Ectopic Expression of a Self-Incompatibility Module Triggers Growth Arrest and Cell Death in Vegetative Cells

Lin, Zongcheng; Xie, Fei; Triviño, Marina Muñoz; Karimi, Mansour; Bosch, Marina; Franklin‐Tong, Vernonica E.; Nowack, Moritz K.

doi:10.1104/pp.20.00292

Cited by 22 publications

(28 citation statements)

References 65 publications

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“…Pollen ROS mediate [14] Osatg7-1 mediate [15] ROS mediate [16] PERSISTENT TAPETAL CELL2 mediate [17] AtLSD1 deathosome mediate [18] Female floral buds AMC9, MeGI, BAG5, AifA, and HSP70 mediate [19] Female gametophyte Auxin efflux [20] Fruit Ca 2+ -dependent nuclease [21] Double Fertilization ROS mediate [22] Vegetative cells PrpS-PrsS modulate PCD vegetative cells [23] Zygotic Embryogenesis - [24] Somatic Embryogenesis K + ATP channel activity [25] Phenol-storing cells [26] Embryonic Suspensor Cell - [27] Table 1.…”

Section: Plant Part Mechanism Referencementioning

confidence: 99%

Programmed Cell Death (PCD) in Plant: Molecular Mechanism, Regulation, and Cellular Dysfunction in Response to Development and Stress

Mondal¹,

Antony²,

Roy³

et al. 2022

Regulation and Dysfunction of Apoptosis

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Programmed cell death (PCD) or apoptosis is a genetically programmed cellular process. Though in the plant, a true caspase system is lacking, still PCD can occur throughout the life cycle at any cell type, tissue, and organ part in response to a wide range of stimuli. Here we have discussed the current understanding of plant PCD in terms of different pathways, cellular dysfunction, regulation, and signaling mechanisms. Our present study discussed how and to what extent PCD is involved in pre-zygotic and post-zygotic plant life cycle and emphasized to what extent PCD modulated in response to abiotic and biotic stress. Additionally, the expression profile of different PCD-associated genes that are modulated by developmental stage, biotic-abiotic stress, cellular metabolites are also elucidated. Hence, this study will be helpful for understanding the molecular and structural instincts of PCD in different stages of plant growth and development, response to biotic/abiotic stimuli, and cellular dysfunction.

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Section: Plant Part Mechanism Referencementioning

confidence: 99%

Programmed Cell Death (PCD) in Plant: Molecular Mechanism, Regulation, and Cellular Dysfunction in Response to Development and Stress

Mondal¹,

Antony²,

Roy³

et al. 2022

Regulation and Dysfunction of Apoptosis

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“…We recently established that expression of PrpS1-PrsS1 in Arabidopsis roots triggers an ectopic "SI-like PCD" response in vegetative tissues, resulting in root growth inhibition and death of root cells 22 . To test if HLD1 is necessary for this ectopic "SI-like PCD" response outside the reproductive context, we introduced hld1-c1, hld1-c2 and hld1-7 into the pUBQ10::PrpS1 transgenic line.…”

Section: Hld1 Regulates Ectopic "Si" In Arabidopsis Rootsmentioning

confidence: 99%

“…Plants were grown as described in (22). Briefly, chlorine gas sterilized seeds were sown out on LRC2 plates (2.15g.L -1 MS basal salts Duchefa Biochemie, 0.1g.L -1 MES, pH adjusted to 5.7 with KOH, 1.0% Plant Tissue Culture Agar NEOGEN), and stored in cold room (4 o C) for three days before being moved to a growth chamber for vertical growth with continuous light emitted by white fluorescent lamps (intensity 120 µmol.m 2 .s -1 ), at 22 o C. Seedlings were transferred to Jiffy pots in soil and grown under glasshouse conditions under a 16h light/8h dark regime at 22 o C. Seeds were collected when the plants were completely dry and kept at room temperature (RT) or 4 o C for long-term storage.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

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GPI anchor remodeling by the plant PGAP1 ortholog HLD1 is essential for Papaver self-incompatibility

