Ectopic overexpression of the aldehyde dehydrogenase ALDH21 from Syntrichia caninervis in tobacco confers salt and drought stress tolerance

Hong-yuan, Yang; Zhang, Daoyuan; Li, Haiyan; Dong, Lu; Lan, Haiyan

doi:10.1016/j.plaphy.2015.07.001

Cited by 41 publications

(33 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some ALDH subfamilies have been shown to play important roles in plant resistance to biotic and abiotic stressors (Liu et al, 2001;Sophos et al, 2001;Singh et al, 2013) and one unique subfamily, ALDH21, exists in mosses and has been indicated to respond to abiotic stress at the transcript abundance level (Chen et al, 2002;Kirch et al, 2004;Wood and Oliver, 2004;Wood and Duff 2009;Yang et al, 2012). In our previous work, we cloned the ALDH21 gene from Syntrichia caninervis, a desiccation-tolerant desert moss, and validated its enhancement of abiotic stress ability by constitutive overexpression in cotton (and tobacco) under controlled conditions (Yang et al, 2015c(Yang et al, , 2016. Until now, its growth behavior under deficit watering conditions and its potential ability to improve cotton yield and fiber quality in production field conditions were not well studied, which greatly limited future gene applications.…”

Section: Discussionmentioning

confidence: 99%

“…when overexpressed in tobacco (Nicoiana tabacum L.) and Gossypium hirsutum L. (Yang et al, 2015c(Yang et al, , 2016 can improve vegetative salt and drought tolerance under greenhouse and field conditions. when overexpressed in tobacco (Nicoiana tabacum L.) and Gossypium hirsutum L. (Yang et al, 2015c(Yang et al, , 2016 can improve vegetative salt and drought tolerance under greenhouse and field conditions.…”

Section: Overexpression Of Aldh21 From Syntrichia Caninervis Moss In mentioning

confidence: 99%

“…In our previous studies, we demonstrated that aldehyde dehydrogenases gene ScALDH21 from the desiccation-tolerant desert moss Syntrichia caninervis Mitt. when overexpressed in tobacco (Nicoiana tabacum L.) and Gossypium hirsutum L. (Yang et al, 2015c(Yang et al, , 2016 can improve vegetative salt and drought tolerance under greenhouse and field conditions. However, to be an effective transgenic approach, it is necessary to determine how the constitutive overexpression of ScALDH21 affects cotton yields and fiber quality under both full and deficit irrigations.…”

Section: Overexpression Of Aldh21 From Syntrichia Caninervis Moss In mentioning

confidence: 99%

See 2 more Smart Citations

Overexpression of ALDH21 from Syntrichia caninervis Moss in Upland Cotton Enhances Fiber Quality, Boll Component Traits, and Physiological Parameters during Deficit Irrigation

et al. 2019

Self Cite

View full text Add to dashboard Cite

Plants have evolved complex molecular, cellular, and physiological mechanisms to respond to environmental stressors. Genetic manipulationhas represented an important potential method for improving water deficit tolerance in crops. Aldehyde dehydrogenases are involved in cellular responses to oxidative stress to protect against a variety of environmental stressors. The Syntrichia caninervis Mitt. ALDH21 gene plays a role in plant responses to abiotic stresses, and overexpression of this gene in tobacco (Nicoiana tabacum L.) and cotton (Gossypium hirsutum L.) decreases their sensitivity that improves tolerance to drought and salt stresses. To test the possibility that transgenic cotton constitutively expressing ScALDH21 may be suitable for cultivating under water deficit conditions, phenotype, physiological response, and yield of transgenic ScALDH21 cotton were measured in managed treatment plots and under field conditions. Overexpression of ScALDH21 in cotton resulted in higher net photosynthetic rate, less cellular damage, more cellular protective compounds, and enhanced growth compared with nontransgenic (NT) cotton under drought stress in managed treatment plots. Yield of transgenic cottons under deficit irrigation condition was increased above that for NT plants measured under full irrigation conditions. Under field conditions, transgenic cotton yield increased ∼10.0% under full irrigation and ∼18.0% under deficit irrigation conditions compared with NT. Fiber quality of transgenic cotton lines was also improved compared with NT under both full and deficit irrigation. These results suggest that transgenic ScALDH21 cotton is a viable candidate material for improving crop yields in water‐limited agricultural production systems.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Overexpression Of Aldh21 From Syntrichia Caninervis Moss In mentioning

