Ecotoxicity and genotoxicity of cadmium in different marine trophic levels

Pavlaki, Maria D.; Araújo, Mário Jorge; Cardoso, Diogo N.; Silva, Ana Rita R.; Cruz, Andreia; Mendo, Sónia; Soares, Amadeu M.V.M.; Calado, Ricardo; Loureiro, Susana

doi:10.1016/j.envpol.2016.05.010

Cited by 73 publications

(38 citation statements)

References 58 publications

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“…Also, the highest concentration of Cd increased the percentage of DNA damage of the control by almost double (1.74 times). It is known that Cd, a well-known carcinogen and teratogen with mutagenic properties, can induce genotoxicity in organisms, including chromosomal aberrations, micronuclei formations, and DNA strand breaks [10,19], with the last characteristics being in accordance with our results. As in the case of dimethoate, Cd contamination could lead to the production of reactive oxygen species, promoting the genotoxic capacity of Cd [11].…”

Section: Comet Assay In Folsomia Candidasupporting

confidence: 91%

The comet assay in Folsomia candida: A suitable approach to assess genotoxicity in collembolans

Cardoso

Silva

Cruz

et al. 2017

Enviro Toxic and Chemistry

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The present study shows the comet assay technique being successfully applied for the first time to one of the most widely used soil organisms in standardized ecotoxicological tests, Folsomia candida, providing a step forward in assessing the genotoxicity induced by xenobiotics. Because collembolans have a high content of chitin, a new methodology was developed in which the heads of the collembolans were separated from the rest of the body, allowing the hemolymph to leak out. This procedure allows the cells to be released, and after lysis the genetic material is available for the comet assay. Among other key procedures, the use of 30 organisms (20- to 22-d-old adults) per replicate and the correct amount of cells with genetic material (translated as 10 μL of suspension) applied on the agarose gel were determinants for the success of the results obtained. The methodology was validated by exposing F. candida to a representative metallic element (cadmium) and a representative of organophosphates, the insecticide dimethoate, for a shorter time period of 10 d, compared with the 28 d for the International Organization for Standardization 11267 method. Within this method, the relatively low percentage of DNA damage (30%) observed in controls and the significant increase in terms of percentage of DNA damage for almost all the concentrations of dimethoate and Cd (reaching 52% and 56% of damage in the highest concentrations, respectively) confirmed the genotoxic effect of both compounds and validated this technique. The comet assay proved to be a sensitive technique to detect DNA strand breaks in collembolans' cells. Environ Toxicol Chem 2017;36:2514-2520. © 2017 SETAC.

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Section: Comet Assay In Folsomia Candidasupporting

confidence: 91%

The comet assay in Folsomia candida: A suitable approach to assess genotoxicity in collembolans

Cardoso

Silva

Cruz

et al. 2017

Enviro Toxic and Chemistry

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“…Recently, increased attention has been focused on studying the adverse effects of toxic commercial products on target and non-target food organisms (Matsumoto et al 2006, Halappa & David 2009, Pavlaki et al 2016, Butcherine et al 2019). These products are often used to control pests in the household or in agricultural fields.…”

Section: Introductionmentioning

confidence: 99%

In vivo DNA damage in gill, haemolymph and muscle cells of whiteleg shrimp Litopenaeus vannamei on exposure to organophosphorus pesticide

Pawar¹,

Sanaye²,

Shyama³

et al. 2019

Aquacult. Environ. Interact.

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In response to growing worldwide market demand, intensive shrimp farming, particularly of whiteleg shrimp Litopenaeus vannamei, has expanded tremendously. The present study investigated induced DNA damage in gill (GL), haemolymph (HL) and muscle (ML) cells in juveniles of L. vannamei (length: 52.2 ± 6.4 mm; weight: 1.78 ± 0.5 g; mean ± SD) exposed to 2 sublethal (SL) concentrations, SL1 (0.36 µg l -1 ) and SL2 (0.18 µg l -1 ), of the organophosphorus pesticide chlorpyrifos (CPF) during 21 d of exposure (DoE). The magnitude of DNA damage (% tail DNA) as measured by the comet assay at specified intervals (3, 7, 14 and 21 DoE) was found to be tissue specific and time and dose dependent (p < 0.05). At the end of the experiment, at 21 DoE, % tail DNA damage was relatively higher at SL1 (53.61 ± 8.71, 49.36 ± 3.42 and 32.40 ± 4.97%) compared to SL2 (39.25 ± 3.90, 32.22 ± 4.21 and 22.66 ± 2.85%) in GL, HL and ML cells, respectively. No significant differences in water quality parameters were found among treated and control aquaria. The significant reduction in specific growth rates (% growth d -1 ) observed in exposed shrimps indicated that SL concentrations of CPF negatively impacted growth in L. vannamei juveniles. A very low 96 h median lethal concentration (1.44 µg l -1 ) indicated sensitivity of L. vannamei juveniles to CPF, suggesting that the species could be used as a bioindicator for assessing pesticide pollution. The study results highlight the implications of extending the farming of L. vannamei to low-salinity inland areas adjacent to traditional agricultural fields.

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“…Copepods provided as diet were previously exposed to 6.88 μg L −1 of cadmium, corresponding to the lowest‐observed‐effective concentration obtained in a previous early–life stage test by Pavlaki et al . Copepods were exposed to cadmium in glass aquariums at a density of 100 copepods L −1 for 48 h and then siphoned with the help of a tube that was covered with a screen at one end, rinsed with ultrapure water to remove excess medium, freeze‐dried for 24 h, and weighed on a microbalance.…”

Section: Methodsmentioning

confidence: 99%

Toxicokinetics of cadmium in Palaemon varians postlarvae under waterborne and/or dietary exposure

Pavlaki

Morgado

Soares

et al. 2018

Enviro Toxic and Chemistry

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The present study assessed cadmium uptake and depuration rates in the euryhaline estuarine shrimp Palaemon varians under different exposure routes. Postlarval shrimp were exposed for 4 d under different exposure routes: contaminated water, contaminated diet, and a 2-way exposure scenario where both contaminated water and diet were used. After exposure, postlarval shrimp were transferred to a clean medium and fed a noncontaminated diet for 96 h. Bioaccumulation via the different exposure routes was modeled with a standard first-order, one-compartment toxicokinetics model and one with an additional parameter reflecting an inert fraction or storage compartment. The simultaneous 2-way exposure (through water and diet) resulted in accumulation being almost twice as high as the sum of the individual exposure routes, thus indicating that accumulation from multiple routes may be more than additive. Cadmium uptake from water was faster than uptake from food maintained for 48 h at that same cadmium concentration. Shrimp were unable to eliminate cadmium from their body, showing no depuration during 96 h after exposure via different routes, thus suggesting that a longer depuration period is needed. Model comparisons did not provide a significantly better fit when the model included the presence of an inert fraction. The present study highlights the importance of assessing accumulation using multiple exposure routes compared with individual routes because the latter may underestimate bioaccumulation. Environ Toxicol Chem 2018;37:1614-1622. © 2018 SETAC.

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Ecotoxicity and genotoxicity of cadmium in different marine trophic levels

Cited by 73 publications

References 58 publications

The comet assay in Folsomia candida: A suitable approach to assess genotoxicity in collembolans

The comet assay in Folsomia candida: A suitable approach to assess genotoxicity in collembolans

In vivo DNA damage in gill, haemolymph and muscle cells of whiteleg shrimp Litopenaeus vannamei on exposure to organophosphorus pesticide

Toxicokinetics of cadmium in Palaemon varians postlarvae under waterborne and/or dietary exposure

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