ECR-MAPK Regulation in Liver Early Development

Abstract

Early growth is connected to a key link between embryonic development and aging. In this paper, liver gene expression profiles were assayed at postnatal day 22 and week 16 of age. Meanwhile another independent animal experiment and cell culture were carried out for validation. Significance analysis of microarrays, qPCR verification, drug induction/inhibition assays, and metabonomics indicated that alpha-2u globulin (extracellular region)-socs2 (-SH2-containing signals/receptor tyrosine kinases)-ppp2r2a/pik3c3 (MAPK signaling)-hsd3b5/cav2 (metabolism/organization) plays a vital role in early development. Taken together, early development of male rats is ECR and MAPK-mediated coordination of cancer-like growth and negative regulations. Our data represent the first comprehensive description of early individual development, which could be a valuable basis for understanding the functioning of the gene interaction network of infant development.

Identification of potential key genes associated with termination phase of rat liver regeneration through microarray analysis

“…This may be the reason for the increased expression of the gene in the late phase of liver regeneration. A study conducted by Zhao et al [29] showed that tumor-like growth in the early phase of liver development is positively and negatively coordinated by α-2u globulin (extracellular domain)/ppp2r2a-pik3c3 (MAPK signaling pathway)/Hsd3b5 (metabolic pathway), evidently showing that Hsd3b5 gene plays an important role in the biosynthesis of all steroid hormones. In addition, another article by Xiu et al [30] also showed a significant expression decrease in Hsd3b5 after lowering serum testosterone and estrogen.…”

Intervention of pumpkin seed oil on metabolic disease revealed by metabonomics and transcript profile

“…Research on the relationship between PSO dietary intervention and the occurrence and development of MD is still in the preliminary stage of observing phenomena, so the mechanism of action whereby PSO intervention alleviates MD is largely unclear. The aim of this study was to establish the MD metabolic profile and elucidate the variation law of the migration process of PSO intervention in alleviating MD, using nuclear magnetic resonance (NMR)‐based metabonomics combined with quantitative real‐time polymerase chain reaction (qPCR) to analyze lipid uptake and transport and the gene expression and protein levels of ERS, TLR (toll‐like receptor), UPR and inflammation pathways. Based on the high homology between humans and rats, the results of this study will provide basic information and reference data on MD prevention through PSO dietary intervention in the human population.…”