confidence: 99%

Section: Overexpression Of Aldh21 From Syntrichia Caninervis Moss In mentioning

confidence: 99%

See 1 more Smart Citation

Overexpression of ALDH21 from Syntrichia caninervis Moss in Upland Cotton Enhances Fiber Quality, Boll Component Traits, and Physiological Parameters during Deficit Irrigation

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Meanwhile, proline may act as a signaling/regulatory molecule to activate multiple adaptive responses [54]. The overexpression of an aldehyde dehydrogenase gene ALDH21 in tobacco increased the proline accumulation in transgenic plants, and enhanced drought and salt tolerance [55]. In addition, 22 co-functional genes of GmALDHs are related to response to water deprivation/water transport (Additional file 10: Table S5).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide characterization of the aldehyde dehydrogenase gene superfamily in soybean and its potential role in drought stress response

Wang¹,

Liu²,

Li³

et al. 2017

BMC Genomics

View full text Add to dashboard Cite

BackgroundAldehyde dehydrogenases (ALDHs) represent a group of enzymes that detoxify aldehydes by facilitating their oxidation to carboxylic acids, and have been shown to play roles in plant response to abiotic stresses. However, the comprehensive analysis of ALDH superfamily in soybean (Glycine max) has been limited.ResultsIn present study, a total of 53 GmALDHs were identified in soybean, and grouped into 10 ALDH families according to the ALDH Gene Nomenclature Committee and phylogenetic analysis. These groupings were supported by their gene structures and conserved motifs. Soybean ALDH superfamily expanded mainly by whole genome duplication/segmental duplications. Gene network analysis identified 1146 putative co-functional genes of 51 GmALDHs. Gene Ontology (GO) enrichment analysis suggested the co-functional genes of these 51 GmALDHs were enriched (FDR < 1e-3) in the process of lipid metabolism, photosynthesis, proline catabolism, and small molecule catabolism. In addition, 22 co-functional genes of GmALDHs are related to plant response to water deprivation/water transport. GmALDHs exhibited various expression patterns in different soybean tissues. The expression levels of 13 GmALDHs were significantly up-regulated and 14 down-regulated in response to water deficit. The occurrence frequencies of three drought-responsive cis-elements (ABRE, CRT/DRE, and GTGCnTGC/G) were compared in GmALDH genes that were up-, down-, or non-regulated by water deficit. Higher frequency of these three cis-elements was observed for the group of up-regulated GmALDH genes as compared to the group of down- or non- regulated GmALDHs by drought stress, implying their potential roles in the regulation of soybean response to drought stress.ConclusionsA total of 53 ALDH genes were identified in soybean genome and their phylogenetic relationships and duplication patterns were analyzed. The potential functions of GmALDHs were predicted by analyses of their co-functional gene networks, gene expression profiles, and cis-regulatory elements. Three GmALDH genes, including GmALDH3H2, GmALDH12A2 and GmALDH18B3, were highly induced by drought stress in soybean leaves. Our study provides a foundation for future investigations of GmALDH gene function in soybean.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3908-y) contains supplementary material, which is available to authorized users.

show abstract

“…The expression of ALDH21 gene is strongly induced by a desiccation and turns back upon rehydration in the twisted mosses Syntrichia (Tortula) ruralis and Syntrichia caninervis (Chen et al ., ; Yang et al ., ). Recent studies have shown that the overexpression of ALDH21 in cotton and tobacco improves their drought and salt tolerance (Yang et al ., , ). However, function of the enzyme as well as the reaction metabolites have not been reported so far.…”

