Ecophysiological characterization and toxin profile of two strains of Cylindrospermopsis raciborskii isolated from a subtropical lagoon in Southern Brazil

Miotto, Maria Cecília; Costa, Luíza Dy Fonseca; Brentano, Débora Monteiro; Nader, Camila; Souza, Luana dos Santos de; Gressler, Pablo Diego; Laudares-Silva, Roselane; Yúnes, João; Barufi, José Bonomi; Rörig, Leonardo Rubi

doi:10.1007/s10750-017-3243-y

Cited by 12 publications

(3 citation statements)

References 66 publications

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“…Raphidiopsis raciborskii produces extremely aggressive toxins that can cause both human health problems and damage to the environment. Its high competitiveness in eutrophic environments, combined with its ability to form toxic blooms in nitrogen and phosphorus limited waters (Miotto et al 2017) make this species one of the most studied cyanobacteria from both an ecological and public health point of view. There are two well-known toxins, cylindrospermopsin, an alkaloid with action on the liver and kidneys, and saxitoxin (STX), also known as PSP (Paralytic Shellfish Poisons), which acts on the neuromuscular system (Pratheepa & Vasconcelos 2017, Yang et al 2021.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in silico assessment of cytotoxicity and chromosome instability induced by saxitoxin in human derived neural cell line

Constante

Khateeb

Souza

et al. 2022

An. Acad. Bras. Ciênc.

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In freshwater, saxitoxins (STX) are produced by different cyanobacteria genera, including Raphidiopsis. Data regarding cytogenotoxicity effects of STX on human cells are scarse, this merit further studies of its toxicology. This study assessed the cytotoxicity and the chromosome instability of STX on SHSY-5Y human cell line. The CBMN assay allows the detection of chromosome breaks and abnormal chromosomal segregation. Additionally, in silico systems biology approach, used to search for known and predicted interaction networks, was applied to study the interactions between STX and SHSY-5Y cellular components. The results of the CBMN assay demonstrated that STX concentrations of 2.5 -10 µg/L induced cytostasis and chromosome instability in a dose-response relationship. Apoptosis was detected after exposure of SHSY-5Y cultured cells to STX concentration of 10 µg/L. The results of the systems biology analysis revealed the interaction of STX with proteins related with acetylcoline pathway, cell cycle regulation and apoptosis. Furthermore, combining the in vitro and in silico approachs, it was possible to suggest a mechanism of action of STX in SHSY-5Y cells.Overall, the data demonstrated the cytotoxicity and mutagenicity of environmentally relevant concentrations of STX. These results should be considered when setting up guidelines for monitoring STX in water supply.

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Section: Introductionmentioning

confidence: 99%

In vitro and in silico assessment of cytotoxicity and chromosome instability induced by saxitoxin in human derived neural cell line

Constante

Khateeb

Souza

et al. 2022

An. Acad. Bras. Ciênc.

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“…Substantial ecophysiological differences occur within species (ecotypes; see e.g. Piccini et al, 2011;Miotto et al, 2017), and within species of the same genera (Kust et al, 2015;Lengyel et al, 2020)-all belonging to the same functional group. Minor differences between ecophysiological tolerance levels may basically determine dispersal opportunities (Lengyel et al, 2020) or even competition between species of different functional groups can be strain dependent (Marinho et al, 2013).…”

Section: Food Websmentioning

confidence: 99%

Phytoplankton in extreme environments: importance and consequences of habitat permanency

Padisák

Naselli-Flores

2020

Hydrobiologia

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There is hardly any sunshine exposed surface on this Earth, be it water or terrain, which would not support some biota. Still, many habitats offer harsh conditions requiring specialized physiological adaptations to survive. These environments are referred to as extremes; often inhabited by extremophilic organisms. In this review, characteristic species and assemblage properties of phytoplankton inhabiting extreme environments (especially lakes and pools where planktic life is potentially possible and independently of their origin) in terms of alkalinity, acidity, DOC, salinity, temperature, light and mixing regime will be outlined. Lakes characterized by more than a single extreme are common (e.g. saline + alkaline; acidic + high DOC + high metal content + low light). At the edge of extremes (e.g. pH of 1; salinity over ~ 100–150 g l−1) single species with appropriate physiological adaptation are selected and the phytoplankton is often dominated by a single species (monodominant) setting compositional diversity to zero. Under less extreme conditions permanent equilibria may persist; in many cases over several years in contrast to „average” lakes where equilibria are rare and ephemeral. Food webs depending on „extreme phytoplankton” are often atypical for example because the microbial loop is of prior importance or because birds are top predators.

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“…Although Pi is the preferable source of phosphorous for them, a few studies have shown that Cylindrospermopsis genera can efficiently consume dissolved organic phosphorous, including phosphonates compounds, such as glyphosate and 2-aminoethyl phosphonate, when under Pi scarcity (Bai et al, 2014;Miotto et al, 2017). Willis et al (2018) reported the presence of the complete cluster for the 'C-P lyase' pathway to consume phosphonate in the genomes of Australian strains of C. raciborskii.…”

Section: Discussionmentioning

confidence: 99%

Oxic methane production by cyanobacteria from the Brazilian Pantanal soda lakes

Carvalho¹

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I would like to acknowledge the sponsorship agencies that financially supported the development of my doctorate project. CNPq for my doctoral scholarship (Process N.: 140384/2018-9), CAPES for the PDSE scholarship during my abroad internship (Process N.:88887.588463/2020-00), FEALQ for supporting the last months of my studies (N. 90360), and FAPESP for the thematic project "Climate change and environmental impacts in wetlands (soda lakes) of the Pantanal (Brazil): quantification, control factors, and long-term forecast" (Process N.: 2016/14227-5).With great esteem, I would like to thank my doctoral supervisor Prof. Dr. Marli Fiore for all the support and supervision during these four years of working together. Thank you for having me in your group and for opening a space for me and this unconventional project, it was a time of great learning that will come with me to my next journeys.In tandem, I want to thank the 'Cyanos' group from the Laboratory of Cellular and Molecular Biology. I hope we can meet again in academia and life. Special gratitude to the lab technicians, Dr. Ana Paula Dini Andreote and Me. Renata Beatriz da Cruz for all the help, cues, and advice they kindly shared with us. From the ones who welcomed me,

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Ecophysiological characterization and toxin profile of two strains of Cylindrospermopsis raciborskii isolated from a subtropical lagoon in Southern Brazil

Cited by 12 publications

References 66 publications

In vitro and in silico assessment of cytotoxicity and chromosome instability induced by saxitoxin in human derived neural cell line

In vitro and in silico assessment of cytotoxicity and chromosome instability induced by saxitoxin in human derived neural cell line

Phytoplankton in extreme environments: importance and consequences of habitat permanency

Oxic methane production by cyanobacteria from the Brazilian Pantanal soda lakes

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