Ectopic expression of SOD and APX genes in Arabidopsis alters metabolic pools and genes related to secondary cell wall cellulose biosynthesis and improve salt tolerance

Shafi, Amrina; Gill, Tejpal; Zahoor, Insha; Ahuja, Paramvir Singh; Sreenivasulu, Yelam; Kumar, Sanjay; Singh, Anil Kumar

doi:10.1007/s11033-019-04648-3

Cited by 34 publications

(20 citation statements)

References 58 publications

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“…Additionally, A. thaliana transgenics overexpressing cytosolic CuZn-superoxide dismutase (PaSOD) from Potentilla atrosanguinea, cytosolic ascorbate peroxidase (PaSOD) from Rheum australe and dual transgenics overexpressing both the genes were developed and analyzed under salt stress. In comparison to wild-type (WT) or single transgenics, the performance of dual transgenics under salt stress was better with higher biomass accumulation and cellulose content at 100 mM salt stress [42]. However, in numerous studies with transgenic plants, no attention has been paid to structural conversions manifesting at the cellular level.…”

Section: Discussionmentioning

confidence: 99%

Salt Stress-Induced Structural Changes Are Mitigated in Transgenic Tomato Plants Over-Expressing Superoxide Dismutase

Bogoutdinova

Лазарева

Chaban

et al. 2020

Biology

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Various abiotic stresses cause the appearance of reactive oxygen species (ROS) in plant cells, which seriously damage the cellular structures. The engineering of transgenic plants with higher production of ROS-scavenging enzyme in plant cells could protect the integrity of such a fine intracellular structure as the cytoskeleton and each cellular compartment. We analyzed the morphological changes in root tip cells caused by the application of iso-osmotic NaCl and Na2SO4 solutions to tomato plants harboring an introduced superoxide dismutase gene. To study the roots of tomato plants cultivar Belyi Naliv (WT) and FeSOD-transgenic line, we examined the distribution of ROS and enzyme-linked immunosorbent detection of α-tubulin. In addition, longitudinal sections of the root apexes were compared. Transmission electronic microscopy of atypical cytoskeleton structures was also performed. The differences in the microtubules cortical network between WT and transgenic plants without salt stress were detected. The differences were found in the cortical network of microtubules between WT and transgenic plants in the absence of salt stress. While an ordered microtubule network was revealed in the root cells of WT tomato, no such degree of ordering was detected in transgenic line cells. The signs of microtubule disorganization in root cells of WT plants were manifested under the NaCl treatment. On the contrary, the cytoskeleton structural organization in the transgenic line cells was more ordered. Similar changes, including the cortical microtubules disorganization, possibly associated with the formation of atypical tubulin polymers as a response to salt stress caused by Na2SO4 treatment, were also observed. Changes in cell size, due to both vacuolization and impaired cell expansion in columella zone and cap initials, were responsible for the root tip tissue modification.

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Section: Discussionmentioning

confidence: 99%

Salt Stress-Induced Structural Changes Are Mitigated in Transgenic Tomato Plants Over-Expressing Superoxide Dismutase

Bogoutdinova

Лазарева

Chaban

et al. 2020

Biology

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“…Although its role in regulating secondary wall formation and its regulation mode of activating gene expression by binding elements such as SMRE, MYBCORE and AC‐box in the promoter region have been elucidated (Nakano et al ., ; Zhong and Ye, ), its function in other metabolic (such as disease susceptibility) and signal transduction pathways in plants is still being explored (Ramírez et al ., ). In recent years, MYB46 was found to be activated under stress (Geng et al ., ; Shafi et al ., ), and it was put forward that BplMYB46 might have the function of regulating the expression of genes involved in abiotic stress responses of Betula platyphylla (Guo et al ., ). However, the genes and metabolic pathways that could be specifically regulated by MYB46 under stress remain to be further explored and determined.…”

Section: Introductionmentioning

confidence: 99%

MdMYB46 could enhance salt and osmotic stress tolerance in apple by directly activating stress‐responsive signals

