Ectopic expression of LEAFY COTYLEDON1-LIKE gene and localized auxin accumulation mark embryogenic competence in epiphyllous plants of Helianthus annuus × H. tuberosus

Chiappetta, Adriana; Fambrini, Marco; Petrarulo, M.; Rapparini, Francesca; Michelotti, V.; Bruno, Leonardo; Greco, Maria; Baraldi, Rita; Salvini, Mariangela; Pugliesi, Claudio; Bitonti, Maria Beatrice

doi:10.1093/aob/mcn266

Cited by 35 publications

(26 citation statements)

References 77 publications

(101 reference statements)

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“…Expression of LEC2 (Ledwoń and Gaj, 2009) together with LEC1 and FUS3 (Ledwoń and Gaj, 2011) was observed in callus tissue developing on auxin-rich medium. As in sunflower (Fambrini et al, 2006;Chiappetta et al, 2009), LEC1 expression was not observed in cultures induced to regenerate shootlike structures, but the gene was highly active in embryogenic cells.…”

Section: Discussionmentioning

confidence: 86%

Immature Zygotic Embryo Cultures of Arabidopsis. A Model System for Molecular Studies on Morphogenic Pathways Induced In Vitro

Kraut¹,

Wójcikowska²,

Ledwoń³

et al. 2011

Acta Biologica Cracoviensia Series Botanica

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To understand the molecular mechanism controlling in vitro plant morphogenesis, a culture system enabling induction of alternative morphogenic pathways (somatic embryogenesis, SE; shoot organogenesis, ORG) in a well defined population of somatic cells is needed. Arabidopsis is the most useful model plant for genomic studies, but a system in which SE or ORG can be induced alternatively in the same type of explant has not been proposed. Immature zygotic embryos (IZEs) of Arabidopsis provide the only explants with embryogenic potential, and have been recommended for studying mechanisms of SE induced in vitro. This study was aimed at defining culture conditions promoting induction of alternative morphogenic pathways: shoot ORG in IZE explants. The established protocol involves pretreatment of IZE explants with liquid auxin-rich callus induction (CIM) medium, followed by subculture on solid cytokinin-rich shoot induction medium (SIM). The method enables efficient shoot induction in Columbia (Col-0) and Wassilewskija (Ws), genotypes commonly used in molecular studies. During 3 weeks of culture up to 90% of Col-0 and 70% of Ws explants regenerated shoots via an indirect morphogenic pathway. We analyzed the qRT-PCR expression patterns of the LEC (LEC1, LEC2 and FUS3) genes, the key regulators of Arabidopsis embryogenesis, in the IZE explants induced to promote shoot ORG. The sharp decline of LEC expression on SIM medium confirmed that culture of Arabidopsis IZE explants enables experimental manipulation of the morphogenic response of somatic cells. A scheme illustrating various in vitro morphogenic responses of IZEs in relation to hormonal treatment is presented.K Ke ey y w wo or rd ds s: : Arabidopsis, immature zygotic embryos, in vitro morphogenesis, shoot regeneration.

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Section: Discussionmentioning

confidence: 86%

Immature Zygotic Embryo Cultures of Arabidopsis. A Model System for Molecular Studies on Morphogenic Pathways Induced In Vitro

Kraut¹,

Wójcikowska²,

Ledwoń³

et al. 2011

Acta Biologica Cracoviensia Series Botanica

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“…DIG-labeled RNA sense and anti-sense probes were obtained as described in Bruno et al (2009a). Immunological detection was as reported by Chiappetta et al (2009). Transcript accumulation was visualized as a violet/brown staining.…”

Section: Methodsmentioning

confidence: 99%

“…Fifteen µl were electrophoresed in a 1% agarose gel; each signal was determined using quantitative optical densitometry (OD ± SD) based on the pixel intensity/ area of each PCR product as described in Chiappetta et al (2009). Three replicate analyses were performed and density differences were evaluated using Tukey's test.…”

Section: Methodsmentioning

confidence: 99%

PoCHL P expression pattern in Posidonia oceanica is related to critical light conditions

Bruno¹,

Bruno²,

Chiappetta³

et al. 2010

Mar. Ecol. Prog. Ser.

