Ectopic G-protein expression in dopamine and serotonin neurons blocks cocaine sensitization in Drosophila melanogaster

Li, H.; Chaney, S.; Forte, Michael; Hirsh, Jay

doi:10.1016/s0960-9822(00)00501-7

Cited by 74 publications

(114 citation statements)

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“…The construct was generated from a full-length dSTIM cDNA clone (BDGP LD45776). The other strains used are as follows: Dilp2GAL4, provided by Dr. E. Rulifson (Rulifson et al, 2002), embryonic wild-type itpr cDNA (UASitpr ϩ ) (Venkatesh et al, 2001), GAL80 ts with two inserts on the second chromosome (generated by Albert Chiang, NCBS, Bangalore, India), UASdOrai ϩ (Venkiteswaran and Hasan, 2009), DdcGAL4 referred in the text as aminergic (Li et al, 2000), a recombinant of Dilp2GAL4 and DdcGAL4 referred to as doubleGAL4 and UASmAChR (Venkiteswaran and Hasan, 2009). Elav C155 GAL4 (referred in the text as pan-neuronal), UASdicer(III) and UASmCD8GFP(II) were obtained from the Bloomington Stock Centre, Bloomington, IN.…”

Section: Methodsmentioning

confidence: 99%

Inositol 1,4,5-Trisphosphate Receptor and dSTIM Function inDrosophilaInsulin-Producing Neurons Regulates Systemic Intracellular Calcium Homeostasis and Flight

Agrawal¹,

Venkiteswaran²,

Sadaf³

et al. 2010

J. Neurosci.

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Section: Methodsmentioning

confidence: 99%

Inositol 1,4,5-Trisphosphate Receptor and dSTIM Function inDrosophilaInsulin-Producing Neurons Regulates Systemic Intracellular Calcium Homeostasis and Flight

Agrawal¹,

Venkiteswaran²,

Sadaf³

et al. 2010

J. Neurosci.

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“…2C). The differences in the expression patterns of these GAL4 drivers are in (i) neurons producing serotonin (but not dopamine) and (ii) in most of the dopaminergic PAM neurons, which express Ddc-GAL4 but not TH-GAL4 (31,32). Thus, our data lead to two alternate hypotheses: (i) dopamine suppresses oviposition preference, whereas serotonin enhances it; or (ii) nonoverlapping sets of dopaminergic neurons within the Ddc-and TH-GAL4 patterns affect oviposition preference in opposite ways.…”

Section: Oviposition Preference Depends On the Context Of The Egg-layingmentioning

confidence: 99%

“…TRH-GAL4 expresses in nearly all serotonergic neurons including those expressing Ddc-GAL4, but not in dopaminergic neurons defined by TH-GAL4 expression (27,31,34). If serotonin were required for oviposition preference for ethanol, we expected that blocking TRH neurons would suppress the response.…”

Section: Oviposition Preference Depends On the Context Of The Egg-layingmentioning

confidence: 99%

Competing dopamine neurons drive oviposition choice for ethanol in Drosophila

Azanchi

Kaun

Heberlein

2013

Proc. Natl. Acad. Sci. U.S.A.

