Ecophysiology and polymorphism of the unicellular extremely natronophilic cyanobacterium Euhalothece sp. Z-M001 from Lake Magadi

Mikhodiuk, O S; Герасименко, Л. М.; Акимов, В. Н.; Ivanovskiĭ, R N; Zavarzin, G. A.

doi:10.1134/s0026261708060106

Cited by 27 publications

(19 citation statements)

References 7 publications

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“…A green layer several mm below the salt crust can be seen in this area of the GSP. All of the cyanobacterial clones were related to unicellular organisms, with the majority being Cyanothece and Euhalothece , both found in solar salterns and hypersaline lakes [21–24]. Related sequences were detected in an earlier cyanobacterial rRNA gene clone library from the GSP and one of the Cyanothece clones was isolated previously [25].…”

Section: Resultsmentioning

confidence: 99%

Culture‐independent analysis of the soil bacterial assemblage at the Great Salt Plains of Oklahoma

Caton¹,

Schneegurt²

2011

J. Basic Microbiol.

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The Great Salt Plains (GSP) of Oklahoma is a natural inland terrestrial hypersaline environment that forms evaporite crusts of mainly NaCl. Previous work described GSP bacterial assemblages through the phylogenetic and phenetic characterization of 105 isolates from 46 phylotypes. The current report describes the same bacterial assemblages through culture-independent 16S rRNA gene clone libraries. Although from similar hypersaline mud flats, the bacterial libraries from two sites, WP3 and WP6, were quite different. The WP3 library was dominated by cyanobacteria, mainly Cyanothece and Euhalothece. The WP6 library was rich in anaerobic sulfur-cycle organisms, including abundant Desulfuromonas. This pattern likely reflects differences in abiotic factors, such as frequency of flooding and hydrologic push. While more than 100 OTUs were identified, the assemblages were not as diverse, based on Shannon indexes, as bacterial communities from oligohaline soils. Since natural inland hypersaline soils are relatively unstudied, it was not clear what kind of bacteria would be present. The bacterial assemblage is predominantly genera typically found in hypersaline systems, although some were relatives of microbes common in oligohaline and marine environments. The bacterial clones did not reflect wide functional diversity, beyond phototrophs, sulfur metabolizers, and numerous heterotrophs.

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Section: Resultsmentioning

confidence: 99%

Culture‐independent analysis of the soil bacterial assemblage at the Great Salt Plains of Oklahoma

Caton¹,

Schneegurt²

2011

J. Basic Microbiol.

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“…B353 22 and Halothece sp. PCC7418 20 , no keto-carotenoid biosynthesis genes such as crtW and crtO were detected. The failure of several Euhalothece crtW/O candidate genes to produce keto-carotenoids in β-Car-accumulating E. coli is consistent with this genomic survey.…”

Section: Salt Acclimation Genes Associated With Ion Homeostasis Compmentioning

confidence: 99%

“…Euhalothece is a single-celled, stenohaline cyanobacterium ( Supplementary Table S1) requiring 7% NaCl for optimal growth. It exhibits considerable morphological variability depending on the pH, and on the concentrations of NaCl and carbonate 20 . Here, we investigated prevailing salt stress resistance strategies adopted by Euhalothece.…”

mentioning

confidence: 99%

Genomic Survey of Salt Acclimation-Related Genes in the Halophilic Cyanobacterium Euhalothece sp. Z-M001