Section: Introductionmentioning

confidence: 99%

The ALDH21 gene found in lower plants and some vascular plants codes for a NADP⁺‐dependent succinic semialdehyde dehydrogenase

et al. 2017

View full text Add to dashboard Cite

Lower plant species including some green algae, non-vascular plants (bryophytes) as well as the oldest vascular plants (lycopods) and ferns (monilophytes) possess a unique aldehyde dehydrogenase (ALDH) gene named ALDH21, which is upregulated during dehydration. However, the gene is absent in flowering plants. Here, we show that ALDH21 from the moss Physcomitrella patens codes for a tetrameric NADP -dependent succinic semialdehyde dehydrogenase (SSALDH), which converts succinic semialdehyde, an intermediate of the γ-aminobutyric acid (GABA) shunt pathway, into succinate in the cytosol. NAD is a very poor coenzyme for ALDH21 unlike for mitochondrial SSALDHs (ALDH5), which are the closest related ALDH members. Structural comparison between the apoform and the coenzyme complex reveal that NADP binding induces a conformational change of the loop carrying Arg-228, which seals the NADP in the coenzyme cavity via its 2'-phosphate and α-phosphate groups. The crystal structure with the bound product succinate shows that its carboxylate group establishes salt bridges with both Arg-121 and Arg-457, and a hydrogen bond with Tyr-296. While both arginine residues are pre-formed for substrate/product binding, Tyr-296 moves by more than 1 Å. Both R121A and R457A variants are almost inactive, demonstrating a key role of each arginine in catalysis. Our study implies that bryophytes but presumably also some green algae, lycopods and ferns, which carry both ALDH21 and ALDH5 genes, can oxidize SSAL to succinate in both cytosol and mitochondria, indicating a more diverse GABA shunt pathway compared with higher plants carrying only the mitochondrial ALDH5.

show abstract

Ectopic overexpression of the aldehyde dehydrogenase ALDH21 from Syntrichia caninervis in tobacco confers salt and drought stress tolerance

Cited by 41 publications

References 38 publications

Overexpression of ALDH21 from Syntrichia caninervis Moss in Upland Cotton Enhances Fiber Quality, Boll Component Traits, and Physiological Parameters during Deficit Irrigation

Overexpression of ALDH21 from Syntrichia caninervis Moss in Upland Cotton Enhances Fiber Quality, Boll Component Traits, and Physiological Parameters during Deficit Irrigation

Genome-wide characterization of the aldehyde dehydrogenase gene superfamily in soybean and its potential role in drought stress response

The ALDH21 gene found in lower plants and some vascular plants codes for a NADP⁺‐dependent succinic semialdehyde dehydrogenase

Contact Info

Product

Resources

About

Ectopic overexpression of the aldehyde dehydrogenase ALDH21 from Syntrichia caninervis in tobacco confers salt and drought stress tolerance

Cited by 41 publications

References 38 publications

Overexpression of ALDH21 from Syntrichia caninervis Moss in Upland Cotton Enhances Fiber Quality, Boll Component Traits, and Physiological Parameters during Deficit Irrigation

Overexpression of ALDH21 from Syntrichia caninervis Moss in Upland Cotton Enhances Fiber Quality, Boll Component Traits, and Physiological Parameters during Deficit Irrigation

Genome-wide characterization of the aldehyde dehydrogenase gene superfamily in soybean and its potential role in drought stress response

The ALDH21 gene found in lower plants and some vascular plants codes for a NADP+‐dependent succinic semialdehyde dehydrogenase

Contact Info

Product

Resources

About

The ALDH21 gene found in lower plants and some vascular plants codes for a NADP⁺‐dependent succinic semialdehyde dehydrogenase