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In past decades, meadows of Posidonia oceanica (L) Delile have declined extensively in the Mediterranean basin mainly due to disturbances associated with reduced light availability. To investigate the molecular mechanisms of photoacclimation in this seagrass, we selected the CHL P gene as a marker of plant response to light availability because it is involved in the chlorophylltocopherols biosynthetic pathways. After characterizing the PoCHL P gene, its expression pattern was analyzed in leaves of plants growing in 2 distinct meadows that were previously categorized as disturbed and preserved. Plants were sampled during different seasons and light availability was monitored in the selected meadows over a 1 yr period. PoCHL P expression was also analyzed in leaves of plants that were exposed to controlled light conditions in aquaria. We found that PoCHL P was expressed differentially with respect to leaf developmental stage and that its expression pattern changed rapidly under light conditions far below the compensation value established for P. oceanica. Hence, the recognition of this gene as a reasonable marker of critical light conditions provides an important step towards the functional characterization of stress-related genes in seagrasses. KEY WORDS: PoCHL P expression · Posidonia oceanica · Light availabilityResale or republication not permitted without written consent of the publisher

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“…The altered cell division in EMS mutant as well as the level of TOZ and TTN6 gene transcripts at different stage of seed development suggested that TOZ and TTN6 genes could also play a direct role in Phaseolus in regulating cell division. Interestingly, the transcript levels of several genes required for controlling embryo and seed development, such as PEI (Li and Thomas 1998), and for coordinating the morphogenesis phases, such as L1L (Chiappetta et al 2009), were altered in EMS mutant relative to the wild-type (Fig. 5).…”

Section: Plant Cell Tiss Organ Cultmentioning

confidence: 99%

In silico identification and characterization of putative differentially expressed genes involved in common bean (Phaseolus vulgaris L.) seed development

Abid

Muhovski

Jacquemin

et al. 2011

Plant Cell Tiss Organ Cult

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Two genotypes of common bean (Phaseolus vulgaris L.) were studied to determine the structural cause of seed abortion in this species. In the non-abortive control (wild-type, cultivar BAT93), the histological analysis revealed a classical pattern of seed development and showed coordinated differentiation of the embryo proper, suspensor, endosperm tissue and seed coat. In contrast, the ethyl methanesulfonate (EMS) mutant (cultivar BAT93) showed disruption in the normal seed development leading to embryo abortion. Aborted embryos from these degenerate seeds showed abnormalities in suspensor and cotyledons at the globular, heart, torpedo and cotyledon stages. Exploring the feasibility of incorporating the available online bioinformatics databases, we identified 22 genes revealing high homology with genes involved in Arabidopsis thaliana embryo development and expressed in common bean immature seeds. The expression patterns of these genes were confirmed by RT-PCR. All genes were highly expressed in seed tissues. To study the expression profiles of isolated genes during Phaseolus embryogenesis, six selected genes were examined by quantitative RT-PCR analysis on the developing embryos of wild-type and EMS mutant plants. All selected genes were expressed differentially at different stages of embryo development. These results could help to improve understanding of the mechanism of common bean embryogenesis.

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Ectopic expression of LEAFY COTYLEDON1-LIKE gene and localized auxin accumulation mark embryogenic competence in epiphyllous plants of Helianthus annuus × H. tuberosus

Cited by 35 publications

References 77 publications

Immature Zygotic Embryo Cultures of Arabidopsis. A Model System for Molecular Studies on Morphogenic Pathways Induced In Vitro

Immature Zygotic Embryo Cultures of Arabidopsis. A Model System for Molecular Studies on Morphogenic Pathways Induced In Vitro

PoCHL P expression pattern in Posidonia oceanica is related to critical light conditions

In silico identification and characterization of putative differentially expressed genes involved in common bean (Phaseolus vulgaris L.) seed development

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