Edinger-Westphal peptidergic neurons enable maternal preparatory nesting

Topilko, Thomas; Diaz, Silvina Laura; Pacheco, Catarina M.; Verny, Florine; Rousseau, Charly V.; Kirst, Christoph; Deleuze, Charlotte; Gaspar, Patricia; Renier, Nicolas

doi:10.1016/j.neuron.2022.01.012

Cited by 40 publications

(32 citation statements)

References 57 publications

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“…It is very striking that X*Y females display quite consistently divergent maternal behaviours: they show a greater retrieval efficiency, high level of maternal aggression and poor nesting skills, all of which differ from XX and XX* females. While the two formers correlate with a feminizing X* chromosome, the latter suggests masculinization with impaired nesting behaviour as found in males (data not shown; Topilko et al, 2022) due to the Y chromosome and Sry expression in X*Y females (Gatewood et al, 2006. Interestingly, this pattern of both masculinized and hyper-feminized traits was already present in previous studies on the consequences of the sex reversal in M. minutoides.…”

Section: Discussionsupporting

confidence: 74%

“…It is very striking that X*Y females displayed -quite consistently-, divergent maternal behaviours from XX and XX* females: they showed a greater retrieval efficiency, high level of maternal aggression but also poor nesting skills as found in males suggesting masculinization (data not shown; Topilko et al, 2022). Interestingly, this pattern of co- occurrence of both feminized and masculinized traits in M. minutoides X*Y females was already found in previous studies.…”

Section: Discussionmentioning

confidence: 89%

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Genotypic sex shapes maternal care in the African Pygmy mouse,Mus minutoides

Heitzmann

Challe

Pérez

et al. 2022

Preprint

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Parental care, one of the most sexually dimorphic behaviour in mammals, was long thought to be driven mostly, if not exclusively, by gonadal hormones. Over the past two decades, very few studies have challenged this view, highlighting the direct influence of the sex chromosome complement (XX vs XY). The African pygmy mouse, Mus minutoides , is a wild mouse species with a naturally occurring sex reversal due to a third, feminizing X* chromosome, leading to three female genotypes: XX, XX* and X*Y. Here, we show that the sex reversal in X*Y females shapes a divergent maternal care strategy from both XX and XX* females, rather than altering care quality. In addition, the sex reversal is likely to impact the dopaminergic system in the anteroventral periventricular nucleus of the hypothalamus, consistent with one component of maternal care: pup retrieval. Combining neurobiology and behavioural ecology in a wild mouse species subject to natural selection, we evaluate here the neural basis of maternal behaviours and strengthen the underestimated role of the sex chromosome in shaping sex differences in brain and behaviours. Furthermore, we highlight the emergence of a third sexual phenotype, challenging the binary view of sexes.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 89%

Genotypic sex shapes maternal care in the African Pygmy mouse,Mus minutoides

Heitzmann

Challe

Pérez

et al. 2022

Preprint

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“…Cux2 and Satb2 in L2/3_A, Rorb and Rspo1 in L4, Fezf2 and Scube1 in L5, and Col5a1 and Rprm in L6) and hippocampal pyramidal layer markers (e.g., Fibcd1 in CA1, Rgs14 in CA2, Npy2r in CA3, and Prox1 in DG). Importantly, we were able to capture gene signatures in very fine structures of brain nuclei, such as Cartpt in the Edinger-Westphal nucleus (EW), Chrna2 in the interpeduncular nucleus (IPN), and Rgs16 in the hypothalamic suprachiasmatic nucleus (SCH), in line with the previous literature 38–40 (Extended Data Fig. 6b-d).…”

Section: Introductionsupporting

confidence: 78%

Spatial Atlas of the Mouse Central Nervous System at Molecular Resolution

Shi

Zhou

et al. 2022

Preprint

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Spatially charting molecular cell types at single-cell resolution across the three-dimensional (3D) volume of the brain is critical for illustrating the molecular basis of the brain anatomy and functions. Single-cell RNA sequencing (scRNA-seq) has profiled molecular cell types in the mouse brain, but cannot capture their spatial organization. Here, we employed an in situ sequencing technique, STARmap PLUS, to map more than one million high-quality cells across the whole adult mouse brain and the spinal cord, profiling 1,022 genes at subcellular resolution with a voxel size of 194 X 194 X 345 nm in 3D. We developed computational pipelines to segment, cluster, and annotate molecularly defined cell types and tissue regions with single-cell resolution. To create a transcriptome-wide spatial atlas, we further integrated the STARmap PLUS measurements with a published scRNA-seq atlas, imputing 11,844 genes at the single-cell level. Finally, we engineered a highly expressed RNA barcoding system to delineate the tropism of a brain-wide transgene delivery tool, AAV-PHP.eB, revealing its single-cell resolved transduction efficiency across the molecular cell types and tissue regions of the whole mouse brain. Together, our datasets and annotations provide a comprehensive, high-resolution single-cell resource that integrates spatial molecular atlas, cell taxonomy, brain anatomy, and genetic manipulation accessibility of the mammalian central nervous system (CNS).

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“…Next, a prominent cluster selectively expressed Ttc6, which we found to be highly expressed in the red nucleus in Atlas images ( Figure 4I-L ). Cartpt is a known marker for the Edinger Westphal nucleus (EW) (Topilko et al, 2022; Xu et al, 2014), and was selectively expressed in a small cluster ( Figure 4M-P ). Another small cluster expressed Slc6a2, a well-established marker for noradrenergic neurons, and thus likely corresponds to the locus coeruleus (Mulvey et al, 2018) ( Figure 4Q-T ).…”

Section: Resultsmentioning

confidence: 99%

Single nuclei analyses reveal transcriptional profiles and marker genes for diverse supraspinal populations

Beine

Wang

Tsoulfas

et al. 2022

Preprint

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The mammalian brain contains numerous neurons distributed across forebrain, midbrain, and hindbrain that project axons to the lower spinal cord and work in concert to control movement and achieve homeostasis. Extensive work has mapped the anatomical location of supraspinal cell types and continues to establish specific physiological functions. The patterns of gene expression that typify and distinguish these disparate populations, however, are mostly unknown. Here we combined retrograde labeling of supraspinal cell nuclei with fluorescence activated nuclei sorting and single nuclei RNA sequencing analyses to transcriptionally profile neurons that project axons from the mouse brain to lumbar spinal cord. We identified fourteen transcriptionally distinct cell types and used a combination of established and newly identified marker genes to assign an anatomical location to each. To validate the putative marker genes, we visualized selected transcripts and confirmed selective expression within lumbar-projecting neurons in discrete supraspinal regions. Finally, we illustrate the potential utility of these data by examining the expression of transcription factors that distinguish different supraspinal cell types and by surveying the expression of receptors for growth and guidance cues that may be present in the spinal cord. Collectively these data establish transcriptional differences between anatomically defined supraspinal populations, identify a new set of marker genes of use in future experiments, and provide insight into potential differences in cellular and physiological activity across the supraspinal connectome.

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Edinger-Westphal peptidergic neurons enable maternal preparatory nesting

Cited by 40 publications

References 57 publications

Genotypic sex shapes maternal care in the African Pygmy mouse,Mus minutoides

Genotypic sex shapes maternal care in the African Pygmy mouse,Mus minutoides

Spatial Atlas of the Mouse Central Nervous System at Molecular Resolution

Single nuclei analyses reveal transcriptional profiles and marker genes for diverse supraspinal populations

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