Ectopic Recombination between Ty Elements in <i>Saccharomyces cerevisiae</i> Is Not Induced by DNA Damage

Parket, Anat; Kupiec, Martin

doi:10.1128/mcb.12.10.4441-4448.1992

Molecular and Cellular Biology

1992

DOI: 10.1128/mcb.12.10.4441-4448.1992

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Ectopic Recombination between Ty Elements in Saccharomyces cerevisiae Is Not Induced by DNA Damage

Anat Parket

Martin Kupiec

Abstract: Mitotic recombination is increased when cells are treated with a variety of physical and chemical agents that cause damage to their DNA. We show here, using Saccharomyces cerevisiae strains that carry marked Ty elements, that recombination between members of this family of retrotransposons is not increased by UV irradiation or by treatment with the radiomimetic drug methyl methanesulfonate. Both ectopic recombination and mutation events were elevated by these agents for non-Ty sequences in the same strain. We … Show more

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Cited by 17 publications

References 42 publications

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Analysis of a Ty1‐less variant of Saccharomyces paradoxus: the gain and loss of Ty1 elements

Moore¹,

Liti²,

Stefanisko³

et al. 2004

Yeast

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Because Ty elements transpose through an RNA intermediate, element accumulation through retrotransposition must be regulated or offset by element loss to avoid uncontrolled genome expansion. Here we examine the fate of Ty sequences in Saccharomyces strain 337, a strain that is reported to lack Ty1 and Ty2 elements, but contains remnant solo long terminal repeats (LTRs). Although strain 337 was initially classified as Saccharomyces cerevisiae, our work indicates that this strain is more closely related to S. paradoxus. Several degenerate Ty1 and Ty2 LTRs were mapped to the same insertion sites as full-length Ty1 and Ty2 elements in S. cerevisiae, suggesting that this strain lost Ty elements by LTR-LTR recombination. Southern analysis indicates that strain 337 also lacks Ty4 and Ty5 elements. We estimated the rates of element gain and loss in this strain by introducing a single transpositioncompetent Ty1 element. The results indicate that Ty1 retrotransposition occurs at a much higher rate than elimination, suggesting that copy-number-dependent cofactors or environmental conditions contribute to the loss of Ty elements in this genome.

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Analysis of a Ty1‐less variant of Saccharomyces paradoxus: the gain and loss of Ty1 elements

Moore¹,

Liti²,

Stefanisko³

et al. 2004

Yeast

View full text Add to dashboard Cite

show abstract

Mating type regulates the radiation-associated stimulation of reciprocal translocation events in Saccharomyces cerevisiae

Fasullo

Dave

1994

Molec. Gen. Genet.

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Both ultraviolet (UV) and ionizing radiation were observed to stimulate mitotic, ectopic recombination between his3 recombinational substrates, generating reciprocal translocations in Saccharomyces cerevisiae (yeast). The stimulation was greatest in diploid strains competent for sporulation and depends upon both the ploidy of the strain and heterozygosity at the MATlocus. The difference in levels of stimulation between MATa/MAT alpha diploid and MAT alpha haploid strains increases when cells are exposed to higher levels of UV radiation (sevenfold at 150 J/m2), whereas when cells are exposed to higher levels of ionizing radiation (23.4 krad), only a twofold difference is observed. When the MAT alpha gene was introduced by DNA transformation into a MATa/mat alpha::LEU2+ diploid, the levels of radiation-induced ectopic recombination approach those obtained in a strain that is heterozygous at MAT. Conversely, when the MATa gene was introduced by DNA transformation into a MAT alpha haploid, no enhanced stimulation of ectopic recombination was observed when cells were irradiated with ionizing radiation but a threefold enhancement was observed when cells were irradiated with UV. The increase in radiation-stimulated ectopic recombination resulting from heterozygosity at MAT correlated with greater spontaneous ectopic recombination and higher levels of viability after irradiation. We suggest that MAT functions that have been previously shown to control the level of mitotic, allelic recombination (homolog recombination) also control the level of mitotic, radiation-stimulated ectopic recombination between short dispersed repetitive sequences on non-homologous chromosomes.

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Efficient UV stimulation of yeast integrative transformation requires damage on both plasmid strands

et al. 1994

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The nature of UV-induced pre-recombinational structures was studied using transformation of Saccharomyces cerevisiae cells with non-replicative plasmids. Transformation by double-stranded plasmids irradiated with UV was stimulated up to 50-fold, and both plasmid integration and conversion of the mutated chromosomal selective gene were found to be equally increased. The stimulation observed with such 'totally' irradiated plasmids was not found with plasmids bearing lesions in only one strand. This effect is attributed to the formation by excision repair of recombinogenic structures consisting of a pyrimidine dimer opposite a gap. When single-stranded integrative plasmids were irradiated, their transforming potential was decreased but the proportion of transformants that arose by gene conversion, rather than by plasmid integration, was increased from 8% to 49% as a function of the UV dose. Possible reasons why single-strand UV lesions favour gene conversion are discussed.

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Ectopic Recombination between Ty Elements in Saccharomyces cerevisiae Is Not Induced by DNA Damage

Cited by 17 publications

References 42 publications

Analysis of a Ty1‐less variant of Saccharomyces paradoxus: the gain and loss of Ty1 elements

Analysis of a Ty1‐less variant of Saccharomyces paradoxus: the gain and loss of Ty1 elements

Mating type regulates the radiation-associated stimulation of reciprocal translocation events in Saccharomyces cerevisiae

Efficient UV stimulation of yeast integrative transformation requires damage on both plasmid strands

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