Ectomycorrhizal Fungi and Associated Bacteria Provide Protection Against Heavy Metals in Inoculated Pine (Pinus Sylvestris L.) Seedlings

Krupa, Piotr; Kozdrój, Jacek

doi:10.1007/s11270-006-9323-7

Cited by 52 publications

(18 citation statements)

References 33 publications

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“…Information on degrees of ECM colonization is lacking in most literature on the topic (Bradley et al 1981(Bradley et al , 1982Brown and Wilkins 1985;Bucking and Heyser 1994;Hartley-Whitaker et al 2000b;Adriaensen et al 2004Adriaensen et al , 2006Kim et al 2004;Krupa and Kozdroj 2007;Krznaric et al 2009). Where data are supplied, reported levels of infection are relatively low probably due to the usage of artificial media: e.g., 9-27.8 % (Jourand et al 2010), 22-49 % (Clint andDighton 1992), or highly variable; e.g., 0-75 % , 0-67 % (Dixon 1988), 13-62 % (Hartley-Whitaker et al 2000a), 0-93 % (Dixon and Buschena 1988), but 80-100 % (Colpaert and Van Assche 1992), 50-100 % (Jones and Hutchinson 1988b;Van Tichelen et al 2001).…”

Section: Discussionmentioning

confidence: 99%

“…Instead, experiments with plants growing in natural soil and with high infection ratios are required to advance understanding in this field. To date, few investigations have used plants grown in soils containing elevated levels of metals to study the mechanisms of metal tolerance in ECM trees (Dixon 1988;Dixon and Buschena 1988;Hartley-Whitaker et al 2000b;Krupa and Kozdroj 2007). Nevertheless, even though natural soils have been used in some cases, the roles of ECM fungi in the metal uptake and sensitivity of host plants have remained controversial.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, visible toxicity symptoms in trees usually appear after long periods of stress exposure (Adriaensen et al 2006). It is for this reason that previous studies using woody plants have been so protracted, e.g., 8 weeks (Hartley-Whitaker et al 2000a), 12 weeks (Bradley et al 1981(Bradley et al , 1982Dixon and Buschena 1988;Kozdroj et al 2007;Krupa and Kozdroj 2007;Jourand et al 2010), 16 weeks (Dixon 1988;Colpaert and van Assche 1992), 18 weeks , 6 months (Colpaert and van Assche 1993;Hartley-Whitaker et al 2000b;Zimmer et al 2009), 8 months (Kim et al 2004), and 9 months (Adriaensen et al 2006). A sensitive tool for rapid detection and monitoring of metal toxicity symptoms would promote progress in this field.…”

Section: Introductionmentioning

confidence: 99%

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Growth and photosynthetic responses of ectomycorrhizal pine seedlings exposed to elevated Cu in soils

et al. 2015

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It is still controversial whether ectomycorrhizal (ECM) mycelia filter out toxic metals in nutrient absorption of host trees. In this study, pine (Pinus densiflora) seedlings colonized by Cu-sensitive and Cu-tolerant ECM species were exposed to a wide spectrum of soil Cu concentrations to investigate functions of ECM fungi under Cu stress. The photosynthetic rates of intact needles were monitored in situ periodically. The biomass and elements of plants were also measured after harvest. The ameliorating effect of ECM infection on host plants exposed to toxic stress was metal concentration specific. Under lower-level Cu stress, ECM fungi increased seedling performance, while ECM seedlings accumulated more Cu than nonmycorrhizal (NM) seedlings. Under higher-level Cu stress, photosynthesis decreased well before visible symptoms of Cu toxicity appeared. The reduced photosynthesis and biomass in ECM seedlings compared to NM seedlings under higher Cu conditions were also accompanied by lower phosphorus in needles. There was no marked difference between the two fungal species. Our results indicate that the two ECM fungi studied in our system may not have an ability to selectively eliminate Cu in nutrient absorption and may not act as effective barriers that decrease toxic metal uptake into host plants.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Growth and photosynthetic responses of ectomycorrhizal pine seedlings exposed to elevated Cu in soils

et al. 2015

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“…The pine seedling inoculation with the mycorrhizal fungi, such as Scleroderma citrinum , Amanita muscaria and Lactarius rufus , revealed reduced translocation of Zn, Cd or Pb from roots to shoots compared with the controls. This effect was attributed to the increased metal biosorption by outer and inner components of the mycelium (Krupa and Kozdrój 2007 ). The fungal cell wall components (e.g.…”

