Ectopic expression of GmHP08 enhances resistance of transgenic Arabidopsis toward drought stress

Chuong, Nguyen Nguyen; Hoang, Xuan Lan Thi; Nghia, Duong Hoang Trong; Nguyen, Nguyen Cao; Thao, Dau Thi Thanh; Tran, Tram Bao; Ngoc, Tran Thi My; Thư, Nguyễn Bình Anh; Nguyen, Quang; Thảo, Nguyễn Phương

doi:10.1007/s00299-021-02677-6

Cited by 8 publications

(7 citation statements)

References 119 publications

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“…Regarding GmHP08, it was found that expression of this gene was significantly induced in the soybean shoot tissue after 10 hours of dehydration treatment (Le et al, 2011) or under 15-day-drought stress conditions (Thu et al, 2015). Subsequent in planta study indicated that transgenic Arabidopsis carrying GmHP08 acquired better drought tolerance (Chuong et al, 2021), confirming the critical role of this protein in response to drought.…”

Section: Introductionmentioning

confidence: 92%

Expression alteration analyses in the transgenic Arabidopsis carrying soybean Histidine-containing phosphotransmitter gene under salinity stress condition

Hung

Xuan

Quang

et al. 2022

Vietnam J. Biotechnol.

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Productivity of many crops is highly vulnerable to extreme external conditions. Environmental stress factors such as drought and salinity have become more and more serious due to climate change and appear in many areas worldwide with higher frequency. As both drought and salinity belong to osmotic stress, they have similar negative effects on plant growth, development, and productivity as well as trigger similar stress responses by plants. In a previous study analyzing the expression profile in two soybean (Glycine max) cultivars with contrasting drought-tolerant phenotypes, a member of two-component system (TCS) in soybean, GmHP08, was proposed to associate with the plant tolerance capacity to drought. Subsequent in planta study confirmed its action as a positive regulator under drought conditions, as the transgenic Arabidopsis plants ectopically expressing GmHP08 acquired better drought tolerance. Following this, the presented research further explored the possible function of GmHP08 in mediating plant response to salinity. The obtained data from RT-qPCR analyses suggested that GmHP08 might positively enhance the salt tolerance of the Arabidopsis transgenic plants by altering the transcriptional abundance of several stress-related genes, including RD29A, RD29B, ABI5, SAG13, and CSD1. Activities of these genes are known to be associated with osmoprotection, senescence process, and antioxidation, which contribute to salt-tolerance ability of the transgenic plants. These results provided the first line of molecular evidence regarding GmHP08 function in plant response to salinity conditions. Therefore, extensive studies should be conducted in future studies to elaborate on the mechanisms by which this TCS member could improve various types of osmotic stress tolerance in plants.

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Section: Introductionmentioning

confidence: 92%

Expression alteration analyses in the transgenic Arabidopsis carrying soybean Histidine-containing phosphotransmitter gene under salinity stress condition

Hung

Xuan

Quang

et al. 2022

Vietnam J. Biotechnol.

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“…In rice plants, upregulation of OsMYB48–1 boosted the expression of genes produce ABA such as OsNCED4 and OsNCED5 [ 41 ]. GmHP08 improves drought tolerance in soybeans via ABA-dependent pathways [ 42 ]. Drought resistance in Arabidopsis thaliana is improved by overexpression of the AtSAUR32 gene, which accumulates ABA and IAA hormones [ 43 ].…”

Section: Drought-responsive Genes Are Regulated By Plant Hormonesmentioning

confidence: 99%

Molecular Aspects of MicroRNAs and Phytohormonal Signaling in Response to Drought Stress: A Review

Ahmad

Wang

Ijaz

et al. 2022

CIMB

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Phytohormones play an essential role in plant growth and development in response to environmental stresses. However, plant hormones require a complex signaling network combined with other signaling pathways to perform their proper functions. Thus, multiple phytohormonal signaling pathways are a prerequisite for understanding plant defense mechanism against stressful conditions. MicroRNAs (miRNAs) are master regulators of eukaryotic gene expression and are also influenced by a wide range of plant development events by suppressing their target genes. In recent decades, the mechanisms of phytohormone biosynthesis, signaling, pathways of miRNA biosynthesis and regulation were profoundly characterized. Recent findings have shown that miRNAs and plant hormones are integrated with the regulation of environmental stress. miRNAs target several components of phytohormone pathways, and plant hormones also regulate the expression of miRNAs or their target genes inversely. In this article, recent developments related to molecular linkages between miRNAs and phytohormones were reviewed, focusing on drought stress.

