Edaphobacter dinghuensis sp. nov., an acidobacterium isolated from lower subtropical forest soil

Abstract

An aerobic bacterium, designated DHF9 T , was isolated from a soil sample collected from the lower subtropical forest of Dinghushan Biosphere Reserve, Guangdong Province, PR China. Cells were Gram-stain-negative, non-motile, short rods that multiplied by binary division. Strain DHF9 T was an obligately acidophilic, mesophilic bacterium capable of growth at pH 3.5-5.5 (optimum pH 4.0) and at 10-33 8C (optimum 28-33 8C). Growth was inhibited at NaCl concentrations above 2.0 % (w/v). The major fatty acids were iso-C 15 : 0 , C 16 : 0 and C 16 : 1 v7c. The polar lipids consist of phosphatidylethanolamine, two unidentified aminolipids, two unidentified phospholipids, two unidentified polar lipids and an unidentified glycolipid. The DNA G+C content was 57.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain belongs to the genus Edaphobacter in subdivision 1 of the phylum Acidobacteria, with the highest 16S rRNA gene sequence similarity of 97.0 % to Edaphobacter modestus Jbg-1 T . Based on phylogenetic, chemotaxonomic and physiological analyses, it is proposed that strain DHF9 T represents a novel species of the genus Edaphobacter, named Edaphobacter dinghuensis sp. nov. The type strain is DHF9 T (5DSM 29920 T 5CGMCC 1.12997 T ).

The first representative of the globally widespread subdivision 6 Acidobacteria, Vicinamibacter silvestris gen. nov., sp. nov., isolated from subtropical savannah soil

“…The eleven genera, Acidobacterium (Kishimoto et al, 1991), Terriglobus (Baik et al, 2013;Eichorst et al, 2007;M€ annistö et al, 2011;Whang et al, 2014;), Edaphobacter (Koch et al, 2008;Wang et al, 2015), Acidicapsa , Granulicella (M€ annistö et al, 2012;Pankratov & Dedysh, 2010;Yamada et al, 2014), Telmatobacter (Pankratov et al, 2012), Acidipila (Jiang et al, 2015;Okamura et al, 2011), Bryocella , Occallatibacter , 'Terracidiphilus' (Garcia-Fraile et al, 2015) and 'Silvibacterium' (Lladó et al, 2016) belong to sd1. The two genera, Bryobacter (Kulichevskaya et al, 2010) and Paludibaculum (Kulichevskaya et al, 2014), fall into sd3.…”

Genome analysis and description of Tunturibacter gen. nov. expands the diversity of Terriglobia in tundra soils

“…Through rRNA operon and full genome analysis, we expand the current phylogenetic diversity of the order Terriglobales in the Acidobacteriota. We also delineate and name a novel genus, Tunturibacter, containing four new species in phylogenetic proximity to other species within the genera Terriglobus (Eichorst et al, 2007;Männistö et al, 2011;Podar et al, 2019;Rawat et al, 2012), Granulicella (Männistö et al, 2012;Pankratov & Dedysh, 2010;Rawat et al, 2013Rawat et al, , 2014, and Edaphobacter (Koch et al, 2008;Wang et al, 2016). With functional annotation and comparison of this new genus to known Acidobacteriota, the core and unique genes of the genera within this group can be identified.…”