EcoRI polymorphism of the metastasis-suppressor gene NME1 in Mexican patients with breast cancer

Abstract

Human breast cancer cells with high metastatic potential show reduced expression of the metastasis-suppressor gene NME1. There are two polymorphic sites for the restriction enzymes BglII and EcoRI, both detectable by Southern blot analysis. Although the BglII site has been analyzed for loss of heterozygosity, the biallelic EcoRI site polymorphism has not been studied in association with breast cancer, complications or metastasis. We analyzed EcoRI site allele frequencies in Mexican patients with breast cancer, using polymerase chain reaction -restriction fragment length polymorphisms. The polymorphic allelic frequencies in the cases and reference groups were 0.4215 and 0.3375, respectively; this difference was not statistically significant (chi2=0.8687, p=0.3512). Thus, EcoR1 polymorphic site was not associated with breast cancer in this series, but could be analyzed in association with metastases and might be informative in the evaluation of loss of heterozygosity in women with breast cancer.

Molecular profiling of cervical cancer progression

“…Underexpression of MHC molecules involved in T-lymphocyte-mediated tumour cell recognition may be one important escape mechanism used by metastatic cancer cells, and these cells can be ignored by the immune system for a long time despite the presence of immunocompetent cells (Pantel et al, 1991). NME1, a metastasis-suppressor gene, shows reduced expression in highly metastatic breast cancer cells and was downregulated in cervical cancer LN metastases in this study (Rubio et al, 2006).…”

An integrated analysis of genes and pathways exhibiting metabolic differences between estrogen receptor positive breast cancer cells

“…When compared to the MDA-MB-435 cells, ZR75-1, but not MCF-7 cells, were found to share similarity in the selectivity of genes of electron transport for energy minimization. For genes related to breast cancer estrogen signaling, GSEA analysis showed a progressive down-regulation of keratin 18 and NME1 from luminal A to basal tumors, which also correlated with ER(+)ve MDA-MB-435 cells; low expression of these genes correlates to poor prognosis and metastasis [ 21 , 22 ]. ZR75-1 cells also have a low expression of these genes, indicating a similarity towards the ER(-)ve phenotype.…”

Association of EcoRI polymorphism of the metastasis-suppressor gene NME1 with susceptibility to and severity of non-small cell lung cancer

“…Genetic polymorphism has become useful tools for identifying candidate genes associated with disease. NME1 gene showed a biallelic polymorphism when digested with EcoRI and genomic PCR-SSCP analysis of the metastasis-associated NME1 gene has been studied in breast cancer and colorectal cancer [32,33]. However, the association between NME1 genetic polymorphism and lung cancer susceptibility and disease severity has not been studied before.…”