Edible Dye-Enhanced Solar Disinfection with Safety Indication

Ryberg, Eric C.; Chu, Chiheng; Kim, Jae Hong

doi:10.1021/acs.est.8b03866

Cited by 29 publications

(24 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Photodynamic inactivation (PDI) can be used to destroy pathogenic microorganisms including bacteria, viruses, fungi, and protozoa [1,2,3] which has practical applications for virus-associated lesions treatment [4] and the disinfection of water [5] or blood products [6]. The PDI technique works by exposing microorganisms to a photosensitizer which, when irradiated with the spectral region corresponding to the photosensitizer absorption bands, converts molecular oxygen into toxic reactive oxygen species (ROS).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Aspects of Photodynamic Inactivation of Highly Pathogenic Avian H5N8 Influenza Virus

Korneev

Kurskaya

Sharshov

et al. 2019

Viruses

View full text Add to dashboard Cite

Ultrastructural studies revealing morphological differences between intact and photodynamically inactivated virions can point to inactivation mechanisms and molecular targets. Using influenza as a model system, we show that photodynamic virus inactivation is possible without total virion destruction. Indeed, irradiation with a relatively low concentration of the photosensitizer (octacationic octakis(cholinyl) zinc phthalocyanine) inactivated viral particles (the virus titer was determined in Madin Darby Canine Kidney (MDCK) cells) but did not destroy them. Transmission electron microscopy (TEM) revealed that virion membranes kept structural integrity but lost their surface glycoproteins. Such structures are known as “bald” virions, which were first described as a result of protease treatment. At a higher photosensitizer concentration, the lipid membranes were also destroyed. Therefore, photodynamic inactivation of influenza virus initially results from surface protein removal, followed by complete virion destruction. This study suggests that photodynamic treatment can be used to manufacture “bald” virions for experimental purposes. Photodynamic inactivation is based on the production of reactive oxygen species which attack and destroy biomolecules. Thus, the results of this study can potentially apply to other enveloped viruses and sources of singlet oxygen.

show abstract

Section: Introductionmentioning

confidence: 99%

Ultrastructural Aspects of Photodynamic Inactivation of Highly Pathogenic Avian H5N8 Influenza Virus

Korneev

Kurskaya

Sharshov

et al. 2019

Viruses

View full text Add to dashboard Cite

show abstract

“…In addition, the color intensity and fading of dye photosensitizers could serve as an indicator of the antimicrobial lifetime of air filtration media. 67 …”

Section: Environmental Implicationsmentioning

confidence: 99%

Photosensitized Electrospun Nanofibrous Filters for Capturing and Killing Airborne Coronaviruses under Visible Light Irradiation

Shen

Zhu

Wang

et al. 2022

Environ. Sci. Technol.

View full text Add to dashboard Cite

To address the challenge of the airborne transmission of SARS-CoV-2, photosensitized electrospun nanofibrous membranes were fabricated to effectively capture and inactivate coronavirus aerosols. With an ultrafine fiber diameter (∼200 nm) and a small pore size (∼1.5 μm), optimized membranes caught 99.2% of the aerosols of the murine hepatitis virus A59 (MHV-A59), a coronavirus surrogate for SARS-CoV-2. In addition, rose bengal was used as the photosensitizer for membranes because of its excellent reactivity in generating virucidal singlet oxygen, and the membranes rapidly inactivated 97.1% of MHV-A59 in virus-laden droplets only after 15 min irradiation of simulated reading light. Singlet oxygen damaged the virus genome and impaired virus binding to host cells, which elucidated the mechanism of disinfection at a molecular level. Membrane robustness was also evaluated, and in general, the performance of virus filtration and disinfection was maintained in artificial saliva and for long-term use. Only sunlight exposure photobleached membranes, reduced singlet oxygen production, and compromised the performance of virus disinfection. In summary, photosensitized electrospun nanofibrous membranes have been developed to capture and kill airborne environmental pathogens under ambient conditions, and they hold promise for broad applications as personal protective equipment and indoor air filters.

show abstract

“…In addition, color intensity and fading of the dye photosensitizers could serve as an indicator of the antimicrobial lifetime of the air filtration media. 49 Future studies should focus on developing robust photoreactive air filters with extended lifetime to overcome the challenges of photobleaching of the dye-sensitized membranes, e.g., replacing the dyes with stable visible-light-responsive photocatalysts. In addition, further elucidating the mechanism of ROS damage to the coronaviruses will provide fundamental insights on designing advanced antimicrobial air filters.…”

Section: Environmental Implicationmentioning

confidence: 99%

Photosensitized Electrospun Nanofibrous Filters for Capturing and Killing Airborne Coronaviruses under Visible Light Irradiation

Shen

Zhu

Wang

et al. 2021

Preprint

View full text Add to dashboard Cite

To address the challenge of the airborne transmission of SARS-CoV-2, photosensitized electrospun nanofibrous membranes were fabricated to effectively capture and inactivate coronavirus aerosols. With an ultrafine fiber diameter (~ 200 nm) and a small pore size (~ 1.5 μm), the optimized membranes caught 99.2% of the aerosols of the murine hepatitis virus A59 (MHV-A59), a coronavirus surrogate for SARS-CoV-2. In addition, rose bengal was used as the photosensitizer for the membranes because of its excellent reactivity in generating virucidal singlet oxygen, and the membranes rapidly inactivated 98.9% of MHV-A59 in virus-laden droplets only after 15 min irradiation of simulated reading light. Singlet oxygen damaged the virus genome and impaired virus binding to host cells, which elucidated the mechanism of disinfection at a molecular level. Membrane robustness was also evaluated, and no efficiency reduction for filtering MHV-A59 aerosols was observed after the membranes being exposed to both indoor light and sunlight for days. Nevertheless, sunlight exposure photobleached the membranes, reduced singlet oxygen production, and compromised the performance of disinfecting MHV-A59 in droplets. In contrast, the membranes after simulated indoor light exposure maintained their excellent disinfection performance. In summary, photosensitized electrospun nanofibrous membranes have been developed to capture and kill airborne environmental pathogens under ambient conditions, and they hold promise for broad applications as personal protective equipment and indoor air filters.

show abstract

Edible Dye-Enhanced Solar Disinfection with Safety Indication

Cited by 29 publications

References 67 publications

Ultrastructural Aspects of Photodynamic Inactivation of Highly Pathogenic Avian H5N8 Influenza Virus

Ultrastructural Aspects of Photodynamic Inactivation of Highly Pathogenic Avian H5N8 Influenza Virus

Photosensitized Electrospun Nanofibrous Filters for Capturing and Killing Airborne Coronaviruses under Visible Light Irradiation

Photosensitized Electrospun Nanofibrous Filters for Capturing and Killing Airborne Coronaviruses under Visible Light Irradiation

Contact Info

Product

Resources

About