Coding sequences (CDS) are commonly used for transient gene expression, in yeast two-hybrid screening, to verify protein interaction verification interactions and in prokaryotic gene expression studies. CDS are most common method to obtain CDS is to utilize commonly obtained using complementary DNA (cDNA) as a template, which derived from message RNA (mRNA) extracted from plant tissues and generated by reverse transcription. However, some CDSs are difficult to acquire with through regular way due to the process as they are expressed at extremely low gene expressions levels or have specific spatial and/or temporal expression patterns in vivo, so it is necessary to develop. These challenges require the development of alternative CDS cloning technologies. In this study, we found that the genomic intron-containing gene coding sequences (gDNA) from Arabidopsis thaliana, Oryza sativa, Brassica napus, and Glycine max can be correctly transcribed and spliced into mRNA in Nicotiana benthamiana (N. benthamiana). In contrast, gDNAs from Triticum aestivum and Sorghum bicolor did not function correctly. In transient expression experiments, the target DNA sequence can is driven by a constitutive promoter. Theoretically, a sufficient amount of mRNA can be extracted from the N. benthamiana leaves, which is making it conducive to the cloning of CDS of target genes. Our data demonstrate that N. benthamiana can be employed as an effective host for the cloning CDS of plant genes.