Edible Growth Medium: A New Window for Probiotic Research

Hossain, Md. Nur; Akter, Annana; Humayan, Sanjida; Mohanto, Liton Chandra; Begum, Shamima; Ahmed, Monzur Morshed

doi:10.4236/aim.2020.102004

Cited by 6 publications

(4 citation statements)

References 20 publications

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“…and Bifidobacteria sp. [27]. In agreement with these results was the cultivable community structure of bacteria identified from both root and inner tissues of maize as well as rice seedlings [59,60].…”

Section: Discussionsupporting

confidence: 77%

Plant Broth- (Not Bovine-) Based Culture Media Provide the Most Compatible Vegan Nutrition for In Vitro Culturing and In Situ Probing of Plant Microbiota

et al. 2020

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Plant microbiota support the diversity and productivity of plants. Thus, cultivation-dependent approaches are indispensable for in vitro manipulation of hub taxa. Despite recent advances in high-throughput methods, cultivability is lagging behind other environmental microbiomes, notably the human microbiome. As a plant-based culturing strategy, we developed culture media based on a broth of cooked aqueous mixtures of host plants. This improved the in vitro growth of representative isolates of plant microbiota and extended the in situ recovery of plant microbiota. With clover, 16S rRNA gene sequencing of representative isolates confirmed the predominance of Firmicutes, Alphaproteobacteria and Gammaproteobacteria, and less frequently Bacteroidetes and Actinobacteria. Whereas bovine-based culture media (modified R2A) confined the diversity to Firmicutes, the plant broth-based culture media revealed a wider scope of endophytes beyond rhizobia, i.e., multiple genera such as Chryseobacterium, Cronobacter, Kosakonia, Tsukamurella, and a potentially/presumptive novel species. Matrix-assisted laser desorption/ionization time-of-flight (MADI-TOF) analysis clustered isolates according to their plant niches, the endo-phyllosphere/endo-rhizosphere. We recommend the plant broth for simplicity, reproducibility and perdurable storage, supporting future culturomics applications, good laboratory practice (GLP) and good manufacturing practice (GMP). The strategy creates an “in-situ-similis” vegan nutritional matrix to analyze microbial diversity and reveal novel microbial resources pertinent to biotechnological and environmental applications.

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Section: Discussionsupporting

confidence: 77%

Plant Broth- (Not Bovine-) Based Culture Media Provide the Most Compatible Vegan Nutrition for In Vitro Culturing and In Situ Probing of Plant Microbiota

et al. 2020

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“…Conversely, LAB cultured in almond broth exhibited the lowest cell viability (0.90 ± 0.02 × 10 8 CFU/mL). The superior performance of mung bean and coconut media can be attributed to their higher carbohydrate content than other plant-based media (Hossain et al 2020). Additionally, our results showed that GABA produced by LAB cultured in these media was lower than in MRS broth.…”

Section: Effect Of Fermentation Of Co-cultured Lab In Different Types...mentioning

confidence: 64%

Production of Postbiotic Metabolites γ-Aminobutyric Acid (GABA) and Bacteriocin-Like Inhibitory Substances (BLIS) by Lactobacillus brevis C23 Co-culture in Coconut-Based Medium for Potential Psychobiotics and Food Preservatives

Chuah

Tan

Oslan

et al. 2023

Preprint

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Lactic acid bacteria (LAB) can produce γ-aminobutyric acid (GABA) with antioxidant properties and sedative effects when it binds to GABA receptor in human’s brain. LAB can also produce bacteriocin-like inhibitory substances (BLIS) with antimicrobial capabilities during carbohydrate fermentation. GABA and BLIS are natural compounds with potential health benefits and food preservation properties. Lactobacillus brevis C23 was co-cultured with three different LAB as inducers, which produced the highest GABA content and BLIS activity. They were cultured in various plant-based media to obtain an edible and better-tasting final product over commercially available media like MRS broth. A coconut-based medium with additives was optimized using Response Surface Methodology (RSM) to increase GABA and BLIS production. The optimized medium for maximum GABA production (3.22 ± 0.01mg/mL) and BLIS activity (84.40 ± 0.44%) was a 5.5% coconut medium containing 0.23% glucose, 1.44% Tween 20, 0.48% L-glutamic acid and 0.02% pyridoxine. Due to the presence of GABA, the cell-free supernatant (CFS) as a postbiotic showed a higher antioxidant activity than other food preservatives like nisin and potassium sorbate. Finally, a microbiological test on food samples showed that the postbiotic was more effective than other preservatives at combating the growth of LAB, molds, and coliform bacteria, making it a possible food preservative.