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The neural circuits that mediate behavioral choice evaluate and integrate information from the environment with internal demands and then initiate a behavioral response. Even circuits that support simple decisions remain poorly understood. In Drosophila melanogaster, oviposition on a substrate containing ethanol enhances fitness; however, little is known about the neural mechanisms mediating this important choice behavior. Here, we characterize the neural modulation of this simple choice and show that distinct subsets of dopaminergic neurons compete to either enhance or inhibit egg-laying preference for ethanol-containing food. Moreover, activity in α′β′ neurons of the mushroom body and a subset of ellipsoid body ring neurons (R2) is required for this choice. We propose a model where competing dopaminergic systems modulate oviposition preference to adjust to changes in natural oviposition substrates.I n nature, rotting fruit is the social hub for the fruit fly Drosophila melanogaster. Flies use fermenting fruit as a food source (1) and a site for oviposition (2). The choice of a suitable oviposition substrate is an ecologically important decision with a direct impact on species fitness. However, other than having a clear preference for fermenting fruit, how females choose oviposition sites in nature is largely unknown.One of the main metabolites of fermentation is ethanol, which is present in ripe fleshy fruits (3). Although ethanol concentrations in the fruit are rather low [≤5% (vol/vol)] (4), plumes containing ethanol vapor can act as long-distance signals to attract flies to rotting fruit (3, 5). When given the choice, female flies prefer to lay their eggs on media containing low concentrations of ethanol (up to 5%) (6), which leads to enhanced fitness of the developing larva and the adult fly.D. melanogaster's resistance to ethanol toxicity may have evolved to allow inhabitation of ethanol-containing environments (7). For example, adult flies allowed to mate on ethanolcontaining media improve mating success and fecundity (8). Although rearing larvae on food containing relatively high ethanol concentrations delays development and decreases survival (9-11), larvae reared on low concentrations of ethanol develop into heavier adults (7,12). This weight increase may be a result of D. melanogaster larvae metabolizing ethanol and using it as a food source (12). Ingestion of ethanol during the larval stage has additional benefits, such as protection from natural parasites such as endoparasitoid wasps (13).Studies on the neural circuits underlying the oviposition program and choice of oviposition substrates have been initiated only recently in D. melanogaster (14, 15). Ethanol is a particularly intriguing stimulus for oviposition preference, because it has, depending on concentration, both beneficial and detrimental effects on developing larvae. In flies, the function of dopaminergic neurons has been implicated in responses to both rewarding and aversive stimuli (16-19), making it a candidate neuromodulator to signa...

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“…Two further insights have illustrated the conceptual validity of psychostimulant sensitivity in invertebrate species: first, consistent with antagonist and lesion studies in mammals (e.g., refs. [31,60]), dopamine (DA) modulates the behavioral efficacy of cocaine administration [4,35,46,58]. Moreover, fruit flies exhibit a form of behavioral sensitization [38,39] that resembles the protracted neuro-behavioral effects of psychostimulants in the mammalian brain (reviewed in refs.…”

Section: Introductionmentioning

confidence: 99%

Ethological analyses of crayfish behavior: a new invertebrate system for measuring the rewarding properties of psychostimulants

Panksepp

Huber

2004

Behavioural Brain Research

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Recent investigations in invertebrate neurobiology have opened up a new line of research into the basic behavioral, neurochemical and genomic alterations that accompany psychostimulant drug exposure. However, the extent to which such findings relate to changes in motivational and learning processes, such as those that typify drug addictions, remains unclear. The present study addressed this issue in the crayfish, Orconectes rusticus. The first set of experiments demonstrated that intramuscular injections of cocaine and amphetamine have robust and distinguishable effects on crayfish behavior. In the second part of the study, the reinforcing properties of psychostimulants were tested in a series of conditioned place preference experiments. Amphetamine and, to a lesser extent, cocaine were both found to serve as rewards when their intra-circulatory infusion was coupled to a distinct visual environment. The monoaminergic regulation of behavior has been extensively studied in decapod crustaceans and the present experiments demonstrated that (mammalian) drugs of abuse, capable of interfering with monoamine chemistry, are similarly rewarding to crayfish. Behavioral studies in crayfish can provide a complementary approach to using other invertebrate species in addiction research.

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Ectopic G-protein expression in dopamine and serotonin neurons blocks cocaine sensitization in Drosophila melanogaster

Cited by 74 publications

References 0 publications

Inositol 1,4,5-Trisphosphate Receptor and dSTIM Function inDrosophilaInsulin-Producing Neurons Regulates Systemic Intracellular Calcium Homeostasis and Flight

Inositol 1,4,5-Trisphosphate Receptor and dSTIM Function inDrosophilaInsulin-Producing Neurons Regulates Systemic Intracellular Calcium Homeostasis and Flight

Competing dopamine neurons drive oviposition choice for ethanol in Drosophila

Ethological analyses of crayfish behavior: a new invertebrate system for measuring the rewarding properties of psychostimulants

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