Ectothiorhodospira variabilis sp. nov., an alkaliphilic and halophilic purple sulfur bacterium from soda lakes

Горленко, В. М.; Bryantseva, I. A.; Rabold, Sandra; Tourova, T. P.; Rubtsova, Dariya; Смирнова, Е В; Thiel, Vera; Imhoff, Johannes F.

doi:10.1099/ijs.0.004648-0

Cited by 39 publications

(14 citation statements)

References 25 publications

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“…Ninety‐four cloned 16S rRNA gene amplicons from an early transfer of EC8 were sequenced yielding 83 usable sequences. Forty‐six (55.4%) of these shared > 99% identity to the Ectothiorhodospira variabilis strain WN22 (Gorlenko et al ., ) 16S rRNA gene (). The remaining sequences consisted of Firmicutes (28, 33.7%), Bacteroidetes (8, 9.6%) and Spirochetes (1, 1.2%).…”

Section: Resultsmentioning

confidence: 99%

Transformation of monothioarsenate by haloalkaliphilic, anoxygenic photosynthetic purple sulfur bacteria

Edwardson

Planer-Friedrich

Hollibaugh

2014

FEMS Microbiol Ecol

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Thioarsenates are the dominant arsenic species in arsenic-rich, alkaline, and sulfidic waters, but bacterial interactions with these compounds have only recently been examined. Previous studies have shown that microorganisms play a role in the transformation of monothioarsenate to arsenate, including use of monothioarsenate as a chemolithotrophic electron donor coupled with oxygen as an electron acceptor. We obtained enrichment cultures from two saline, alkaline lakes (Mono Lake, CA and Big Soda Lake, NV) that are able to use monothioarsenate as the sole electron donor for anoxygenic photosynthesis. These anoxic cultures were able to convert a 1 mM mixture of thioarsenates completely to arsenate in c. 13 days and 4 mM monothioarsenate to arsenate in c. 17 days. This conversion was light dependent; thus, monothioarsenate can be used as the sole electron donor for anoxygenic photosynthesis. Both of the Mono Lake and Big Soda Lake enrichment cultures were dominated by an organism closely related to Ectothiorhodospira species. We tested additional strains of purple sulfur bacteria and found widespread ability to use monothioarsenate as an electron donor. The ability of bacteria to transform thioarsenates directly via anoxygenic photosynthesis adds a new perspective to the well-studied arsenic and sulfur cycles.

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Section: Resultsmentioning

confidence: 99%

Transformation of monothioarsenate by haloalkaliphilic, anoxygenic photosynthetic purple sulfur bacteria

Edwardson

Planer-Friedrich

Hollibaugh

2014

FEMS Microbiol Ecol

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“…Our agricultural clone libraries did not suggest an abundance of nitrite-oxidizing Nitrospira and phototrophic Cyanobacteria in the soil, a few sequences were identified and more may be present because the rarefaction curves (Additional file 6: Figure S4b) did not reach an asymptote. The Gammaproteobacteria sequences in SS2 clone library were related to the phototrophic Ectothiorhodospira , an alkaliphilic and halophilic purple sulphur bacterium from soda lake [52]. The phylotype HSS148 was distantly related (88%) to the chemolithotroph Thioalkalivibrio , which oxidizes sulphide or thiosulphate with molecular oxygen.…”

Section: Discussionmentioning

confidence: 99%

Comparative molecular analysis of chemolithoautotrophic bacterial diversity and community structure from coastal saline soils, Gujarat, India

