EDIII‐DENV3 nanospheres drive immature dendritic cells into a mature phenotype in an <i>in vitro</i> model

Nantachit, Nattika; Sunintaboon, Panya; Ubol, Sukathida

doi:10.1111/1348-0421.12497

Cited by 14 publications

(13 citation statements)

References 52 publications

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“…Treatment with empty TMC nPs also up-regulated expression of maturation markers at significantly higher levels than medium-treated cells. Previous studies showed that TMC nPs were able to stimulate DC maturation [ 24 , 71 ]. Accordingly, it might be possible that these act synergistically to induce MoDCs maturation.…”

Section: Discussionmentioning

confidence: 99%

Development of an adjuvanted nanoparticle vaccine against influenza virus, an in vitro study

et al. 2020

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Influenza is an infectious respiratory illness caused by influenza viruses. Despite yearly updates, the efficacy of influenza vaccines is significantly curtailed by the virus antigenic drift and antigenic shift. These constant changes to the influenza virus make-up also challenge the development of a universal flu vaccine, which requires conserved antigenic regions shared by influenza viruses of different subtypes. We propose that it is possible to bypass these challenges by the development of an influenza vaccine based on conserved proteins delivered in an adjuvanted nanoparticle system. In this study, we generated influenza nanoparticle constructs using trimethyl chitosan nanoparticles (TMC nPs) as the carrier of recombinant influenza hemagglutinin subunit 2 (HA2) and nucleoprotein (NP). The purified HA2 and NP recombinant proteins were encapsulated into TMC nPs to form HA2-TMC nPs and NP-TMC nPs, respectively. Primary human intranasal epithelium cells (HNEpCs) were used as an in vitro model to measure immunity responses. HA2-TMC nPs, NP-TMC nPs, and HA2-NP-TMC nPs (influenza nanoparticle constructs) showed no toxicity in HNEpCs. The loading efficiency of HA2 and NP into the TMC nPs was 97.9% and 98.5%, respectively. HA2-TMC nPs and NP-TMC nPs more efficiently delivered HA2 and NP proteins to HNEpCs than soluble HA2 and NP proteins alone. The induction of various cytokines and chemokines was more evident in influenza nanoparticle construct-treated HNEpCs than in soluble protein-treated HNEpCs. In addition, soluble factors secreted by influenza nanoparticle construct-treated HNEpCs significantly induced MoDCs maturation markers (CD80, CD83, CD86 and HLA-DR), as compared to soluble factors secreted by protein-treated HNEpCs. HNEpCs treated with the influenza nanoparticle constructs significantly reduced influenza virus replication in an in vitro challenge assay. The results indicate that TMC nPs can be used as influenza vaccine adjuvants and carriers capable of delivering HA2 and NP proteins to HNEpCs.

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Section: Discussionmentioning

confidence: 99%

Development of an adjuvanted nanoparticle vaccine against influenza virus, an in vitro study

et al. 2020

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“…The EE and LE of the nanospheres were determined (Yang et al, 2017). The nanospheres were characterized using FTIR (Abbas et al, 2018;Dong et al, 2018), electron microscopy (Shi et al, 2016;Nantachit et al, 2017;Tao et al, 2018), and laser particle size analysis (Couvreur et al, 2002;Zhang et al, 2015;Shi et al, 2016;Ahmad et al, 2018;Panchu et al, 2018;von Halling Laier et al, 2018). The results showed that C-PC/CMC-CD55sp nanospheres were successfully constructed.…”

Section: Discussionmentioning

confidence: 99%

“…Encapsulation Efficiency (EE) and Loading Efficiency (LE) of C-PC in nanospheres were calculated as follows (Yang et al, 2017): EE = weight of C − PC in the nanospheres initial weight of C − PC Â 100 % LE = weight of C − PC in the nanospheres weight of the nanospheres Â 100 % Nanospheres were evaluated using FTIR spectra across the range of 500-4,000 cm -1 (Abbas et al, 2018;Dong et al, 2018). Nanospheres morphology was observed using a transmission electron microscope (Shi et al, 2016;Nantachit et al, 2017;Tao et al, 2018). Zeta potential (surface charge) and particle size (nanospheres size) were measured using a laser particle size analyzer at 25°C (Zhang et al, 2015;Ahmad et al, 2018;Panchu et al, 2018;von Halling Laier et al, 2018).…”

Section: Synthesis Of C-pc/cmc-cd55sp Nanospheresmentioning

confidence: 99%

Targeted Antitumor Mechanism of C-PC/CMC-CD55sp Nanospheres in HeLa Cervical Cancer Cells

Liu

Jiang

et al. 2020

Front. Pharmacol.

