Ectopic application of the repressive histone modification H3K9me2 establishes post-zygotic reproductive isolation in<i>Arabidopsis thaliana</i>

Jiang, Hua; Moreno‐Romero, Jordi; Santos‐González, Juan; Jaeger, Geert De; Gevaert, Kris; Slijke, Eveline Van De; Köhler, Claudia

doi:10.1101/gad.299347.117

Cited by 66 publications

(68 citation statements)

References 102 publications

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“…However, our results differ in that AHL10 primarily affected growth at low w and in that AHL10 is a member of the less studied Clade B AHLs (AHL1-AHL14). The only other study of AHL10 that we are aware of found AHL10 to be involved in recruitment of the heterochromatin mark Histone 3 Lysine 9 dimethylation (H3K9me2) to ATrich transposable elements (53). We observed mis-regulated expression of several transposons in ahl10-1.…”

Section: The Growth Assays Gene Expression Analysis and Localizationmentioning

confidence: 62%

Phosphoproteomics of Arabidopsis Highly ABA-Induced1 identifies AT-Hook Like10 phosphorylation required for stress growth regulation

Wong

Bhaskara²,

Teng³

et al. 2018

Preprint

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The Clade A protein phosphatase 2C Highly ABA-Induced 1 (HAI1) plays an important role in stress signaling yet little information is available on HAI1-regulated phosphoproteins.Quantitative phosphoproteomics identified phosphopeptides of increased abundance in hai1-2 in unstressed plants and in plants exposed to low water potential (drought) stress. The identity and localization of the phosphoproteins as well as enrichment of specific phosphorylation motifs indicated that these phosphorylation sites may be regulated directly by HAI1 or by HAI1regulated kinases including Mitogen-Activated Protein Kinases (MPKs), Sucrose-non fermenting Related Kinase 2 (SnRK2s) or Casein Kinases. One of the phosphosites putatively regulated by HAI1 was S313/S314 of AT Hook-Like10 (AHL10), a DNA binding protein of unclear function.HAI1 could directly dephosphorylate AHL10 in vitro and the level of HAI1 expression affected the abundance of phosphorylated AHL10 in vivo. AHL10 S314 phosphorylation was critical for restriction of plant growth under low water potential stress and for regulation of Jasmonic Acid and Auxin-related gene expression as well as expression of developmental regulators including Shootmeristemless (STM). These genes were also mis-regulated in hai1-2. AHL10 S314 phosphorylation was required for AHL10 complexes to form foci within the nucleoplasm, suggesting that S314 phosphorylation may control AHL10 association with the nuclear matrix or with other transcriptional regulators. These data identify a set of HAI1-affected phosphorylation sites, show that HAI1-regulated phosphorylation of AHL10 S314 controls AHL10 function and localization and also indicate that HAI1-AHL10 signaling coordinates growth with stress and defense responses.

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Section: The Growth Assays Gene Expression Analysis and Localizationmentioning

confidence: 62%

Phosphoproteomics of Arabidopsis Highly ABA-Induced1 identifies AT-Hook Like10 phosphorylation required for stress growth regulation

Wong

Bhaskara²,

Teng³

et al. 2018

Preprint

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“…Alternatively, the doubling of the maternal genome may induce increased maternal loading of PRC2 and lead to a more repressed state of the target sites; paternal genome doubling may have caused titration PRC2 activity via increased copy numbers of the target sites (Kinoshita, ; Kohler et al ., ). In addition to the PRC2 and the type‐I MADS‐box pathway, recent analyses of an interploidy cross in A. thaliana revealed a second molecular pathway for the post‐zygotic hybridization barrier (Jiang et al ., ). In this pathway, the levels of H3K9me2 in the matrix attachment region are high in a cross of female 2x and male 4x A. thaliana ; these high levels are mediated by the paternally expressed ADMETOS gene, the AT‐hook domain protein AHL10 gene and the SUVH9 gene.…”

Section: Discussionmentioning

confidence: 97%

Overcoming the species hybridization barrier by ploidy manipulation in the genus Oryza

et al. 2018

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In most eudicot and monocot species, interspecific and interploidy crosses generally display abnormalities in the endosperm that are the major cause of a post-zygotic hybridization barrier. In some eudicot species, however, this type of hybridization barrier can be overcome by the manipulation of ploidy levels of one parental species, suggesting that the molecular mechanisms underlying the species hybridization barrier can be circumvented by genome dosage. We previously demonstrated that endosperm barriers in interspecific and interploidy crosses in the genus Oryza involve overlapping but different mechanisms. This result contrasts with those in the genus Arabidopsis, which shows similar outcomes in both interploidy and interspecific crosses. Therefore, we postulated that an exploration of pathways for overcoming the species hybridization barrier in Oryza endosperm, by manipulating the ploidy levels in one parental species, might provide novel insights into molecular mechanisms. We showed that fertile hybrid seeds could be produced by an interspecific cross of female tetraploid Oryza sativa and male diploid Oryza longistaminata. Although the rate of nuclear divisions did not return to normal levels in the hybrid endosperm, the timing of cellularization, nucellus degeneration and the accumulation of storage products were close to normal levels. In addition, the expression patterns of the imprinted gene MADS87 and YUCCA11 were changed when the species barrier was overcome. These results suggest that the regulatory machinery for developmental transitions and imprinted gene expression are likely to play a central role in overcoming species hybridization barriers by genome dosage in the genus Oryza.

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“…However, their level of deregulation remained substantially lower compared to 3x seeds. This data suggest that auxin acts either independently of the pathways previously shown to affect 3x seed abortion (Kradolfer et al, 2013a; Wolff et al, 2015; Jiang et al, 2017) or, alternatively, that auxin signalling is downstream of PEG and AGL functions in the endosperm.…”

Section: Resultsmentioning

confidence: 65%

Auxin regulates endosperm cellularization inArabidopsis

Figueiredo

Batista

Köhler

2017

Preprint

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9The endosperm is an ephemeral tissue that nourishes the developing embryo, similar to the 10 placenta in mammals. In most angiosperms endosperm development starts as a syncytium, where 11 nuclear divisions are not followed by cytokinesis. The timing of endosperm cellularization largely 12 varies between species and the event triggering this transition remains unknown. Here we show 13 that increased auxin biosynthesis in the endosperm prevents its cellularization, leading to seed 14 arrest. Auxin-overproducing seeds phenocopy paternal-excess triploid seeds derived from 15 hybridizations of diploid maternal plants with tetraploid fathers. Concurrently, auxin-related 16 genes are strongly overexpressed in triploid seeds, correlating with increased auxin activity. 17 Reducing auxin biosynthesis and signaling reestablishes endosperm cellularization in triploid 18 seeds and restores their viability, highlighting a causal role of increased auxin in preventing 19 endosperm cellularization. We propose that auxin determines the time of endosperm 20 cellularization and thereby uncovered a central role of auxin in establishing hybridization barriers 21 in plants.22

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Ectopic application of the repressive histone modification H3K9me2 establishes post-zygotic reproductive isolation inArabidopsis thaliana

Cited by 66 publications

References 102 publications

Phosphoproteomics of Arabidopsis Highly ABA-Induced1 identifies AT-Hook Like10 phosphorylation required for stress growth regulation

Phosphoproteomics of Arabidopsis Highly ABA-Induced1 identifies AT-Hook Like10 phosphorylation required for stress growth regulation

Overcoming the species hybridization barrier by ploidy manipulation in the genus Oryza

Auxin regulates endosperm cellularization inArabidopsis

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