Zhou

Xie

Triviño

et al. 2021

Preprint

Self Cite

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In eukaryotes, glycosylphosphatidylinositol anchored proteins (GPI-APs) are tethered to the outer leaflet of the plasma membrane where they function as key regulators of a plethora of biological processes. Self-incompatibility (SI) plays a pivotal role regulating fertilization in higher plants through recognition and rejection of self pollen. Here we used Arabidopsis thaliana lines engineered to be self-incompatible by expression of Papaver SI determinants for an SI suppressor screen. We identify HLD1, an ortholog of human GPI-inositol deacylase PGAP1, as a critical component involved in the SI response. Hld1 knockout plants had no obvious developmental defects, but SI was completely abolished. We show that HLD1 functions as a GPI-inositol deacylase and that this GPI-remodeling activity is essential for SI. Using GFP-SKU5 as a representative GPI-AP, the HLD1 mutation does not affect GPI-AP production and targeting, but alters the configuration of mature GPI-APs, which prevents GPI-AP release from the plasma membrane, suggesting that this process plays a critical role in the regulation of SI. Our data not only implicate a role for GPI-APs in SI, providing new directions to investigate SI mechanisms, but identify for the first time a function for GPI-AP remodeling and a role for inositol deacylation in plants.

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“…The effect of pollen inducing SI on stigma is similar to that of an external stress stimulator; reactive oxygen species (ROS) are thought to induce different types of PCD during development. It was found that pollen from different species or incompatibility accumulated a high level of H 2 O 2 when scattered on the stigma, but this level decreased significantly on the compatible stigma [16][17][18]. GAPC not only plays a role in energy supply, but also transmits the ROS signal in PLDδ interaction.…”

Section: Introductionmentioning

confidence: 99%

Analysis of SI-Related BoGAPDH Family Genes and Response of BoGAPC to SI Signal in Brassica oleracea L.

Xie

Zhang

et al. 2021

Genes

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Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is not only involved in carbohydrate metabolism, but also plays an important role in stress resistance. However, it has not been reported in Brassica oleracea. In this study, we performed a genome-wide identification of BoGAPDH in B. oleracea and performed cloning and expression analysis of one of the differentially expressed genes, BoGAPC. A total of 16 members of the BoGAPDH family were identified in B. oleracea, which were conserved, distributed unevenly on chromosomes and had tandem repeat genes. Most of the genes were down-regulated during self-pollination, and the highest expression was found in stigmas and sepals. Different transcriptome data showed that BoGAPDH genes were differentially expressed under stress, which was consistent with the results of qRT-PCR. We cloned and analyzed the differentially expressed gene BoGAPC and found that it was in the down-regulated mode 1 h after self-pollination, and the expression was the highest in the stigma, which was consistent with the result of GUS staining. The promoter region of the gene not only has stress response elements and plant hormone response elements, but also has a variety of specific elements for regulating floral organ development. Subcellular localization indicates that the BoGAPC protein is located in the cytoplasm and belongs to the active protein in the cytoplasm. The results of prokaryotic expression showed that the size of the BoGAPC protein was about 37 kDa, which was consistent with the expected results, indicating that the protein was induced in prokaryotic cells. The results of yeast two-hybrid and GST pull-down showed that the SRK kinase domain interacted with the BoGAPC protein. The above results suggest that the BoGAPDH family of B. oleracea plays an important role in the process of plant stress resistance, and the BoGAPC gene may be involved in the process of self-incompatibility in B. oleracea, which may respond to SI by encoding proteins directly interacting with SRK.

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Ectopic Expression of a Self-Incompatibility Module Triggers Growth Arrest and Cell Death in Vegetative Cells

Cited by 22 publications

References 65 publications

Programmed Cell Death (PCD) in Plant: Molecular Mechanism, Regulation, and Cellular Dysfunction in Response to Development and Stress

Programmed Cell Death (PCD) in Plant: Molecular Mechanism, Regulation, and Cellular Dysfunction in Response to Development and Stress

GPI anchor remodeling by the plant PGAP1 ortholog HLD1 is essential for Papaver self-incompatibility

Analysis of SI-Related BoGAPDH Family Genes and Response of BoGAPC to SI Signal in Brassica oleracea L.

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