Ectopic overexpression of MucACP- and #916;9 desaturase leads to  and #969;-7 fatty acid accumulation in tobacco leaves

Gao, Changyong; Xue, Ming; Shang, H.S.; Ji, Xiajie; Li, Runzhi

doi:10.9755/ejfa.ejfa-2016-10-1356

Cited by 1 publication

(1 citation statement)

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“…These data indicate that constitutive expression of genes that are specifically expressed in seeds in wild-type plants can result in lipid accumulation in vegetative organs. Recently, metabolic engineering has been used to produce tobacco lines with leaf-oil contents enriched in particular fatty acids 30 . These results suggest that additional gene stacking, construct optimization and further refinement of the current metabolic engineering strategies have the potential to yield unprecedented levels of storage lipids in non-seed tissues of a high biomass crop, and that fatty acid profiles could be simultaneously tailored to meet specific market needs, including biofuel, food, feed and biomaterials.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Oil Accumulation in Tobacco (Nicotiana tabacum L.) Leaves by Ectopic Overexpression of VgDGAT1a for Renewable Production of Biofuels

et al. 2018

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To increase oil accumulation in the high-biomass vegetative organs of tobacco (Nicotiana tabacum L.) plants for renewable production of biofuels, VgDGAT1a isolated from developing seeds of Vernonia galamensis L. was ectopically overexpressed in tobacco leaves using a constitutive promoter. The transgenic tobacco leaves showed a 3.5-5.0-fold increase in oil content compared to the control, with a maximum increase of 9.2% (DW). The transgenic leaves also showed a substantial change in fatty acid composition, with significant enhancement of linoleic acid (18 : 2) and notable reduction of -linolenic acid (18 : 3). The overexpression of VgDGAT1a exhibited no deleterious effect on other phenotypes in the tobacco plant. These results will facilitate development of a novel system for lipid metabolic engineering in vegetative organs of plants, as well as provide a platform for the production of biofuels using the vegetative organs of commercial non-food crops.

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Section: Discussionmentioning

confidence: 99%