ECPGR recommended SSR loci for analyses of European plum (Prunus domestica) collections

Nybom, Hilde; Giovannini, Daniela; Ordidge, Matthew; Hjeltnes, S.H.; Grahić, Jasmin; Gaši, Fuad

doi:10.46265/genresj.2020.1.40-48

Cited by 8 publications

(18 citation statements)

References 37 publications

(50 reference statements)

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“…Hazelnut germplasm collections were analyzed in different world areas through biochemical [ 51 , 52 , 53 ], morphological, and genetic characterization using various molecular markers, among which SSRs are the most representative [ 28 , 34 , 38 , 54 ]. The development of molecular markers provided increasingly usable tools for DNA-based signature of tree crop varieties [ 55 , 56 , 57 ]. Here, nine SSR loci, standardized and recommended as replicable DNA-based markers by Biodiversity International [ 55 , 58 ], were employed to investigate the genetic diversity of Sicilian hazelnut germplasm cultivated in the Nebrodi Mountains, making possible the identification of varieties.…”

Section: Discussionmentioning

confidence: 99%

Diversity Assessment and DNA-Based Fingerprinting of Sicilian Hazelnut (Corylus avellana L.) Germplasm

Fiore

Marchese

Mauceri

et al. 2022

Plants

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The characterization of plant genetic resources is a precondition for genetic improvement and germplasm management. The increasing use of molecular markers for DNA-based genotype signature is crucial for variety identification and traceability in the food supply chain. We collected 75 Sicilian hazelnut accessions from private and public field collections, including widely grown varieties from the Nebrodi Mountains in north east Sicily (Italy). The germplasm was fingerprinted through nine standardized microsatellites (SSR) for hazelnut identification to evaluate the genetic diversity of the collected accessions, validating SSR discrimination power. We identified cases of homonymy and synonymy among acquisitions and the unique profiles. The genetic relationships illustrated by hierarchical clustering, structure, and discriminant analyses revealed a clear distinction between local and commercial varieties. The comparative genetic analysis also showed that the Nebrodi genotypes are significantly different from the Northern Italian, Iberian, and Turkish genotypes. These results highlight the need and urgency to preserve Nebrodi germplasm as a useful and valuable source for traits of interest employable for breeding. Our study demonstrates the usefulness of molecular marker analysis to select a reference germplasm collection of Sicilian hazelnut varieties and to implement certified plants’ production in the supply chain.

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Section: Discussionmentioning

confidence: 99%

Diversity Assessment and DNA-Based Fingerprinting of Sicilian Hazelnut (Corylus avellana L.) Germplasm

Fiore

Marchese

Mauceri

et al. 2022

Plants

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“…See Table 2 for diploid plum (P. salicina and related interspecific hybrids). The reference genotype is "Stanley" (from Nybom et al, 2020); (a) the REVERSE-PRIMER reported in Nybom et al (2020) for the marker UDP96-005 is not correct. For all SSR loci, the linkage group (LG) was reported in accordance with the GDR database and…”

Section: 36mentioning

confidence: 99%

SSR‐based DNA fingerprinting of fruit crops

et al. 2023

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The DNA fingerprinting of fruit crops, based on DNA microsatellite markers that are considered to be the key markers for the molecular analysis of germplasm collections, is reviewed. Simple sequence repeats (SSRs) remain the markers of choice for fingerprinting in humans, animals, plants, and other living organisms. This review, that considers 44 fruit species, provides a set of markers that are suitable for profiling accessions and that should make the databases produced in different laboratories more comparable. Every effort has been made to select SSR markers that are robust and easily scorable considering that such analyses are sometimes used in legal cases. The review first describes the basic protocols, procedures, and methods of data analyses; it then describes the fingerprinting of individual species or groups of species, providing a set of SSR markers and appropriate guidance based on the revised literature and on the authors' experience.

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“…Within these projects, morphological and genetic data were collected and analyzed on 104 accessions of local plums, conserved in 14 different European countries [49,86], and further extended to 165 accessions [87].…”

Section: Plum Varietal Diversitymentioning

confidence: 99%

Unlocking Plum Genetic Potential: Where Are We At?

et al. 2022

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Plums are a large group of closely related stone fruit species and hybrids of worldwide economic importance and diffusion. This review deals with the main aspects concerning plum agrobiodiversity and its relationship with current and potential contributions offered by breeding in enhancing plum varieties. The most recent breeding achievements are revised according to updated information proceeding from relevant scientific reports and official inventories of plum genetic resources. A special emphasis has been given to the potential sources of genetic traits of interest for breeding programs as well as to the need for efficient and coordinated efforts aimed at efficaciously preserving the rich and underexploited extant plum agrobiodiversity. The specific objective of this review was to: (i) analyze and possibly evaluate the degree of biodiversity existing in the cultivated plum germplasm, (ii) examine the set of traits of prominent agronomic and pomological interest currently targeted by the breeders, and (iii) determine how and to what extent this germplasm was appropriately exploited in breeding programs or could represent concrete prospects for the future.

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ECPGR recommended SSR loci for analyses of European plum (Prunus domestica) collections

Cited by 8 publications

References 37 publications

Diversity Assessment and DNA-Based Fingerprinting of Sicilian Hazelnut (Corylus avellana L.) Germplasm

Diversity Assessment and DNA-Based Fingerprinting of Sicilian Hazelnut (Corylus avellana L.) Germplasm

SSR‐based DNA fingerprinting of fruit crops

Unlocking Plum Genetic Potential: Where Are We At?

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