Ectonucleotidases and adenosine deaminase activity in laying hens naturally infected by Salmonella Gallinarum and their effects on the pathogenesis of the disease

Boiago, Marcel M.; Baldissera, Matheus D.; Doleski, Pedro H.; Bottari, Nathieli B.; Carmo, Guilherme M. do; Araujo, Denise Nunes; Giuriatti, Jessica; Baggio, V.; Leal, Daniela Bitencourt Rosa; Casagrande, Renata Assis; Wisser, Claudia S.; Stefani, Lenita M.; Silva, Aleksandro S. Da

doi:10.1016/j.micpath.2016.02.008

Cited by 14 publications

(3 citation statements)

References 20 publications

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“…In the present study, a significant upregulation of splenic 5′‐nucleotidase may be interpreted as an anti‐inflammatory mechanism to rapidly hydrolyse AMP to form Ado, an anti‐inflammatory molecule (Bours et al., ), similarly to that observed by Boiago et al. () in laying hens experimentally infected with Salmonella Gallinarum. According to these authors, the upregulation of serum 5′‐nucleotidase contributed to restriction of the inflammatory response and subsequent cellular damage by increasing serum Ado levels.…”

Section: Discussionsupporting

confidence: 80%

“…Under pathological conditions, extracellular ATP increases in concentration and interacts with specific receptors (type P2 purine receptors) to induce the release of proinflammatory cytokines, making ATP the most important sensor of inflammation (Fredholm, ). Several lines of evidence have suggested that upregulation of NTPDase activity is associated with an attempt to hydrolyse excessive extracellular ATP levels, which may contribute to improvement of the immune response (Doleski et al., ; Boiago et al., ). Moreover, studies have demonstrated that upregulation of 5′‐nucleotidase activity is an attempt to increase the hydrolysis of AMP to form Ado, a potent immunomodulatory and immunosuppressor molecule (Di Virgilio & Vuerich, ).…”

Section: Introductionmentioning

confidence: 99%

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Purinergic signalling displays an anti‐inflammatory profile in the spleen of fish experimentally infected with Aeromonas caviae: Modulation of the immune response

Baldissera

Souza

Bottari

et al. 2017

Journal of Fish Diseases

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Extracellular adenosine triphosphate (ATP) and its metabolite adenosine (Ado) are recognized as key mediators of immune and inflammatory responses. Depending on its concentration, ATP may act as an immunostimulant or immunodepressant, while Ado levels display an anti-inflammatory profile. The aim of this study was to evaluate whether splenic purinergic signalling is capable of modulating immune and inflammatory responses in fish experimentally infected with Aeromonas caviae. Triphosphate diphosphohydrolase (NTPDase) and 5'-nucleotidase activities increased in the spleen of silver catfish (Rhamdia quelen) experimentally infected with A. caviae compared with the uninfected control group. Moreover, splenic Ado levels increased in the infected animals relative to the uninfected control group. Based on these lines of evidence, our findings revealed that adenine nucleotide hydrolysis is modified in the spleen of fish infected with A. caviae attempting to restrict the inflammatory process through the upregulation of NTPDase and 5'-nucleotidase activities, which occurs in an attempt to hydrolyse the excessive ATP in the extracellular environment and rapidly hydrolyse AMP to form Ado. In summary, purinergic signalling can modulate immune and inflammatory responses during A. caviae infection.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Purinergic signalling displays an anti‐inflammatory profile in the spleen of fish experimentally infected with Aeromonas caviae: Modulation of the immune response

Baldissera

Souza

Bottari

et al. 2017

Journal of Fish Diseases

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“…Moreover, this enzyme acts as an endogenous regulator of innate immunity, protecting the host tissue against excessive inflammatory damage (Frode & Medeiros, 2011). Recently, Boiago et al (2016) demonstrated that ADA activity could be interpreted as a marker to determine the severity of inflammatory damage during bacterial diseases, such as caused by Salmonella gallinarum. This author demonstrated that upregulation of ADA activity exerts a proinflammatory profile during salmonellosis infection, contributing to disease pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

Involvement of cholinergic and adenosinergic systems on the branchial immune response of experimentally infected silver catfish with Streptococcus agalactiae

Baldissera

Souza

Doleski

et al. 2017

Journal of Fish Diseases

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It has been recognized that the cholinergic and adenosinergic systems have an essential role in immune and inflammatory responses during bacterial fish pathogens, such as the enzymes acetylcholinesterase (AChE) and adenosine deaminase (ADA), which are responsible for catalysis of the anti-inflammatory molecules acetylcholine (ACh) and adenosine (Ado) respectively. Thus, the aim of this study was to investigate the involvement of the cholinergic and adenosinergic systems on the immune response and inflammatory process in gills of experimentally infected Rhamdia quelen with Streptococcus agalactiae. Acetylcholinesterase activity decreased, while ACh levels increased in gills of infected animals compared to uninfected animals. On the other hand, a significant increase in ADA activity with a concomitant decrease in Ado levels was observed in infected animals compared to uninfected animals. Based on this evidence, we concluded that infection by S. agalactiae in silver catfish alters the cholinergic and adenosinergic systems, suggesting the involvement of AChE and ADA activities on immune and inflammatory responses, regulating the ACh and Ado levels. In summary, the downregulation of AChE activity exerts an anti-inflammatory profile in an attempt to reduce or prevent the tissue damage, while the upregulation of ADA activity exerts a pro-inflammatory profile, contributing to disease pathophysiology.

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Purinergic signaling modulates the splenic inflammatory response in silver catfish naturally infected with Ichthyophthirius multifiliis

2018

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The spleen is an immune lymphatic organ linked with control of the immune response, which is important to fish health. Recent evidence has suggested the involvement of purinergic signaling in the modulation of immune and inflammatory responses through the nucleotide adenosine triphosphate (ATP) and the nucleoside adenosine (Ado), which are regulated by the enzymes nucleoside triphosphate diphosphohydrolase (NTPDase), 5'-nucleotidase, and adenosine deaminase (ADA). Thus, the aim of this study was to evaluate whether purinergic signaling can modulate the immune and inflammatory responses in the spleen of silver catfish (Rhamdia quelen) naturally infected with Ichthyophthirius multifiliis. Splenic NTPDase and 5'-nucleotidase activities increased in infected animals compared with uninfected animals, while the splenic ADA activity decreased. These data indicate that purinergic signaling can modulate the splenic immune and inflammatory responses through the regulation of ATP and Ado levels, which are known to participate in the physiological and pathological responses as pro-inflammatory and anti-inflammatory molecules, respectively. In summary, modulation of the splenic purinergic cascade has an anti-inflammatory role in reducing or preventing inflammatory damage.

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Ectonucleotidases and adenosine deaminase activity in laying hens naturally infected by Salmonella Gallinarum and their effects on the pathogenesis of the disease

Cited by 14 publications

References 20 publications

Purinergic signalling displays an anti‐inflammatory profile in the spleen of fish experimentally infected with Aeromonas caviae: Modulation of the immune response

Purinergic signalling displays an anti‐inflammatory profile in the spleen of fish experimentally infected with Aeromonas caviae: Modulation of the immune response

Involvement of cholinergic and adenosinergic systems on the branchial immune response of experimentally infected silver catfish with Streptococcus agalactiae

Purinergic signaling modulates the splenic inflammatory response in silver catfish naturally infected with Ichthyophthirius multifiliis

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