Ectopic expression of the maize chromosomal HMGB1 protein causes defects in root development of tobacco seedlings

Lichota, Jacek; Ritt, Christoph; Grasser, Klaus D.

doi:10.1016/j.bbrc.2004.03.193

Cited by 18 publications

(13 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…31 Therefore, we have examined the root growth of plants grown in solid MS medium. The length of the primary root of (c) rtPCR analysis of RNA isolated from Col-0 and hmgb1 seedlings using HMGB1-specific primers (P1 + P2), and for comparison, the PCR was performed using DNA isolated from Col-0.…”

Section: Altered Levels Of Hmgb1 Affect Vegetative Developmentmentioning

confidence: 99%

“…30 Ectopic expression of maize HMGB1 in tobacco seedlings caused reduced length of the primary root, whereas HMGB4 did not affect root development. 31 In Arabidopsis, overexpression of HMGB2 reduced seed germination under salt and drought stress. 8 The Arabidopsis genome encodes seven HMGB proteins that differ in structure, expression pattern, and DNA-binding properties, 8,9,20,[32][33][34] suggesting (partially) specific functions of the different family members.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Expression Level of the Chromatin-Associated HMGB1 Protein Influences Growth, Stress Tolerance, and Transcriptome in Arabidopsis

Lildballe

Pedersen

Kalamajka

et al. 2008

Journal of Molecular Biology

View full text Add to dashboard Cite

Section: Altered Levels Of Hmgb1 Affect Vegetative Developmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Expression Level of the Chromatin-Associated HMGB1 Protein Influences Growth, Stress Tolerance, and Transcriptome in Arabidopsis

Lildballe

Pedersen

Kalamajka

et al. 2008

Journal of Molecular Biology

View full text Add to dashboard Cite

“…Notably, the HMGB1 gene was expressed at relatively high levels in pericycle and nonpericycle central cylinder cells. It has been demonstrated that ectopic expression of this maize gene in tobacco (Nicotiana tabacum) seedlings specifically affects root development, leading to reduced primary root elongation and cortical cell size in primary roots, whereas aboveground development of these plants was not altered (Lichota et al, 2004).…”

Section: Pericycle-specific Est Sequencing Reveals Putative Novel Genmentioning

confidence: 99%

Transcriptomic and Proteomic Analyses of Pericycle Cells of the Maize Primary Root

et al. 2007

View full text Add to dashboard Cite

Each plant cell type expresses a unique transcriptome and proteome at different stages of differentiation dependent on its developmental fate. This study compared gene expression and protein accumulation in cell-cycle-competent primary root pericycle cells of maize (Zea mays) prior to their first division and lateral root initiation. These are the only root cells that maintain the competence to divide after they leave the meristematic zone. Pericycle cells of the inbred line B73 were isolated via laser capture microdissection. Microarray experiments identified 32 genes preferentially expressed in pericycle versus all other root cells that have left the apical meristem; selective subtractive hybridization identified seven genes preferentially expressed in pericycle versus central cylinder cells of the same root region. Transcription and protein synthesis represented the most abundant functional categories among these pericycle-specific genes. Moreover, 701 expressed sequence tags (ESTs) were generated from pericycle and central cylinder cells. Among those, transcripts related to protein synthesis and cell fate were significantly enriched in pericycle versus nonpericycle cells. In addition, 77 EST clusters not previously identified in maize ESTs or genomic databases were identified. Finally, among the most abundant soluble pericycle proteins separated via twodimensional electrophoresis, 20 proteins were identified via electrospray ionization-tandem mass spectrometry, thus defining a reference dataset of the maize pericycle proteome. Among those, two proteins were preferentially expressed in the pericycle. In summary, these pericycle-specific gene expression experiments define the distinct molecular events during the specification of cell-cycle-competent pericycle cells prior to their first division and demonstrate that pericycle specification and lateral root initiation might be controlled by a different set of genes.

show abstract

“…Currently, there are only few reports addressing the role of plant HMGB-type proteins in development or in response to changing environmental conditions. Ectopic expression of maize HMGB1 in tobacco seedlings results in reduced length of the primary root, whereas the shoot of the seedlings had wild-type appearance [117]. This "short-root" phenotype of the transgenic lines correlated with a decreased size of the cells in the cell division zone of the root.…”

mentioning

confidence: 98%

The HMG-box: a versatile protein domain occurring in a wide variety of DNA-binding proteins

Štros

Launholt

Grasser

2007

Cell. Mol. Life Sci.

252

231

View full text Add to dashboard Cite

The HMG-box domain of approximately 75 amino acid residues was originally identified as the domain that mediates the DNA-binding of chromatin-associated high-mobility group (HMG) proteins of the HMGB type. In the last few years, HMG-box domains have been found in various DNA-binding proteins including transcription factors and subunits of chromatin-remodeling complexes. HMG-box domains mediate either non-sequence-specific (e.g., HMGB-type proteins) or sequence-specific (e.g., transcription factors) DNA binding. Both types of HMG-box domains bind non-B-type DNA structures (bent, kinked and unwound) with high affinity. In addition, HMG-box domains are involved in a variety of protein-protein interactions. Here, we have examined the human and plant genomes for genes encoding HMG-box domains. Compared to plants, human cells contain a larger variety of HMG-box proteins. Whereas in humans transcription factors are the most divergent group of HMG-box proteins, in plants the chromosomal HMGB-type proteins are most variable.

show abstract

Ectopic expression of the maize chromosomal HMGB1 protein causes defects in root development of tobacco seedlings

Cited by 18 publications

References 32 publications

The Expression Level of the Chromatin-Associated HMGB1 Protein Influences Growth, Stress Tolerance, and Transcriptome in Arabidopsis

The Expression Level of the Chromatin-Associated HMGB1 Protein Influences Growth, Stress Tolerance, and Transcriptome in Arabidopsis

Transcriptomic and Proteomic Analyses of Pericycle Cells of the Maize Primary Root

The HMG-box: a versatile protein domain occurring in a wide variety of DNA-binding proteins

Contact Info

Product

Resources

About