Ectopic expression of sugarcane SHINE changes cell wall and improves biomass in rice

Biomass Accumulation and Cell Wall Structure of Rice Plants Overexpressing a Dirigent-Jacalin of Sugarcane (ShDJ) Under Varying Conditions of Water Availability

“…A reduction in expression was observed by Ambavaram et al (2011), and AtSHN -overexpression in rice directly repressed OsMYB58/63 and OsNST1/2 in leaves and stems, resulting in a large reduction in lignin content. Recently, Martins et al (2018) demonstrated that the overexpression of sugarcane ShSHN1 in rice repressed OsMYB58/63 and OsNST1/2 in leaves and tillers followed by a decrease in lignin content in the transgenic lines and improvement of saccharification efficiency. Interestingly, ShSHN1 rice transgenic lines showed an increase in biomass production when compared with WT plants.…”

“…Analyses of transgene expression level ( n = 3 ± standard error) were conducted using rice eukaryotic elongation factor-1α gene (Accession No. AK061464) as endogenous control to normalize the cDNA variance between samples (Martins et al, 2018).…”

“…The hpt II and sucrose phosphate synthase ( SPS ) primers and TaqMan probes were synthesized by Applied Biosystems (Foster City, CA, United States) and used in all analyses (Supplementary Table 1) (Ding et al, 2004). RT-qPCR and thermal profile reactions were performed as described by Martins et al (2018) and the transgene copy number was determined as described by Mason et al (2002) method.…”

“…Cell wall polysaccharides were evaluated as described by Chen et al (2002) and the soluble and insoluble lignin contents (Klason’s Methods) were determined following the Tappi protocol UM-250 (TAPPI, 1985). The saccharification ratio was measured as described by methodology of Brown and Torget (1996), with modifications as described by Martins et al (2018).…”

Analysis of biomass traits in sugarcane (Saccharum spp. hybrids)

“…More than 50 DE transcripts corresponded to transcription factor (TF) genes from 21 gene families involved in secondary cell wall synthesis including AP2/EREB, ARF, AUX/IAA, B3, BBX, bHLH, bZIP, C2H2, C3H, Dof, FRS, GRAS, HB, LIM, MADS-box, MYB, MYB-related, PHD, SAP, TIFY and WRKY (Table4.4.4). Members of these families have been shown to regulate lignin biosynthesis and consequently, influence lignin content and composition[273][274][275]. MYB TFs are considered particularly important because they activate other TFs including lignin-specific MYBs, which control lignin biosynthesis by directly binding to the promoters of genes, such as, CAD, CCR and 4CL…”