Section: Biomass and Biological Macromolecule-mediated Remediationmentioning

confidence: 99%

Remediation of Heavy Metal-Contaminated Agricultural Soils Using Microbes

Singh

Mishra

et al. 2016

Microbial Inoculants in Sustainable Agricultural Productivity

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Heavy metals are widely spread and accumulated in soil due to various inappropriate human activities, because of which metal pollution in soil has become one of the most serious environmental problems today. In this chapter, various microbial remediation mechanisms to remediate heavy metal-contaminated soils have been described. Microbial remediation, an emerging cost-effective, renewable, nonintrusive and aesthetically pleasing technology, uses the remarkable ability of microbes to remove and transform heavy metals from contaminated soils. The very limited understanding pertaining to heavy metal removal and transformation is hindering its effective application. Due to its great potential as a viable alternative to conventional contaminated soil remediation techniques, microbial remediation is currently being looked upon as an exciting area of basic and applied research.

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“…This is well documented in wooden plants that go into the mutualistic mycorrhizal symbiosis and produce ectomycorrizal roots which preserve, store and exchange mineral salts and water [9]. It has been shown that this type of plant association with fungi significantly enhance their tolerance to toxic concentration of heavy metals in soil [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Adaptation of Selected Ectomycorrhizal Fungi to Increased Concentration of Cadmium and Lead

Bandurska

Krupa

Berdowska

et al. 2016

Ecological Chemistry and Engineering S

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Plants together with water and minerals actively take from the soil heavy metals such as cadmium and lead. The negative role of ions of these metals on plant growth and development depends not only on their concentration in the soil, but also on a number of factors that may affect the transport of minerals from the soil to the roots. The harmful effects of xenobiotics getting from the soil to the plants are limited by the organic compounds contained in the soil, soil structure and pH. Particularly noteworthy are biotic factors, such as bacteria and fungi which greatly limit the translocation of heavy metals. Stream of new scientific reports show that the symbiotic combination of fungi with plant roots so called mycorrhizae is a factor that may be important in reducing the impact of soil contamination by heavy metals. Mycorrhiza by filtering solutions of water and mineral salts stop a considerable amount of heavy metals in the internal mycelium or on its surface. It was proved that plants with properly formed mycorrhiza grow better in hard to renew lands, such as salty, sterile soils contaminated with industrial waste. Questions to which answer was sought in this study are: 1) whether mycorrhizal fungi for many years growing in the contaminated areas have managed to adapt to these adverse conditions and 2) do the same species derived from clean areas are less resistant to contamination by heavy metals? Stated problems tried to be solved based on the fruiting bodies of fungi collected from ectomycorrhizal fungi picked from the areas contaminated by industrial emissions and areas free of contamination. The interaction of cadmium and lead ions on the growth of mycelium was examined by plate method and binding of heavy metals in fruiting structures of fungi were done by colorimetric method with use of methylene blue. It has been shown that the fungal resistance, even of the same species, to high concentration of heavy metals varies depending on the origin of symbiont. Isolated fungi from contaminated areas are better adapted to high concentrations of xenobiotics. Ability to bind cadmium and lead to fruiting bodies of fungi varies.

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Ectomycorrhizal Fungi and Associated Bacteria Provide Protection Against Heavy Metals in Inoculated Pine (Pinus Sylvestris L.) Seedlings

Cited by 52 publications

References 33 publications

Growth and photosynthetic responses of ectomycorrhizal pine seedlings exposed to elevated Cu in soils

Growth and photosynthetic responses of ectomycorrhizal pine seedlings exposed to elevated Cu in soils

Remediation of Heavy Metal-Contaminated Agricultural Soils Using Microbes

Adaptation of Selected Ectomycorrhizal Fungi to Increased Concentration of Cadmium and Lead

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