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“…Subsequently, the cassette was transferred into a pTF101.1gw1 vector and then used for transformation. The homozygous transgenic progenies used in this study were verified as independent lines according to Mendelian segregation analyses for the ratio of Basta-resistant/Basta-sensitive phenotypes, followed by molecular confirmation [96,97]. The plants were grown in plastic pots containing soil, coir, husk ash and compost (Tribat soil, Saigon Xanh Biotechnology Ltd. Company, Ho Chi Minh City, Vietnam) and under net house conditions (28-33 • C, 60-70% humidity and natural photoperiod) [12].…”

Section: Plant Materials and Plant Growth Conditionsmentioning

confidence: 99%

“…The primer sets used for this assay are provided in Table 1. Total RNA extraction and purification, cDNA synthesis and RT-qPCR were carried out using commercial kits (Thermo Scientific, Waltham, MA, USA) and following the guidelines provided by the manufacturer [97]. NanoDrop One C Microvolume UV-Vis Spectrophotometer (ND-ONEC-W, Thermo Scientific, MA, USA) was used to determine the concentrations and quality of total RNA extracts.…”

Section: Gene Expression Analysis By Rt-qpcrmentioning

confidence: 99%

“…cDNA synthesis was carried out using the same amount of total RNA from each sample. Preparation for reactions, thermal profile and melting curve analysis in RT-qPCR assay (Mastercycler®ep realplex, Eppendorf, Hamburg, Germany) was described in our previous study [97]. Fbox [107] was used as the reference gene for normalization based on the 2 −∆Ct method [108].…”

Section: Gene Expression Analysis By Rt-qpcrmentioning

confidence: 99%

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The Drought-Mediated Soybean GmNAC085 Functions as a Positive Regulator of Plant Response to Salinity

Hoang

Chuong

Hoa

et al. 2021

IJMS

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Abiotic stress factors, such as drought and salinity, are known to negatively affect plant growth and development. To cope with these adverse conditions, plants have utilized certain defense mechanisms involved in various aspects, including morphological, biochemical and molecular alterations. Particularly, a great deal of evidence for the biological importance of the plant-specific NAM, ATAF1/2, CUC2 (NAC) transcription factors (TFs) in plant adaptation to abiotic stress conditions has been reported. A previous in planta study conducted by our research group demonstrated that soybean (Glycine max) GmNAC085 mediated drought resistance in transgenic Arabidopsis plants. In this study, further characterization of GmNAC085 function in association with salt stress was performed. The findings revealed that under this condition, transgenic soybean plants overexpressing GmNAC085 displayed better germination rates than wild-type plants. In addition, biochemical and transcriptional analyses showed that the transgenic plants acquired a better defense system against salinity-induced oxidative stress, with higher activities of antioxidant enzymes responsible for scavenging hydrogen peroxide or superoxide radicals. Higher transcript levels of several key stress-responsive genes involved in the proline biosynthetic pathway, sodium ion transporter and accumulation of dehydrins were also observed, indicating better osmoprotection and more efficient ion regulation capacity in the transgenic lines. Taken together, these findings and our previous report indicate that GmNAC085 may play a role as a positive regulator in plant adaptation to drought and salinity conditions.

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Ectopic expression of GmHP08 enhances resistance of transgenic Arabidopsis toward drought stress

Cited by 8 publications

References 119 publications

Expression alteration analyses in the transgenic Arabidopsis carrying soybean Histidine-containing phosphotransmitter gene under salinity stress condition

Expression alteration analyses in the transgenic Arabidopsis carrying soybean Histidine-containing phosphotransmitter gene under salinity stress condition

Molecular Aspects of MicroRNAs and Phytohormonal Signaling in Response to Drought Stress: A Review

The Drought-Mediated Soybean GmNAC085 Functions as a Positive Regulator of Plant Response to Salinity

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