“…Primary antisera used were against GnRH [polyclonal SW-1, 1:3000, (Wray et al, 1988)], peripherin (1:2000, Chemicon, Temecula, CA), p75NGFR (1:5000, Chemicon), nestin (1:3000, Kramer et al, 2000b), S100 (1:3000–4000, Dako, Glostrup, DN), GAD67 (1:8000, gift from L. J. Kopin; Oertel et al, 1981; Lee et al, 2008), PSA-NCAM (1:4000, Millipore, Billerica, MA), GFAP (Glial Fibrillary Acidic Protein, 1:18, Incstar, Stillwater, MN), Sox-10 (1:400, Forni et al, 2011), neurophysin (RN2, 1:12000, Wray et al, 1988), S100 (1:1000, Dako, Glostrup, DN), and Caspase-3 (1:200, Millipore, Billerica, MA). Alexa Fluor-546 phalloidin, (1:40, Molecular Probe, Eugene, OP) was used to visualize actin filaments.…”