Ectoine: a compatible solute in radio‐halophilic<i>Stenotrophomonas</i>sp.<scp>WMA</scp>‐<scp>LM</scp>19 strain to prevent ultraviolet‐induced protein damage

Sajjad, Wasim; Qadir, Syed Abdul; Ahmad, Manzoor; Rafiq, Muhammad; Hasan, Fariha; Tehan, Richard M.; McPhail, Kerry L.; Shah, Aamer Ali

doi:10.1111/jam.13903

Cited by 18 publications

(9 citation statements)

References 39 publications

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“…5 Ectoine has a zwitterionic structure in the solid state 6 and in aqueous solution 7,8 at neutral pH. Its water structuring (kosmotropic) effects, 5,[7][8][9] role as a hydroxyl radical scavenger, 10,11 and influence on protein functions, 9,[12][13][14][15][16] gene expression, 17,18 DNA melting temperature, 12,19 DNA structure, 20,21 DNA-protein binding, 9,12 and protection of biomolecules against ionising radiation 10 and oxidative stress 22 were investigated over the last decade. Due to its versatile properties, ectoine is currently used in a multitude of biotechnological, cosmetic and medical applications.…”

Section: Introductionmentioning

confidence: 99%

“…Due to its versatile properties, ectoine is currently used in a multitude of biotechnological, cosmetic and medical applications. 2,3,[22][23][24][25] Most prominently, it is used in sunscreens, despite evidence from recent studies concerning its influence on UV irradiation of biological targets, showing Janus-faced behaviour. 17,20,[26][27][28] Bünger et al irradiated human keratinocytes with UV-A (340-400 nm).…”

Section: Introductionmentioning

confidence: 99%

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Ectoine interaction with DNA: influence on ultraviolet radiation damage

Hahn

Smales

Seitz

et al. 2020

Phys. Chem. Chem. Phys.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ectoine interaction with DNA: influence on ultraviolet radiation damage

Hahn

Smales

Seitz

et al. 2020

Phys. Chem. Chem. Phys.

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“…Specifically, they are usually able to express enzymes capable of repairing the damaged DNA created by irradiation and thus are able to survive high levels of radiation. Specific chemicals, such as ectoine from Stenotrophomonas spp., have been shown to overcome radiation-mediated oxidative damage in an extreme UV irradiation environment (Sajjad et al 2018 ).…”

Section: Discussionmentioning

confidence: 99%

A coupled UV photolysis-biodegradation process for the treatment of decabrominated diphenyl ethers in an aerobic novel bioslurry reactor

Chang

Chen

Chou

et al. 2020

Environ Sci Pollut Res

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The commercial flame retardant is an emerging contaminant (EC) commonly found in soils and sediments. A coupled UVphotolysis-biodegradation process was used to decompose decabromodiphenyl ether (BDE-209) in clay slurries. A novel bioslurry bioreactor (NBB) was employed in which BDE-209 degradation was maximized by the simultaneous application of LED UVA irradiation and biodegradation by a mixed bacterial culture. The rate of BDE-209 degradation decreased in the order: coupled UV photolysis-biodegradation (1.31 × 10-2 day-1) > UV photolysis alone (1.10 × 10-2 day-1) > biodegradation alone (1.00 × 10-2 day-1). Degradation intermediates detected included hydroxylated polybrominated diphenylethers, partially debrominated PBDE congeners and polybrominated dibenzofuran. The UV-resistant bacterial strains isolated that could utilize BDE-209 as a sole carbon source included Stenotrophomonas sp., Pseudomonas sp., and Microbacterium sp. These strains encoded important functional genes such as dioxygenase and reductive dehalogenases. Continuous UV irradiation during the NBB process affected various biochemical oxidative reactions during PBDEs biodegradation. Simultaneous photolysis and biodegradation in the NBB system described reduces operational time, energy, expense, and maintenance-demands required for the remediation of BDE-209 when compared to sequential UV-biodegradation process or to biodegradation alone. Keywords Coupled UV photolysis-biodegradation. Decabromodiphenyl ether. Novel bioslurry bioreactor. UV-resistant bacterial strains. Polybrominated dibenzofuran Highlights • A time, energy, and cost-saving technique using a coupled UV photolysis-biodegradation process for degrading BDE-209 in a novel bioslurry bioreactor. • Identification of isolated UV-resistant bacterial strains that potentially are able to utilize PBDEs as sole carbon source and the analysis of related functional genes. • The prediction of a possible pathway for BDE-209 degradation by coupled photolysis-biodegradation.

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“…It stabilizes cell membranes, enzymes, and nucleic acids at extreme temperatures or higher salt concentrations in some bacteria (Smiatek et al ., 2012). Furthermore it absorbs UV-A radiation, preserves DNA from breaking down in various cell types and reduce oxidative stress caused by UV (Buenger and Driller, 2004; Botta et al ., 2008; Sajjad et al ., 2018). It is remarkable that SS were singular in regard to his significant presence of ectoine synthase, since this fact is compatible with the stressful environment in which it was sampled, including high salt concentration in the close hypersaline Socompa lake (17%) and high exposure to UV irradiation leading to high osmotic and oxidative stress respectively.…”

Section: Discussionmentioning

confidence: 99%

Uncovering Photolyase/Cryptochrome Genes Diversity in Aquatic Microbiomes Exposed to Diverse UV-B Regimes

Alonso-Reyes

Farı́as

Albarracín

2019

Preprint

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During evolution, microorganisms exposed to high UV-B doses developed a fine-tuned photo-enzymes called “photolyases” to cope with DNA damage by UV-B. These photoreceptors belonging to the Cryptochrome/Photolyase Family (CPF) were well characterized at the genomic and proteomic level in bacteria isolated from a wide range of environments. In this work, we go further towards studying the abundance of CPF on aquatic microbial communities from different geographic regions across the globe. Metagenomics data combined with geo-referenced solar irradiation measurements indicated that the higher the UV-B dose suffered by the microbiome’s environment, the higher the abundance of CPF genes and lower the microbial diversity. A connection between CPF abundance and radiation intensity/photoperiod was reported. Likewise, cryptochrome-like genes were found abundant in most exposed microbiomes, indicating a complementary role to standard photolyases. Also, we observed that CPFs are more likely present in dominant taxa of the highly irradiated microbiomes, suggesting an evolutionary force for survival and dominance under extreme solar exposure. Finally, this work reported three novel CPF clades not identified so far, proving the potential of global metagenomic analyses in detecting novel proteins.

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Ectoine: a compatible solute in radio‐halophilicStenotrophomonassp.WMA‐LM19 strain to prevent ultraviolet‐induced protein damage

Abstract: Due to its anti-oxidant properties, ectoine from a radio-halophilic bacterium might be used in sunscreen formulation for protection against UV-induced oxidative stress.

Cited by 18 publications

References 39 publications

Ectoine interaction with DNA: influence on ultraviolet radiation damage

Ectoine interaction with DNA: influence on ultraviolet radiation damage

A coupled UV photolysis-biodegradation process for the treatment of decabrominated diphenyl ethers in an aerobic novel bioslurry reactor

Uncovering Photolyase/Cryptochrome Genes Diversity in Aquatic Microbiomes Exposed to Diverse UV-B Regimes

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