic traits at a genetic level enables the leveraging of this knowledge to produce crops that are more productive and resilient, have better quality and are adjusted for consumer preferences. In the last decade, rice has become a model to validate the function of specific genes, resulting in valuable but scattered information. Here, we aimed to identify particular genes in rice related to traits that can be targeted by different mutation techniques in the breeding of crops. We selected gain of function, misfunction, and specific mutations associated with phenotypes of agronomic interest. The review includes specific trait-related genes involved in domestication, stress, herbicide tolerance, pathogen resistance, grain number, quality, weight, plant structure, nitrogen use, and others. The information presented can be used for rice and crops with similar or homologous genes to breed crops that require improvement to achieve more sustainable production in challenging farming conditions.

show abstract

Editing a Stomatal Developmental Gene in Rice with CRISPR/Cpf1

Cited by 23 publications

References 31 publications

Targeted knockdown of ribulose-1, 5-bisphosphate carboxylase-oxygenase in rice mesophyll cells

Targeted knockdown of ribulose-1, 5-bisphosphate carboxylase-oxygenase in rice mesophyll cells

Rice breeding in the new era: comparison of useful agronomic traits

Rice agronomic traits and variability induced by mutagenesis

Contact Info

Product

Resources

About