Yang

Song

Cho

et al. 2020

Sci Rep

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, cheol-Ho pan 2 & Youn-il park 1* Like other halophilic cyanobacterial genomes, the de novo-assembled genome of Euhalothece sp. Z-M001 lacks genes encoding keto-carotenoid biosynthesis enzymes, despite the presence of genes encoding carotenoid-binding proteins (cBps). consistent with this, HpLc analysis of carotenoids identified β-carotene and zeaxanthin as the dominant carotenoids. CBPs coexpressed with the zeaxanthin biosynthesis gene increased the survival rates of Escherichia coli strains by preventing antibiotic-induced accumulation of reactive oxygen species (ROS). RNA-seq analysis of Euhalothece revealed that among various salt resistance-related genes, those encoding the na + transporting multiple resistance and pH adaptation (Mrp) systems, glycine betaine biosynthesis enzymes, exopolysaccharide metabolic enzymes, and CBPs were highly upregulated, suggesting their importance in hypersaline habitats. During the early phase of salt deprivation, the amounts of β-carotene and zeaxanthin showed a negative correlation with ROS content. Overall, we propose that in some halophilic cyanobacteria, β-carotene and zeaxanthin, rather than keto-carotenoids, serve as the major chromophores for CBPs, which in turn act as effective antioxidants. Cyanobacteria are photosynthetic prokaryotes that exhibit diverse protective mechanisms to cope with harsh environmental conditions. One of the protective mechanisms involves the use of carotenoids 1 and diverse carotenoid-binding proteins (CBPs) 2 , which play essential roles in protecting the photosynthetic apparatus from damage by both excess light energy and reactive oxygen species (ROS). Most cyanobacterial CBPs bind to β-carotene (β-Car) and its oxygenated derivatives, called xanthophylls (Xan), and are located in photosynthetic protein complexes such as photosystem I (PSI), PSII, and cytochrome b 6 f 3. By contrast, water soluble CBPs bind non-covalently to cyanobacterial keto-carotenoids, such as echinenone (Ech) and canthaxanthin (Can), and to glycosylated-carotenoids such as myxoxanthophyll (Myx) 4 ; CBPs then function as effective energy dissipaters by interacting with the light-harvesting antenna, phycobilisome (PBS) 5. CBPs also bind to zeaxanthin (Zea), albeit with low affinity 6. The orange carotenoid protein (OCP) and its two paralogs, helical carotenoid protein (HCP) and C-terminal domain homolog (CTDH), are the only known soluble CBPs 4. The OCP is composed of two globular domains (one each at the N-and C-terminal ends); a single carotenoid is embedded between the two domains via a hydrophobic interaction 7. Blue light absorption by a keto-carotenoid results in a conformational change in the OCP, from the orange inactive form (OCP°) to the red active form (OCP R). Binding of OCP R to PBS quenches PBS fluorescence, which is prevented by fluorescence recovery protein (FRP) 8. FRP prevents accumulation of the OCP R by interacting with the C-terminal domain of OCP 8. The biological functions of HCPs, which are grouped into nine distinct clades 7 , are largely unknow...

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“…ZM001 was purchased from Culture Collection of Autotrophic Organisms (CCALA), Academy of Sciences of the Czech Republic. The seed was cultured in M-medium, which contains Na 2 CO 3 , 53 g/L; NaHCO 3 , 42 g/L; NaCl, 50 g/L; KCl, 2 g/L; Na 2 SO 4 , 1.4 g/L; KNO 3 , 2.5 g/L; K 2 HPO 4 ·3H 2 O, 0.5 g/L; FeCl 3 , 0.0003 g/L; EDTA, 0.0005 g/L; and 1 mL of the A5 trace element solution (Mikhodyuk et al 2008). The pH of M-medium was 10.5.…”

Section: Strain and Mediummentioning

confidence: 99%

Plastic bag as horizontal photobioreactor on rocking platform driven by water power for culture of alkalihalophilic cyanobacterium

Zhu

Cheng

et al. 2017

Bioresour. Bioprocess.

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Background: Mixing in traditional algae culture system consumes intensive electricity. This should be replaced by nature force to reduce energy cost and, more importantly, to realize positive energy balance of algal biofuel production. This study aims to develop a horizontal photobioreactor, in which mixing can be provided with rocking movement driven by nature force.Results: Simple boxes were used as small-scale horizontal photobioreactors on a rocking platform for culture of alkalihalophilic Euhalothece sp. ZM001. There was no CO 2 gas bubbling since 1.0 M NaHCO 3 supplied sufficient inorganic carbon in it. Effect of culture depth, rocking cycle, and light intensity to algal biomass production, pH change, and DO accumulation were investigated in this system. Biomass concentration of 2.73 g/L was achieved in culture with 2.5 cm depth, and maximum productivity of 17.06 g/m 2 /day was obtained in culture with 10 cm depth. k L a in PBR with different culture depths and rocking cycles was measured, and it was from 0.57 to 33.49 h −1, showing great variation. To test this system at large scale, a plastic bag with a surface area of 1 m 2 was placed on a rocking platform driven by water power, and it resulted in a biomass concentration of 1.88 g/L. Conclusion: These results proved feasibility of a novel photobioreactor system driven by nature force, as well as low cost of manufacturing, and easy scaling-up.

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Ecophysiology and polymorphism of the unicellular extremely natronophilic cyanobacterium Euhalothece sp. Z-M001 from Lake Magadi

Cited by 27 publications

References 7 publications

Culture‐independent analysis of the soil bacterial assemblage at the Great Salt Plains of Oklahoma

Culture‐independent analysis of the soil bacterial assemblage at the Great Salt Plains of Oklahoma

Genomic Survey of Salt Acclimation-Related Genes in the Halophilic Cyanobacterium Euhalothece sp. Z-M001

Plastic bag as horizontal photobioreactor on rocking platform driven by water power for culture of alkalihalophilic cyanobacterium

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