ECRG2, a novel candidate of tumor suppressor gene in the esophageal carcinoma, interacts directly with metallothionein 2A and links to apoptosis

Cui, Yongping; Wang, Jianbo; Zhang, Xin Yu; Lang, Ronggang; Bi, Meixia; Guo, Liping; Lu, Shih Hsin

doi:10.1016/s0006-291x(03)00122-0

Cited by 50 publications

(55 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4,5 Short tandem repeat polymorphism of ECRG2 in exon 4 was found to be significantly associated with esophageal squamous cell carcinoma, oral squamous carcinoma and pancreatic carcinoma. [12][13][14] These lines of evidence suggest ECRG2 has an important role in the process of carcinogenesis and is a novel candidate tumor-suppressor gene.…”

Section: Discussionmentioning

confidence: 99%

“…ECRG2 has been cloned in 1998 by mRNA differential display by comparing the gene expression between normal esophageal epithelia and esophageal carcinoma 9 and further studies showed that it had roles as a tumor suppressor. [4][5][6]10 It was downregulated in esophageal squamous carcinoma and other cancers. ECRG2 is important for ensuring centrosome duplication and accurate chromosome segregation, and its disruption leads to centrosome amplification and spindle checkpoint defects, contributing chromosome instability.…”

Section: Discussionmentioning

confidence: 99%

“…3 Cui et al have cloned a gene esophageal cancer-related gene 2 (ECRG2), which is downregulated in esophageal carcinomas by mRNA differential display technique. 4 It was mapped to human chromosome 5q32-33 and encodes a protein of 85 amino acids. 5 It was highly expressed in a series of normal tissues but was downregulated in the cancerous and adjacent tissues.…”

Section: Introductionmentioning

confidence: 99%

“…5 It was highly expressed in a series of normal tissues but was downregulated in the cancerous and adjacent tissues. 5 Cui et al 4 showed that ECRG2 interacted with MT2A physically and induced apoptosis of cancer cells. Further, Huang et al 6 reported that ECRG2 inhibited invasion and metastasis of lung cancer cell by suppressing urokinase-type plasmin activator/plasmin pathway.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Suppression of hepatocarcinoma model in vitro and in vivo by ECRG2 delivery using adenoviral vector

Song

Wang

et al. 2012

Cancer Gene Ther

View full text Add to dashboard Cite

Hepatocarcinoma represents one of the most malignant cancer types. Esophageal cancer-related gene 2 (ECRG2) is found to be critical in the process of carcinogenesis. It regulates urokinase-type plasmin activator receptor and extracellular matrix function and its polymorphism in exon 4 is associated with cancer relapse. To explore new strategies to fight against cancer, here we first systematically evaluated the therapeutic potential as a biological tool using adenoviral vector (Ad-ECRG2). Ad-ECRG2 is exogenously expressed in cytoplasm and is potent to suppress the growth of cancer cell by inducing apoptosis as effective as Ad-p53. Ad-ECRG2 is able to suppress the invasion and adhesion of cancer cells at low titers. It alters the expression of a panel of cancer-related molecules, including nuclear factor-kB, matrix metalloproteinase 2 and E-cadherin, contributing to reverse malignancy phenotype of cancer cells. In vivo experiments show a significant inhibition of cancer growth by intratumoral Ad-ECRG2 administration. No evident toxicity was observed in the model animal during the study. We concluded that ECRG2 is a potential molecular target in biological therapy strategies for cancer treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Suppression of hepatocarcinoma model in vitro and in vivo by ECRG2 delivery using adenoviral vector

Song

Wang

et al. 2012

Cancer Gene Ther

View full text Add to dashboard Cite

show abstract

“…Esophageal cancerrelated gene 2 (ECRG2), also named serine peptidase inhibitor Kazal-type 7 (SPINK7), was identified as a growth-regulating gene by comparing normal esophageal epithelia and primary squamous cell carcinomas tissues from high incidence families in Lin-Xian County of China using differentially displayed PCR (Su et al 1998;Cui et al 2010). It has been shown that ECRG2 is a tumor suppressor gene involved in the regulation of cell proliferation, induction of apoptosis, and inhibition of cancer cell migration, invasion and metastasis (Cui et al 2003;Huang et al 2007). ECRG2 also participates in centrosome amplification in a p53-dependent manner and has a role in maintaining chromosome stability (Cheng et al 2008).…”