Chen

Song

Guo

et al. 2019

Plant Biotechnology Journal

159

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Summary To expand the cultivation area of apple (Malus×domestica Borkh.) and select resistant varieties by genetic engineering, it is necessary to clarify the mechanism of salt and osmotic stress tolerance in apple. The MdMYB46 transcription factor was identified, and the stress treatment test of MdMYB46‐overexpressing and MdMYB46‐RNAi apple lines indicated that MdMYB46 could enhance the salt and osmotic stress tolerance in apple. In transgenic Arabidopsis and apple, MdMYB46 promoted the biosynthesis of secondary cell wall and deposition of lignin by directly binding to the promoter of lignin biosynthesis‐related genes. To explore whether MdMYB46 could coordinate stress signal transduction pathways to cooperate with the formation of secondary walls to enhance the stress tolerance of plants, MdABRE1A, MdDREB2A and dehydration‐responsive genes MdRD22 and MdRD29A were screened out for their positive correlation with osmotic stress, salt stress and the transcriptional level of MdMYB46. The further verification test demonstrated that MdMYB46 could activate their transcription by directly binding to the promoters of these genes. The above results indicate that MdMYB46 could enhance the salt and osmotic stress tolerance in apple not only by activating secondary cell wall biosynthesis pathways, but also by directly activating stress‐responsive signals.

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“…Previous studies have speculated that lignin monomer-coupled polymeric proteins mainly include dirigent (DIR), peroxidase (POD), laccase (LAC), superoxide dismutase (SOD) and nicotinamide adenine dinucleotide phosphate (NADPH) oxidases [8]. Notably, reactive oxygen species (ROS) play an important role in the lignification of plant cells [8,28,29,30,31]. Plant NADPH oxidase, also known as respiratory burst oxidase homolog (RBOH) [28], is a homolog of the mammalian macrophage NADPH oxidase catalytic subunit gp91 phox [8,32].…”

Section: Introductionmentioning

confidence: 99%

In Silico Genome-Wide Analysis of Respiratory Burst Oxidase Homolog (RBOH) Family Genes in Five Fruit-Producing Trees, and Potential Functional Analysis on Lignification of Stone Cells in Chinese White Pear

Cheng

Manzoor

et al. 2019

Cells

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The accumulation of lignin in fruit has a significant negative impact on the quality of fruit-producing trees, and in particular the lignin formation stimulates the development of stone cells in pear fruit. Reactive oxygen species (ROS) are essential for lignin polymerization. However, knowledge of the RBOH family, a key enzyme in ROS metabolism, remains unknown in most fruit trees. In this study, a total of 40 RBOHs were identified from five fruit-producing trees (Pyrus bretschneideri, Prunus persica, Citrus sinensis, Vitis vinifera, and Prunus mume), and 10 of these sequences came from Pyrus bretschneideri. Multiple sequence alignments revealed that all 10 PbRBOHs contained the NADPH_Ox domain and the six alpha-helical transmembrane domains (TM-I to TM-VI). Chromosome localization and interspecies phylogenetic tree analysis showed that 10 PbRBOHs irregularly distributed on 8 chromosomes and 3 PbRBOHs (PbRBOHA, PbRBOHB, and PbRBOHD) are closely related to known lignification-related RBOHs. Furthermore, hormone response pattern analysis showed that the transcription of PbRBOHs is regulated by SA, ABA and MeJA. Reverse transcription-quantitative real-time polymerase chain reaction (qRT-PCR) and transcriptome sequencing analysis showed that PbRBOHA, PbRBOHB, and PbRBOHD accumulated high transcript abundance in pear fruit, and the transcriptional trends of PbRBOHA and PbRBOHD was consistent with the change of stone cell content during fruit development. In addition, subcellular localization revealed that PbRBOHA and PbRBOHD are distributed on the plasma membrane. Combining the changes of apoplastic superoxide (O2.−) content and spatio-temporal expression analysis, these results indicate that PbRBOHA and PbRBOHD, which are candidate genes, may play an important role in ROS metabolism during the lignification of pear stone cells. This study not only provided insight into the molecular characteristics of the RBOH family in fruit-producing trees, but also lays the foundation for studying the role of ROS in plant lignification.

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Ectopic expression of SOD and APX genes in Arabidopsis alters metabolic pools and genes related to secondary cell wall cellulose biosynthesis and improve salt tolerance

Cited by 34 publications

References 58 publications

Salt Stress-Induced Structural Changes Are Mitigated in Transgenic Tomato Plants Over-Expressing Superoxide Dismutase

Salt Stress-Induced Structural Changes Are Mitigated in Transgenic Tomato Plants Over-Expressing Superoxide Dismutase

MdMYB46 could enhance salt and osmotic stress tolerance in apple by directly activating stress‐responsive signals

In Silico Genome-Wide Analysis of Respiratory Burst Oxidase Homolog (RBOH) Family Genes in Five Fruit-Producing Trees, and Potential Functional Analysis on Lignification of Stone Cells in Chinese White Pear

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