Ectopic Expression of Vascular Cell Adhesion Molecule-1 as a New Mechanism for Tumor Immune Evasion

Lin, Ken Y.; Lü, Dan; Hung, Chien Fu; Peng, Shiwen; Huang, Lanqing; Jie, Chunfa; Murillo, Francisco Martinez; Rowley, Jesse W.; Tsai, Ya Chea; He, Liangmei; Kim, Dae‐Jin; Jaffee, Elizabeth M.; Pardoll, Drew M.; Wu, T. C.

doi:10.1158/0008-5472.can-06-3014

Cited by 74 publications

(75 citation statements)

References 52 publications

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“…The most widely used methods for this involve examination of differential gene expression in leukemic cells by assessing the mRNA levels in the given cells. Several successful studies reported the feasibility of this approach [22][23][24][25]. On the other hand, this methodology comes from a reductionist point of view, as it neglects the dynamic nature of protein translation and further modifications that take place beyond transcription.…”

Section: Exploitation Of Protein Profiling In Leukemia Researchmentioning

confidence: 99%

The importance of protein profiling in the diagnosis and treatment of hematologic malignancies

Şanlı-Mohamed¹,

Turan²,

Ekiz³

et al. 2011

Turk J Hematol

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Proteins are important targets in cancer research because malignancy is associated with defects in cell protein machinery. Protein profiling is an emerging independent subspecialty of proteomics that is rapidly expanding and providing unprecedented insight into biological events. Quantitative assessment of protein levels in hematologic malignancies seeks a comprehensive understanding of leukemiaassociated protein patterns for use in aiding diagnosis, follow-up treatment, and the prediction of clinical outcomes. Many recently developed high-throughput proteomic methods can be applied to protein profiling. Herein the importance of protein profiling, its exploitation in leukemia research, and its clinical usefulness in the treatment and diagnosis of various cancer types, and techniques for determining changes in protein profiling are reviewed. (Turk J Hematol 2011; 28: 1-14) Key words: Hematologic malignancies, leukemia, proteomics, protein profiling Received: November 24, 2010 Accepted: January 19, 2011 ÖzetMalignitelerde proteinlerin hücresel mekanizmalarında meydana gelen bozukluklardan dolayı, proteinler kanser araştırmaları için önemli hedeflerdir. Proteomiksin alt uzmanlık dalı olarak ortaya çıkan ve bağımsız bir alan olan protein profilleme biyolojik olaylara farklı bir bakış açısı sağlamak amacıyla hızla gelişmektedir. Hematolojik malignitelerdeki protein düzeylerinin kantitatif olarak değerlendiril-mesi, teşhise yardımcı olması, tedavinin izlenmesi ve klinik sonuçların tahmininde mükemmel bir yaklaşım olması nedeni ile lösemi ile ilgili protein modellerinin kapsamlı bir şekilde incelenmesini amaçlamaktadır. Son dönemlerde geliştirilen yüksek verimli yöntemler protein profillemede kullanıla-bilir. Bu makalede, protein profillemenin önemi, lösemi araştırmalarındaki rolü, çeşitli kanser tiplerinin tanısı ve tedavisi için klinik kullanımları ve protein profilindeki değişikliklerin belirlenmesinde kullanılan teknikler değerlendirilmiştir.

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Section: Exploitation Of Protein Profiling In Leukemia Researchmentioning

confidence: 99%

The importance of protein profiling in the diagnosis and treatment of hematologic malignancies

Şanlı-Mohamed¹,

Turan²,

Ekiz³

et al. 2011

Turk J Hematol

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“…A recent report, in which the TC-1 cell line was made resistant to immune eradication in a vaccination based model, identified expression of vascular cell adhesion molecule-1 (VCAM-1) as a potential player in the immune evasion process. 127 Whether VCAM-1 could play a role in tumor eradication in cbl-b -/-mice needs to be determined. In spontaneous skin cancer formation, immune escape has not been observed so far, indicating, that immune escape from cbl-b -/-CD8 + T-cells might only occur in some cancers.…”