Section: Biological Contextmentioning

confidence: 99%

NMR structure note: human esophageal cancer-related gene 2

et al. 2012

View full text Add to dashboard Cite

Short tandem repeat polymorphism in a novel esophageal cancer‐related gene (ECRG2) implicates susceptibility to esophageal cancer in Chinese population

Yue

Tan

et al. 2003

Intl Journal of Cancer

Self Cite

View full text Add to dashboard Cite

We have previously cloned and identified a novel esophageal cancer related gene 2 (ECRG2; GenBank Accession Number AF268198), which is down-regulated in esophageal squamous cell carcinoma (ESCC) and involved in the induction of the apoptosis in esophageal cancer cell lines. In the present study, we have found a short tandem repeat (STR) polymorphism in the noncoding region of the exon 4 of the ECRG2 gene by using PCR-denaturing high-performance liquid chromatography (DHPLC Esophageal cancer, with a 5-year survival rate Ͻ10%, is one of the most common fetal cancers worldwide and occurs at very high frequencies in certain areas of China, Iran, South Africa, Uruguay, France and Italy. 1 Fifty percent of esophageal cancer cases in the world occur in China. Among high-risk areas of esophageal squamous cell carcinoma (ESCC), Linxian County in Henan Province has the highest age-adjusted mortality rate of this cancer. 2 Epidemiological studies have revealed that the incidence of ESCC is associated with several factors, such as N-nitrosamines, which have been shown to be involved in the etiology of ESCC in Linxian. 3 However, studies in high-risk areas have demonstrated a strong tendency towards familial aggregation or clustering of cases within families, suggesting that genetic susceptibility factors may also play an important role in the etiology of ESCC.Previous studies have shown that several genetic abnormalities including amplification of C-myc, Int-2 and Hst, 4,5 mutation and/or deletion of p53 and Rb 2 and allelic deletion 6 have frequently occurred in human ESCC and esophageal cancer cell lines. However, the genetic events that lead to the development of esophageal cancer are not clear yet. In recent years, many studies of esophageal cancer are focused on the cloning and identification of novel esophageal cancer-related genes, which might play an important role in the carcinogenesis and development of the cancer. [7][8][9] Recently, we have cloned and identified a novel ESCC-related gene, ECRG2 (Genbank Accession Number AF 268198), from human normal esophageal epithelia. Firstly, we obtained an EST fragment by mRNA differential display techniques from 3 normal esophageal epithelia and 2 primary ESCC tissues from high incidence families in Linxian County and named it ECRG2. The RT-PCR detection results demonstrated that the expression of ECRG2 was down-regulated in ESCC as well as colon and brain tumors. 10 Secondly, we obtained the 569 bp full-length cDNA of the ECRG2 gene by SMARTTM RACE technique. We found that the gene contains 4 exons, spans about 3,540 bp on chromosome 5q32-33 and has a 258 bp open reading frame encoding an 85-amino acids polypeptide. 11 Analysis by bioinformatics has shown that 97% of the amino acid sequences of ECRG2 are homologous to a tumor associated KAZAL-type serine protease inhibitor that may play a central role in the protection of esophageal mucosa. 12 Thirdly, we studied the function of the ECRG2 gene by yeast 2-hybrid system and identified that the ECRG2 gene interacts directly ...

show abstract

ECRG2, a novel candidate of tumor suppressor gene in the esophageal carcinoma, interacts directly with metallothionein 2A and links to apoptosis

Cited by 50 publications

References 24 publications

Suppression of hepatocarcinoma model in vitro and in vivo by ECRG2 delivery using adenoviral vector

Suppression of hepatocarcinoma model in vitro and in vivo by ECRG2 delivery using adenoviral vector

NMR structure note: human esophageal cancer-related gene 2

Short tandem repeat polymorphism in a novel esophageal cancer‐related gene (ECRG2) implicates susceptibility to esophageal cancer in Chinese population

Contact Info

Product

Resources

About