Ectopic Expression of Two FOREVER YOUNG FLOWER Orthologues from Cattleya Orchid Suppresses Ethylene Signaling and DELLA Results in Delayed Flower Senescence/Abscission and Reduced Flower Organ Elongation in Arabidopsis

Chen, Wei-Han; Lee, Yung-I; Yang, Chang‐Hsien

doi:10.1007/s11105-018-1114-y

Cited by 8 publications

(4 citation statements)

References 51 publications

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“…However, we hypothesized that how LcAGL18 regulates litchi fruitlet abscission might be different with FYF homologs since LcAGL18 and FYF orthologs showed different expression patterns before abscission. For FYF orthologs, such as AtFYF, CaFYF1/2, and PaFYF1/2, which were highly expressed at the young floral buds and mature flowers but were significantly decreased before abscission [26,27,48]. By contrast, the expression of LcAGL18 was greatly enhanced during the litchi fruitlet abscission (Figure 2C).…”

Section: Discussionmentioning

confidence: 98%

“…In Arabidopsis, overexpression of the MADS-box TFs FYF1/2 resulted in a significant delay of floral organ abscission [46]. Similarly, ectopic expression of FYF orthologs in Arabidopsis, including OnFYF, CaFYF1/2, and PaFYF1/2, also delayed the floral organ abscission [25][26][27]47]. FYF orthologs were found to function similarly as repressors in regulating floral organ abscission by repressing ethylene response genes such as ethylene response DNA-binding factors (EDFs) and abscission-associated genes such as BLAD ON PETIOLE (BOP1/2) and IDA [48].…”

Section: Discussionmentioning

confidence: 99%

“…AGL15 overexpression leads to a reduction in HAE expression, resulting in a prolonged abscission [24]. Overexpression of FOREVER YOUNG FLOWER (a MADS-box gene) orthologs from Oncidium orchids (OnFYF), Cattleya orchids (CaFYF1/2), and Phalaenopsis orchids (PaFYF1/2) in transgenic Arabidopsis delays floral organ abscission [25][26][27]. These observations suggest the significant roles of MADSbox TFs in organ abscission in model plants and orchid species.…”

Section: Introductionmentioning

confidence: 98%

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Ectopic Expression of AGAMOUS-like 18 from Litchi (Litchi chinensis Sonn.) Delayed the Floral Organ Abscission in Arabidopsis

Wang

Zhang

et al. 2023

Horticulturae

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The regulation of abscission has a significant impact on fruit yield and quality. Thus, understanding the mechanisms underlying abscission, particularly identifying key genes, is critical for improving fruit crop breeding and cultivation practices. Here, to explore the key genes involved in litchi fruitlet abscission, the two closest homologs of AGAMOUS-like 15/18 (LcAGL15 and LcAGL18) were identified. During the litchi fruitlet abscission process, LcAGL15 expression was reduced, whereas LcAGL18 expression was increased at the abscission zone. The abscission of floral organs was unaffected by ectopic expression of LcAGL15 in Arabidopsis. Moreover, high expression of LcAGL18 significantly delayed the abscission process of floral organs, particularly the sepals. Overexpression of LcAGL18 in Arabidopsis consistently repressed the expression of abscission-related genes, including HAESA (HAE) and HAESA-LIKE2 (HSL2), and cell wall remodeling genes at the abscission zone. Furthermore, LcAGL18 was localized in the nucleus and acted as a transcriptional inhibitor. Collectively, these results suggest that AGL18 homologs have conserved functions in Arabidopsis and litchi, and that LcAGL18 might function as a key regulator in litchi fruitlet abscission.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Ectopic Expression of AGAMOUS-like 18 from Litchi (Litchi chinensis Sonn.) Delayed the Floral Organ Abscission in Arabidopsis

Wang

Zhang

et al. 2023

Horticulturae

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“…Total RNA was extracted 44 , 45 and used for quantitative real-time PCR which was conducted using a C1000 thermal cycler/Bio-Rad CFX96 Touch real-time PCR detection system (Bio-Rad, Hercules, California, USA) and Optical System Software version 3.0a (Bio-Rad Laboratories). For transcript measurements, the ChamQ Universal SYBR qPCR Master Mix (Vazyme Biotech Co., Ltd) was used.…”

Section: Methodsmentioning

confidence: 99%

OAF is a DAF-like gene that controls ovule development in plants

Lin

Hsu

et al. 2023

Commun Biol

Self Cite

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We previously found that the RING-type E3 ligase DEFECTIVE IN ANTHER DEHISCENCE1- (DAD1-) Activating Factor (DAF) controls anther dehiscence by activating the jasmonate biosynthetic pathway in Arabidopsis. Here, we show that in Arabidopsis, the DAF ancestor was duplicated into three genes (DAF, Ovule Activating Factor (OAF), DAFL2), which evolved divergent partial functions from their ancestor through subfunctionalization. In this case, DAF-DAD1-JA signaling regulates anther dehiscence, whereas OAF controls ovule development by negatively regulating cinnamyl alcohol dehydrogenase 9 (CAD9) activity and being negatively regulated by miR847 itself in Arabidopsis. Downregulation of OAF or upregulation of CAD9 and miR847 caused similar abortion of ovule formation due to precocious ovule lignification in transgenic Arabidopsis. Interestingly, only one DAF-like gene, PaOAF, exists in the monocot orchids, which has likely evolved through nonfunctionalization and maintains a conserved function as Arabidopsis OAF in regulating ovule development since defective ovules were observed in the virus-induced gene silencing (VIGS) PaOAF Phalaenopsis orchids. The absence of the DAF ortholog and its function in orchids is likely due to the evolution of stamens to a unique pollinium structure that lacks the feature of anther dehiscence. These findings expand the current knowledge underlying the multifunctional evolution and diverse functionalization of duplicate gene pairs within/among plants.

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Regulatory Mechanisms Underlying Activation of Organ Abscission

Jiang

Gao

2021

Annual Plant Reviews Online

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Abscission is a tightly regulated developmental process that initiates in response to endogenous and environmental cues. In an agricultural context, regulation of abscission has substantial effects on crop productivity and quality. Crop domestication often selected against abscission of the fruit, allowing easier harvesting. A better understanding of the mechanisms underlying abscission will, therefore, inform efforts to improve crop breeding and harvesting practices. In this article, we summarize our current knowledge of the various regulatory mechanisms involved in abscission. We first review the regulation of abscission zone cells as they acquire competence in response to abscission signals. We then summarize the regulation of abscission initiation, exploring the phytohormone signals, developmental factors, and environmental cues regulating the initiation of abscission.

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Ectopic Expression of Two FOREVER YOUNG FLOWER Orthologues from Cattleya Orchid Suppresses Ethylene Signaling and DELLA Results in Delayed Flower Senescence/Abscission and Reduced Flower Organ Elongation in Arabidopsis

Cited by 8 publications

References 51 publications

Ectopic Expression of AGAMOUS-like 18 from Litchi (Litchi chinensis Sonn.) Delayed the Floral Organ Abscission in Arabidopsis

Ectopic Expression of AGAMOUS-like 18 from Litchi (Litchi chinensis Sonn.) Delayed the Floral Organ Abscission in Arabidopsis

OAF is a DAF-like gene that controls ovule development in plants

Regulatory Mechanisms Underlying Activation of Organ Abscission

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