Section: Potential Therapeutic Use Of Cbl-b Inhibition In Cancer Immumentioning

confidence: 99%

The Ubiquitin E3 Ligase Cbl-b in T Cells Tolerance and Tumor Immunity

Loeser

Penninger²

2007

Cell Cycle

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“…Thus, to determine the role of activated PADRE-specific CD4 + T cells in the proliferation of E7-specific CD8 + T cells, we employed an E7-specific CD8 + T-cell line that expresses luciferase (E7T-LUC). 11 We have previously shown that the luminescence intensity correlates with the number of E7T-LUC cells. 11 We then performed a proliferation assay using E7T-LUC cells incubated with irradiated TC-1 cells into 24-well plates.…”

Section: Resultsmentioning

confidence: 92%

“…11 We have previously shown that the luminescence intensity correlates with the number of E7T-LUC cells. 11 We then performed a proliferation assay using E7T-LUC cells incubated with irradiated TC-1 cells into 24-well plates. PADRE-specific CD4 + T cells (PADRE-CD4) and DCs loaded with or without PADRE were added in wells as indicated in the table of Figure 6a.…”

Section: Resultsmentioning

confidence: 92%

“…22 Firefly luciferase-expressing E7-specific CD8 + T cells IL-2 enhances antigen-specific CD8 + T-cell immune responses D Kim et al (E7T-LUC) were generated using retrovirus-containing luciferase. 11 The retrovirus was produced using a pLucithy1.1 construct expressing both luciferase and thy1.1. The pLuci-thy1.1 was transfected into Phoenix-packaging cell line and the virion-containing supernatant was collected 48 h after transfection.…”

Section: Il-2 Enhances Antigen-specific Cd8 + T-cell Immune Responsesmentioning

confidence: 99%

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Role of IL-2 secreted by PADRE-specific CD4+ T cells in enhancing E7-specific CD8+ T-cell immune responses

Kim

Monie

et al. 2008

Gene Ther

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CD4 + T helper cells are known to play an integral role in the generation of CD8 + T-cell immune responses. We have previously shown that co-administration of DNA vaccines containing E6 or E7 protein of human papillomavirus 16 (HPV-16) combined with DNA encoding invariant (Ii) chain in which class II-associated Ii peptide (CLIP) region is replaced with the CD4 + T helper epitope, PADRE (Pan-DR-epitope) (Ii-PADRE DNA) enhanced HPV antigen-specific CD8 + T-cell immune responses in vaccinated mice. In the current study, we investigated the enhancement of HPV E7-specific CD8 + T-cell immune responses by PADRE-specific CD4 + T cells. We showed that intradermal administration of Ii-PADRE DNA at the same location as E7-expressing DNA is necessary to generate strong E7-specific CD8 + T-cell immune responses. We also showed that PADRE-specific CD4 + T cells generated by Ii-PADRE DNA vaccination expressed Th1 cytokine profile. Furthermore, our in vitro study demonstrated that PADRE-specific CD4 + T cells stimulated with PADRE-loaded dendritic cells secrete IL-2 that leads to the proliferation of E7-specific CD8 + T cells. Thus, our data suggest that activated PADRE-specific CD4 + T helper cells may be required at the vicinity of E7-specific CD8 + T cells where they secrete IL-2, which enhances the E7-specific CD8 + T-cell immune responses generated by DNA vaccination.

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Ectopic Expression of Vascular Cell Adhesion Molecule-1 as a New Mechanism for Tumor Immune Evasion

Cited by 74 publications

References 52 publications

The importance of protein profiling in the diagnosis and treatment of hematologic malignancies

The importance of protein profiling in the diagnosis and treatment of hematologic malignancies

The Ubiquitin E3 Ligase Cbl-b in T Cells Tolerance and Tumor Immunity

Role of IL-2 secreted by PADRE-specific CD4+ T cells in enhancing E7-specific CD8+ T-cell immune responses

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