et al. 2012

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BackgroundSoils harbour high diversity of obligate as well as facultative chemolithoautotrophic bacteria that contribute significantly to CO2 dynamics in soil. In this study, we used culture dependent and independent methods to assess the community structure and diversity of chemolithoautotrophs in agricultural and coastal barren saline soils (low and high salinity). We studied the composition and distribution of chemolithoautotrophs by means of functional marker gene cbbL encoding large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase and a phylogenetic marker 16S rRNA gene. The cbbL form IA and IC genes associated with carbon fixation were analyzed to gain insight into metabolic potential of chemolithoautotrophs in three soil types of coastal ecosystems which had a very different salt load and sulphur content.ResultsIn cbbL libraries, the cbbL form IA was retrieved only from high saline soil whereas form IC was found in all three soil types. The form IC cbbL was also amplified from bacterial isolates obtained from all soil types. A number of novel monophyletic lineages affiliated with form IA and IC phylogenetic trees were found. These were distantly related to the known cbbL sequences from agroecosystem, volcanic ashes and marine environments. In 16S rRNA clone libraries, the agricultural soil was dominated by chemolithoautotrophs (Betaproteobacteria) whereas photoautotrophic Chloroflexi and sulphide oxidizers dominated saline ecosystems. Environmental specificity was apparently visible at both higher taxonomic levels (phylum) and lower taxonomic levels (genus and species). The differentiation in community structure and diversity in three soil ecosystems was supported by LIBSHUFF (P = 0.001) and UniFrac.ConclusionThis study may provide fundamentally new insights into the role of chemolithoautotrophic and photoautotrophic bacterial diversity in biochemical carbon cycling in barren saline soils. The bacterial communities varied greatly among the three sites, probably because of differences in salinity, carbon and sulphur contents. The cbbL form IA-containing sulphide-oxidizing chemolithotrophs were found only in high saline soil clone library, thus giving the indication of sulphide availability in this soil ecosystem. This is the first comparative study of the community structure and diversity of chemolithoautotrophic bacteria in coastal agricultural and saline barren soils using functional (cbbL) and phylogenetic (16S rDNA) marker genes.

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“…The communities of purple sulfur bacteria from both salt lakes were characterized by the presence of representatives related to the type strains of moderately and extremely halophilic Chromatiaceae such as Halochromatium salexigens , Halochromatium glycolicum , Thiohalocapsa halophila, Ectothiorhodospira mobilis, Ectothiorhodospira variabilis and Halorhodospira halophila as closest relatives [ 76 , 77 , 78 , 83 ]. Evidence was also obtained for the presence of several phylotypes of BChl b -containing anoxygenic phototrophic bacteria distant to (<80% sequence similarity) the genera Thiococcus , Thioflavicoccus and Thioalkalicoccus , that form a separate phylogenetic branch among the purple sulfur bacteria [ 48 , 52 , 84 ].…”

Section: Photosynthesis and Anoxygenic Phototrophic Bacteriamentioning

confidence: 99%

New Dimensions in Microbial Ecology—Functional Genes in Studies to Unravel the Biodiversity and Role of Functional Microbial Groups in the Environment

Imhoff

2016

Microorganisms

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During the past decades, tremendous advances have been made in the possibilities to study the diversity of microbial communities in the environment. The development of methods to study these communities on the basis of 16S rRNA gene sequences analysis was a first step into the molecular analysis of environmental communities and the study of biodiversity in natural habitats. A new dimension in this field was reached with the introduction of functional genes of ecological importance and the establishment of genetic tools to study the diversity of functional microbial groups and their responses to environmental factors. Functional gene approaches are excellent tools to study the diversity of a particular function and to demonstrate changes in the composition of prokaryote communities contributing to this function. The phylogeny of many functional genes largely correlates with that of the 16S rRNA gene, and microbial species may be identified on the basis of functional gene sequences. Functional genes are perfectly suited to link culture-based microbiological work with environmental molecular genetic studies. In this review, the development of functional gene studies in environmental microbiology is highlighted with examples of genes relevant for important ecophysiological functions. Examples are presented for bacterial photosynthesis and two types of anoxygenic phototrophic bacteria, with genes of the Fenna-Matthews-Olson-protein (fmoA) as target for the green sulfur bacteria and of two reaction center proteins (pufLM) for the phototrophic purple bacteria, with genes of adenosine-5′phosphosulfate (APS) reductase (aprA), sulfate thioesterase (soxB) and dissimilatory sulfite reductase (dsrAB) for sulfur oxidizing and sulfate reducing bacteria, with genes of ammonia monooxygenase (amoA) for nitrifying/ammonia-oxidizing bacteria, with genes of particulate nitrate reductase and nitrite reductases (narH/G, nirS, nirK) for denitrifying bacteria and with genes of methane monooxygenase (pmoA) for methane oxidizing bacteria.

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Ectothiorhodospira variabilis sp. nov., an alkaliphilic and halophilic purple sulfur bacterium from soda lakes

Cited by 39 publications

References 25 publications

Transformation of monothioarsenate by haloalkaliphilic, anoxygenic photosynthetic purple sulfur bacteria

Transformation of monothioarsenate by haloalkaliphilic, anoxygenic photosynthetic purple sulfur bacteria

Comparative molecular analysis of chemolithoautotrophic bacterial diversity and community structure from coastal saline soils, Gujarat, India

New Dimensions in Microbial Ecology—Functional Genes in Studies to Unravel the Biodiversity and Role of Functional Microbial Groups in the Environment

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