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“…bulgaricus warrants supplementing the growth media with amino acids, peptides and vitamins, hence, yeast extracts are recommended as complex nitrogen sources for growth promotion (Atilola et al , 2015; Curk et al ., 1993; Klotz et al ., 2017). Bacterial growth in the MRS and KCMax media blends suggested their nitrogen content impacted bacterial growth (Hossain et al ., 2020; Papizadeh et al , 2020). Generally, nutritional requirements for LAB growth are strain-dependent (Degeest and De Vuyst 1999; Saguir and de Nadra, 2007), thus, alternative yeast extracts (X-Seed Nucleo Max, KAT, and Carbo Max) in the optimized medium demonstrated promising results as compared to conventional MRS. A basal medium (2 g/l of X-Seed Nucleo Max: N-Max) with KCMax (10/10) i.e.…”

Section: Resultsmentioning

confidence: 99%

Nitrogen source: an effective component for the growth and viability of Lactobacillus delbrueckii subsp. bulgaricus

Ayivi

Ibrahim

Krastanov

et al. 2022

Journal of Dairy Research

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In this study, we developed and optimized a growth media by evaluating various nitrogen sources for the cultivation of Lactobacillus bulgaricus, a probiotic and an important dairy starter culture. We modified the composition of deMan, Rogosa and Sharpe (MRS) culture media and substituted the nitrogen content with alternative nitrogen sources X-Seed KAT, X-Seed Carbo Max and X-Seed Nucleo Max in various blends of 5 g/l and 10 g/l respectively. Results showed that bacterial growth was significantly higher when the nitrogen source blend KCMax (10/10) was used. The optical density (OD610 nm) of the Lactobacillus bulgaricus strains were higher (1.34 and 1.79) in the KCMax (10/10) medium than in the MRS medium (0.89 and 1.42) (P < 0.05). There was no significant difference in the bacterial counts for both the MRS medium and the KCMax (10/10) medium, and all bacterial counts were estimated at 8 log CFU/ml. The buffering capacity of KCMax (10/10) was also tested and supplemented with l-histidine and was significantly different (P < 0.05) than that of the MRS control medium. Calcium supplemented in the KCMax (10/10) also served as a cryoprotectant for the cells during freezing and freeze-drying. Bacterial counts of the recovered calcium-treated freeze-dried cells were statistically significant (P < 0.05). We hypothesized that alternative nitrogen sources such as selected yeast extracts from the X-Seed brand of complex nitrogen sources could efficiently support the viability of Lb. bulgaricus. Our results thus suggested the growth of Lb. bulgaricus was efficiently supported by the X-Seed KAT, X-Seed Nucleo Max and X-Seed Carbo Max nitrogen sources. Consequently, these alternative nitrogen sources could potentially be recommended for dairy starter culture fermentations.

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Edible Growth Medium: A New Window for Probiotic Research

Cited by 6 publications

References 20 publications

Plant Broth- (Not Bovine-) Based Culture Media Provide the Most Compatible Vegan Nutrition for In Vitro Culturing and In Situ Probing of Plant Microbiota

Plant Broth- (Not Bovine-) Based Culture Media Provide the Most Compatible Vegan Nutrition for In Vitro Culturing and In Situ Probing of Plant Microbiota

Production of Postbiotic Metabolites γ-Aminobutyric Acid (GABA) and Bacteriocin-Like Inhibitory Substances (BLIS) by Lactobacillus brevis C23 Co-culture in Coconut-Based Medium for Potential Psychobiotics and Food Preservatives

Nitrogen source: an effective component for the growth and viability of Lactobacillus delbrueckii subsp. bulgaricus

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