Ectopic expression of a Catalpa bungei (Bignoniaceae) PISTILLATA homologue rescues the petal and stamen identities in Arabidopsis pi-1 mutant

Overexpression of Lilium formosanum MADS-box (LFMADS) Causing Floral Defects While Promoting Flowering in Arabidopsis thaliana, Whereas Only Affecting Floral Transition Time in Nicotiana tabacum

“…Our results, therefore, revealed that LFMADS2 may have the activity of B-class MADS-box transcription factor because the ectopic expression in Arabidopsis ( PI/pi background) turned the sepals into petaloid organs, although restoration of petals and stamens formation was not observed in this heterozygote pi-1 mutant with the overexpressed LFMADS2. This notion also has been observed in previous studies on PI homologue of other plant species, in which ectopic overexpression of other B-class MADS-box genes CabuPI led to the petaloid sepals change in transgenic Arabidopsis with PI / pi background [68]. …”

Expression Pattern and Functional Characterization of PISTILLATA Ortholog Associated With the Formation of Petaloid Sepals in Double-Flower Eriobotrya japonica (Rosaceae)

“…Amino acid sequences of these proteins, which contain the M, I, K, and C domains, were aligned using a ClustalW program (Thompson et al, 1994). A phylogenetic tree was constructed using MEGA 5.0 software (Kumar et al, 2008;Tamura et al, 2011) and the method described by Jing et al (2015). Parameters of the phylogenetic tree were the bootstrap of 1,000 replicates, substitution model of Jones-Taylor-Thornton (Jones et al, 1992), uniform rates, nearest neighbor interchange, and the complete deletion of gaps/missing data.…”

“…The qRT-PCR were assayed using the SYBR green I (Takara, Japan) and CFX96 Touch Real-time PCR Detection System (Bio-Rad, USA). The reaction mixture was cycled using the previous parameters described by Jing et al (2015). As an internal control, the E. japonica b-actin was used to normalize small differences in template amounts with the primers qEjactinF and qEjactinR (Shan et al, 2008).…”

“…The 35S::EjPI construct was transformed into heterozygous pi-1 mutant Arabidopsis lines via the Agrobacterium tumefaciens strain GV3101-90 using the floraldip method described by Clough and Bent (1998). The seeds of transgenic Arabidopsis lines were selected using the previous method described by Jing et al (2015). Then, the seedlings were transplanted in soil.…”

“…Therefore, functional diversification or conservation of PI orthologous genes need to be focused after the duplication events in different clades of angiosperms. The altered expressional patterns of PI orthologs in some angiosperm shaped floral organ diversification (Soltis et al, 2007;Viaene et al, 2009;Chen et al, 2012;Hofer et al, 2012;Sasaki et al, 2014;Jing et al, 2015;Dodsworth, 2017). However, the expression pattern and functional roles of PI ortholog in Eriobotrya remain unclear.…”