Ectopic overexpression of maize heat shock transcription factor gene ZmHsf04 confers increased thermo and salt-stress tolerance in transgenic Arabidopsis

Jiang, Yingli; Qin, Zheng; Chen, Long; Liang, Yani; Wu, Jiandong

doi:10.1007/s11738-017-2587-2

Cited by 50 publications

(33 citation statements)

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“…This change trend of expression levels and these typical characteristics in ZmHsf01 was same to the other two reported ZmHsfA2s (Li et al, 2019;Jiang et al, 2018). The conservative domain among these HsfA members means the similarity of gene function.…”

Section: Discussionsupporting

confidence: 86%

“…Among subclass A2, ZmHsf04 and ZmHsf05 were cloned. Ectopic overexpression ZmHsf04 and ZmHsf05 improves the thermotolerance in transgenic Arabidopsis seedlings (Li et al, 2019;Jiang et al, 2018). In our study, ZmHsf01, another member of ZmHsfA2, was cloned and analyzed.…”

Section: Functional Characterization Of a New Maize Heat Shock Transcmentioning

confidence: 88%

“…The Hsf/Hsp network which includes activating downstream responses and feedback suppress the Hsfs played a vital role in HS and other stresses (Frangkostefanakis et al, 2015). In ZmHsf04 and Zmhsf05 transgenic Arabidopsis, the expression levels of all detected AtHsps were higher than that in WT after HS (Li et al, 2019;Jiang et al, 2018). After heat treatment, the transcript levels of some Hsps in ZmHsf01 over-expressed lines are higher than that of WT to some extent, such as AtHsp18.2, AtHsp21, AtHsp70b, AtHsp70T, AtHsp90 and AtHsp101.…”

Section: Discussionmentioning

confidence: 99%

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Supplemental Information 5: The Uncropped Blot of Semi qRT-PCR Result of the ZmHsf01 Transcript in Different Over-Expres

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Section: Discussionsupporting

confidence: 86%

Section: Functional Characterization Of a New Maize Heat Shock Transcmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

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Supplemental Information 5: The Uncropped Blot of Semi qRT-PCR Result of the ZmHsf01 Transcript in Different Over-Expres

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“…288 reported ZmHsfA2s Jiang et al, 2018). The conservative domain among these HsfA members 289 means the similarity of gene function.…”

mentioning

confidence: 99%

“…The Hsf/Hsp network which 307 includes activating downstream responses and feedback suppress the Hsfs played a vital role in HS and other 308 stresses (Frangkostefanakis et al, 2015). In ZmHsf04 and Zmhsf05 transgenic Arabidopsis, the expression levels of all 309 detected AtHsps were higher than that in WT after HS Jiang et al, 2018). After heat treatment, the 310 transcript levels of some Hsps in ZmHsf01 over-expressed lines are higher than that of WT to some extent, such as 311 AtHsp18.2, AtHsp21, AtHsp70b, AtHsp70T, AtHsp90 and AtHsp101.…”

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Supplemental Information 3: The Expression Levels of Heat-Related HSP Genes in WT and ZmHsf01 Over-Expressing Lines 36_7

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Background. The yield of maize crop is influenced seriously by heat waves. Plant heat shock transcription factors (Hsfs) play a key regulatory role in heat shock signal transduction pathway. Method. In this study, a new heat shock transcription factor gene, ZmHsf01 (accession number: MK888854) , was cloned from maize young leaves using homologous cloning method. The transcriptional level of ZmHsf01 were detected by qRT-PCR in different tissues or under heat shock, abscisic acid (ABA) and hydrogen peroxide (H 2 O 2 ) treatment. The transgenic yeast and Arabidopsis were used to study the gene function of ZmHsf01. Result. The coding sequence (CDS) of ZmHsf01 was 1176 bp and encoded a protein that consisted of 391 amino acids. The homologous analysis result showed that ZmHsf01 and SbHsfA2d had the highest protein sequence identity.Subcellular localization experiments demonstrated that ZmHsf01 is localized to the nucleus. ZmHsf01 was expressed in many maize tissues and was up-regulated by heat stress. ZmHsf01 was up-regulated in roots and down-regulated in leaves by ABA and H 2 O 2 treatments. In yeast, ZmHsf01-overexpressing cells showed increased thermotolerance. In Arabidopsis seedlings, ZmHsf01 complemented the thermotolerance defects of athsfa2 mutant and ZmHsf01-overexpressing lines presented enhanced basal and acquired thermotolerance. Compared to wild type (WT) seedlings, ZmHsf01overexpressing lines showed increased chlorophyll content after heat stress. The expression level of heat shock protein genes was up-regulated higher in ZmHsf01overexpressing Arabidopsis seedlings than that in WT. These results suggested that ZmHsf01 plays a vital role in plant response to heat stress. PeerJ Preprints | https://doi.org/10.7287/peerj.preprints.27987v1 | CC BY 4.0 Open Access | rec21 Abbreviations 22 Hsfs, heat shock transcription factors; HSE, heat shock element; HS, heat shock; HSPs, heat shock proteins; RH, 23 relative humidity; CDS, coding sequence; DBD, DNA-binding domain; OD, oligomerization domain; NLS, nuclear 24 localization signal; NES, nuclear export signal; AHA motifs, C-terminal activator motifs; ABA, abscisic acid; H 2 O 2 , 25 hydrogen peroxide; GFP, green fluorescence protein; WT, wild type; BT, basal thermotolerance; AT, acquired 26 thermotolerance. 27 28 Abstract 29 Background. The yield of maize crop is influenced seriously by heat waves. Plant heat shock transcription 30 factors (Hsfs) play a key regulatory role in heat shock signal transduction pathway. 31Method. In this study, a new heat shock transcription factor gene, ZmHsf01(accession number: MK888854), was 32 cloned from maize young leaves using homologous cloning method. The transcriptional level of ZmHsf01 were 33 detected by qRT-PCR in different tissues or under heat shock, abscisic acid (ABA) and hydrogen peroxide (H 2 O 2 ) 34 treatment. The transgenic yeast and Arabidopsis were used to study the gene function of ZmHsf01. 35Result. The coding sequence (CDS) of ZmHsf01 was 1176 bp and encoded a protein that consisted of 391 amino 36 acids. T...

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Biotechnology Strategies to Combat Plant Abiotic Stress

Jalil

Ansari

2021

Nanobiotechnology

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Ectopic overexpression of maize heat shock transcription factor gene ZmHsf04 confers increased thermo and salt-stress tolerance in transgenic Arabidopsis

Cited by 50 publications

References 47 publications

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Biotechnology Strategies to Combat Plant Abiotic Stress

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