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In vitro studies had shown that C-Phycocyanin (C-PC) inhibited cervical cancer HeLa cells growth. We constructed C-PC/CMC-CD55sp nanospheres using C-PC, Carboxymethyl Chitosan (CMC), and CD55 ligand peptide (CD55sp) to allow for targeted antitumor effects against HeLa cells in vitro and in vivo. The characteristics of the nanospheres were determined using FTIR, electron microscopy, and laser particle size analysis. Flow cytometry, laser confocal microscopy and small animal imaging system showed the targeting of C-PC/CMC-CD55sp nanospheres on HeLa cells. Subsequently, the proliferation and apoptosis were analyzed by Cell Counting Kit-8 (CCK-8), flow cytometry, TUNEL assay and electron microscopy. The expression of the apoptosisrelated protein was determined using western blot. The stainings of Hematoxylin and Eosin (HE) were employed to evaluate the cell condition of tumor tissue sections. The cytokines in the blood in tumor-bearing nude mice was determined using ELISA. These results showed that C-PC/CMC-CD55sp nanospheres were successfully constructed and targeted HeLa cells. The constructed nanospheres were more effective than C-PC alone in inhibiting the proliferation and inducing apoptosis in HeLa cells. We also found that C-PC/CMC-CD55sp nanospheres had a significant inhibitory effect on the expression of antiapoptotic protein Bcl-2 and a promotion on the transformation of caspase 3 to cleaved caspase 3. C-PC/CMC-CD55sp nanospheres played an important role in tumor suppression, reduced the expression TGF-b, and increased IL-6 and TNF-a. This study demonstrates that the constructed new C-PC/CMC-CD55sp nanospheres exerted targeted antitumor effects in vivo and in vitro which provided a novel idea for application of C-PC, and provided experimental basis for comprehensive targeted treatment of tumors.

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“…Overall, in vitro models have proved to be a useful tool to study MBF, with examples that study virus molecular mechanisms (Correa et al, 2015; Londono-Renteria et al, 2017; Rattanamahaphoom et al, 2017), their implication in the inflammatory response (Lim et al, 2014; Duran et al, 2016), drug screening (Stolp et al, 2015; Wang L. F. et al, 2015; Sayce et al, 2016), or vaccine development (Nantachit et al, 2016, 2017; Kam et al, 2017). Thus, human cell-derived in vitro models could present a solution to modeling MBF infection and neuropathogenesis.…”

Section: In Vitro Studiesmentioning

confidence: 99%

In vitro and in silico Models to Study Mosquito-Borne Flavivirus Neuropathogenesis, Prevention, and Treatment

Chesnut

Muñoz

Harris

et al. 2019

Front. Cell. Infect. Microbiol.

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Mosquito-borne flaviviruses can cause disease in the nervous system, resulting in a significant burden of morbidity and mortality. Disease models are necessary to understand neuropathogenesis and identify potential therapeutics and vaccines. Non-human primates have been used extensively but present major challenges. Advances have also been made toward the development of humanized mouse models, but these models still do not fully represent human pathophysiology. Recent developments in stem cell technology and cell culture techniques have allowed the development of more physiologically relevant human cell-based models. In silico modeling has also allowed researchers to identify and predict transmission patterns and discover potential vaccine and therapeutic candidates. This review summarizes the research on in vitro and in silico models used to study three mosquito-borne flaviviruses that cause neurological disease in humans: West Nile, Dengue, and Zika. We also propose a roadmap for 21st century research on mosquito-borne flavivirus neuropathogenesis, prevention, and treatment.

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EDIII‐DENV3 nanospheres drive immature dendritic cells into a mature phenotype in an in vitro model

Cited by 14 publications

References 52 publications

Development of an adjuvanted nanoparticle vaccine against influenza virus, an in vitro study

Development of an adjuvanted nanoparticle vaccine against influenza virus, an in vitro study

Targeted Antitumor Mechanism of C-PC/CMC-CD55sp Nanospheres in HeLa Cervical Cancer Cells

In vitro and in silico Models to Study Mosquito-Borne Flavivirus Neuropathogenesis, Prevention, and Treatment

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