Ecotoxicity of different-shaped silver nanoparticles: Case of zebrafish embryos

Abramenko, Natalia; Демидова, Т. Б.; Abkhalimov, E. V.; Ершов, Б. Г.; Krysanov, E. Yu.; Kustov, L. М.

doi:10.1016/j.jhazmat.2017.12.060

Cited by 101 publications

(57 citation statements)

References 31 publications

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“…The zebrafish chorion has pores of 0.5-0.7 µm diameter [11,31], which are necessary for oxygen and nutrient transportation for the embryos [32]. Arguably, the AgNPs entering the chorion represent an opportunity for uptake of AgNPs in the embryo tissues [12,18,33] or for later development abnormalities [11,13]. Compared to S10 AgNPs, S4 AgNPs with a smaller size have a larger surface area that can enhance their adherence to the embryo chorion, which may contribute to the developmental toxicity of AgNPs by blocking the chorion pores and thus obstruct the oxygen exchange.…”

Section: Discussionmentioning

confidence: 99%

Silver Nanoparticles in Zebrafish (Danio rerio) Embryos: Uptake, Growth and Molecular Responses

Qiang

Arabeyyat

et al. 2020

IJMS

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Silver nanoparticles (AgNPs) are widely used in commercial applications as antimicrobial agents, but there have recently been increasing concerns raised about their possible environmental and health impacts. In this study, zebrafish embryos were exposed to two sizes of AgNP, 4 and 10 nm, through a continuous exposure from 4 to 96 h post-fertilisation (hpf), to study their uptake, impact and molecular defense responses. Results showed that zebrafish embryos were significantly impacted by 72 hpf when continuously exposed to 4 nm AgNPs. At concentrations above 0.963 mg/L, significant in vivo uptake and delayed yolk sac absorption was evident; at 1.925 mg/L, significantly reduced body length was recorded compared to control embryos. Additionally, 4 nm AgNP treatment at the same concentration resulted in significantly upregulated hypoxia inducible factor 4 (HIF4) and peroxisomal membrane protein 2 (Pxmp2) mRNA expression in exposed embryos 96 hpf. In contrast, no significant differences in terms of larvae body length, yolk sac absorption or gene expression levels were observed following exposure to 10 nm AgNPs. These results demonstrated that S4 AgNPs are available for uptake, inducing developmental (measured as body length and yolk sac area) and transcriptional (specifically HIF4 and Pxmp2) perturbations in developing embryos. This study suggests the importance of particle size as one possible factor in determining the developmental toxicity of AgNPs in fish embryos.

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Section: Discussionmentioning

confidence: 99%

Silver Nanoparticles in Zebrafish (Danio rerio) Embryos: Uptake, Growth and Molecular Responses

Qiang

Arabeyyat

et al. 2020

IJMS

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“…As a result, various studies have been designed to assess the effect of even low doses of silver nanoparticles (AgNPs) on bacterial cell membranes [ 30 , 31 , 32 ]. Results on in vivo toxicity of AgNPs have been reported for various living organisms, such as zebrafish [ 33 , 34 ], catfish [ 35 ], common carp [ 36 ], Corbicula fluminea clam [ 37 ], Caenorhabditis elegans worm [ 38 ], and mice [ 39 , 40 ]. The interaction of metal nanoparticles with nucleic acids is also a hot topic in the field of bioinorganic research, due to the interest in elucidating possible effects of Ag nanoparticles on the synthesis, replication, and structural integrity of DNA and RNA [ 41 ] and the mechanism of DNA–nanoparticle binding [ 42 ].…”

Section: Introductionmentioning

confidence: 99%

Chitosan-Stabilized Ag Nanoparticles with Superior Biocompatibility and Their Synergistic Antibacterial Effect in Mixtures with Essential Oils

et al. 2018

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Silver nanoparticles (AgNPs) are considered a promising alternative to the use of antibiotics in fighting multidrug-resistant pathogens. However, their use in medical application is hindered by the public concern regarding the toxicity of metallic nanoparticles. In this study, rationally designed AgNP were produced, in order to balance the antibacterial activity and toxicity. A facile, environmentally friendly synthesis was used for the electrochemical fabrication of AgNPs. Chitosan was employed as the capping agent, both for the stabilization and to improve the biocompatibility. Size, morphology, composition, capping layer, and stability of the synthesized nanoparticles were characterized. The in vitro biocompatibility and antimicrobial activities of AgNPs against common Gram-negative and Gram-positive bacteria were evaluated. The results revealed that chitosan-stabilized AgNPs were nontoxic to normal fibroblasts, even at high concentrations, compared to bare nanoparticles, while significant antibacterial activity was recorded. The silver colloidal dispersion was further mixed with essential oils (EO) to increase the biological activity. Synergistic effects at some AgNP–EO ratios were observed, as demonstrated by the fractionary inhibitory concentration values. Our results reveal that the synergistic action of both polymer-stabilized AgNPs and essential oils could provide a significant efficiency against a large variety of microorganisms, with minimal side effects.

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“…The high rate of Ag-NP usage and disposal has resulted in increased release into aquatic environments (Benn and Westerhoff 2008;Geranio et al 2009). Recent reports demonstrating that Ag-NPs may be significantly more toxic than their bulk counterpart (Recordati et al 2016;Abramenko et al 2018) highlight the need to investigate their potential ecological impacts.…”

Section: Introductionmentioning

confidence: 99%

Developmental exposure to silver nanoparticles at environmentally relevant concentrations alters swimming behavior in zebrafish (Danio rerio)

González

Carty

Tran

et al. 2018

Enviro Toxic and Chemistry

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Silver nanoparticles (Ag-NP) are ubiquitous in household and medical products because of their antimicrobial activity. A consequence of the high volume of Ag-NP production and usage is increased amounts of Ag-NPs released into the environment. Their small size (between 1–100 nanometers) results in unique physiochemical properties that may increase toxicity relative to their bulk counterpart. Therefore, the goal of this study is to assess the potential toxicity of environmentally relevant concentrations of Ag-NPs in zebrafish (Danio rerio). Wild-type tropical 5D zebrafish embryos were exposed to Ag-NPs from 4 to 120 hours post fertilization (hpf) at 0.03, 0.1, 0.3, 1, 3 ppm (mg/L). Inductively-coupled plasma mass spectrometry (ICP-MS) confirmed concentration-dependent uptake of Ag into zebrafish as well as bioaccumulation over time. A morphological assessment revealed no significant hatching impairment, morphological abnormalities, or mortality at any concentration or time point examined. However, assessment of photomotor behavior at 3 days post fertilization (dpf) revealed significant hyperactivity in the 0.3, 1, and 3 ppm Ag-NP treatment groups. At 4 dpf, significant hyperactivity was observed only in the 3 ppm treatment group, while 5 dpf larvae exposed to Ag-NPs displayed no significant abnormalities in photomotor behavior. These findings suggest that non-teratogenic concentrations of Ag-NPs are capable of causing transient behavioral changes during development.

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Ecotoxicity of different-shaped silver nanoparticles: Case of zebrafish embryos

Cited by 101 publications

References 31 publications

Silver Nanoparticles in Zebrafish (Danio rerio) Embryos: Uptake, Growth and Molecular Responses

Silver Nanoparticles in Zebrafish (Danio rerio) Embryos: Uptake, Growth and Molecular Responses

Chitosan-Stabilized Ag Nanoparticles with Superior Biocompatibility and Their Synergistic Antibacterial Effect in Mixtures with Essential Oils

Developmental exposure to silver nanoparticles at environmentally relevant concentrations alters swimming behavior in zebrafish (Danio rerio)

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