Economizing the lignocellulosic hydrolysis process using heterologously expressed auxiliary enzymes feruloyl esterase D (CE1) and β-xylosidase (GH43) derived from thermophilic fungi Scytalidium thermophilum

Agrawal, Dhruv; Tsang, Adrian; Chadha, Bhupinder Singh

doi:10.1016/j.biortech.2021.125603

Cited by 22 publications

(4 citation statements)

References 44 publications

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“…However, Co + , Zn 2+ , Fe 3+ , and Mg 2+ inhibited the enzyme activ about 20%, while Na + had a slight promotion effect on the enzyme activity. Different previous studies, Agrawal et al found that Mg 2+ , Ca + , and Cu 2+ promoted FAE de from Pichia pastoris X33, while Na + inhibited FAE [26]. This reflects that sodium ions different roles on FAEs from different sources-some inhibit and some promote, refle the species diversity of FAEs.…”

Section: Effect Of Metal Ions On Kofae Activitymentioning

confidence: 83%

Characterization of Feruloyl Esterase from Klebsiella oxytoca Z28 and Its Application in the Release of Ferulic Acid from De-Starching Wheat Bran

et al. 2023

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Feruloyl esterase (EC3.1.1.73; FAE) can degrade biomass to release ferulic acid (FA), which has a high application in bioprocessing, food, pharmaceutical, paper, feed, and other industrial fields. A strain of Klebsiella oxytoca Z28 with ferulic esterase activity was screened from Daqu. In addition, the FAE gene was expressed in Escherichia coli BL21 (DE3). The enzyme consists of 340 amino acids with a molecular mass of 37.7 kDa. The FAE enzyme activity was 463 U/L when the substrate was ethyl 4-hydroxy-3-methoxycinnamate and the optimum temperature and pH were 50 °C and 8.0, respectively. The enzyme had good stability at temperatures of 25–40 °C and a pH of 8.0. Ba2+, Cu2+, Mn2+, and Ca2+ had a strong inhibitory effect on the enzyme activity, and Na+ had a promotive effect on the enzyme activity. The de-starching wheat bran was degraded by KoFAE, and the FA release was up to 227.15 µg/g. This indicated that the heterologous expression of KoFAE from Klebsiella oxytoca Z28 in E. coli had a certain potential of biodegradation, which can be applied to the degradation of agricultural waste to obtain high value-added FA products.

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Section: Effect Of Metal Ions On Kofae Activitymentioning

confidence: 83%

Characterization of Feruloyl Esterase from Klebsiella oxytoca Z28 and Its Application in the Release of Ferulic Acid from De-Starching Wheat Bran

et al. 2023

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“…The CE class was investigated next, and it was found to be functionally enriched for hemicellulytic activity (Figure 6b). The most prominent CE family identified was CE1, which contains acetyl xylan esterases and feruloyl esterases and has been utilized as components in the biotechnological applications for the degradation of lignocellulytic biomass (Agrawal et al., 2021; Dilokpimol et al., 2022; Goldbeck et al., 2016; Mäkelä et al., 2018). Subsequently, the CE12 family also contains acetyl xylan esterases, as well as pectin acetylesterases and rhamnogalacturonan acetylesterase, which have been associated with the degradation of pectin in addition to hemicellulose (Bonnin et al., 2008; Razeq et al., 2018).…”

Section: Discussionmentioning

confidence: 99%

A metagenomic survey of the fecal microbiome of the African savanna elephant (Loxodonta africana)

du Preez,

van der Walt,

Valverde

et al. 2024

Animal Genetics

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The African savanna elephant (Loxodonta africana) is the largest terrestrial animal on Earth and is found primarily in Southern and Eastern Africa. It is a hindgut, colonic fermenter and subsists on a diet of raw plant materials found in its grazing area. In this study the bacterial, archaeal and fungal populations of seven African savanna elephant fecal metagenomes were first characterized using amplicon sequencing. On the genus level it was observed that the p‐1088‐a5 gut group in the bacteriome, Methanocorpusulum and Methanobrevibacter in the archaeome and Alternaria, Aurobasidium, Didymella and Preussia in the mycome, predominated. Subsequently, metagenomic shotgun sequencing was employed to identify possible functional pathways and carbohydrate‐active enzymes (CAZymes). Carbohydrate catabolic pathways represented the main degradation pathways, and the fecal metagenome was enriched in the glycohydroside (GH) class of CAZymes. Additionally, the top GH families identified – GH43, GH2, GH13 and GH3 – are known to be associated with cellulytic, hemicellulytic and pectolytic activities. Finally, the CAZymes families identified in the African savanna elephant were compared with those found in the Asian elephant and it was demonstrated that there is a unique repository of CAZymes that could be leveraged in the biotechnological context such as the degradation of lignocellulose for the production of second‐generation biofuels and energy.

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“…Among the hemicellulases, xylanases act in the depolymerization of hemicelluloses and help in the conversion of biomass, contributing to the opening of the fibers and to the access of cellulases to cellulose (Amorim et al, 2019). Agrawal et al (2021), for example, produced D-feruloyl esterase and β-xylosidase by the fungus Scytalidium thermophilum and observed that the use of these homemade cocktails improved the hydrolysis of rice straw and sugarcane bagasse, demonstrating potential to reduce the load of commercial enzymes, and consequently, the cost of the sugars production from agricultural wastes. The action of accessory enzymes was also evaluated by Nwamba et al (2021), who employed β-glucosidase (Novozymes) and endoxylanase (Qingdao Vland Biotech) in association with cellulolytic cocktail LT4, in the hydrolysis of sugarcane bagasse and released high concentration of glucose (131 g/L).…”

Section: Influence Of the Enzymatic Synergismmentioning

confidence: 99%

Factors that influence the enzymatic hydrolysis of agricultural wastes for ethanol production: a review

Santos

Pasquini²,

Baffi³

2022

J. Eng. Exact Sci.

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Lignocellulosic biomass, such as agricultural and forestry residues, can be reused and serve as sources of sugars for the production of second-generation ethanol (2G) and other bioproducts. However, these wastes are composed by molecules of difficult degradation, which require steps of pretreatment and enzymatic hydrolysis for their bioconversion into fermentable sugars. At the same time, chemical substances with a potential inhibitory effect on the microbial metabolism can also be produced after the pretreatments and hinder the overall yield of the hydrolytic process. For an efficient and low-cost hydrolysis, homemade enzymes produced from agroindustrial residues, such as sugarcane bagasse, can be employed. However, a set of parameters might be adjusted, such as: kind of pretreatment, enzyme load, solids load, hydrolysis time and use of additives, to improve the yields in free sugars using these onsite enzymatic preparations. In this sense, studies involving the optimization of the conditions of pretreatment and saccharification are essential to increase the bioconversion rate of lignocellulose. These strategies are important for the production of value-added products from these wastes and, consequently, offer a correct and profitable destination to them. Hence, this study presents a review of the main features that influence the enzymatic hydrolysis of agricultural wastes and the yield in reducing sugars for ethanol production.

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Economizing the lignocellulosic hydrolysis process using heterologously expressed auxiliary enzymes feruloyl esterase D (CE1) and β-xylosidase (GH43) derived from thermophilic fungi Scytalidium thermophilum

Cited by 22 publications

References 44 publications

Characterization of Feruloyl Esterase from Klebsiella oxytoca Z28 and Its Application in the Release of Ferulic Acid from De-Starching Wheat Bran

Characterization of Feruloyl Esterase from Klebsiella oxytoca Z28 and Its Application in the Release of Ferulic Acid from De-Starching Wheat Bran

A metagenomic survey of the fecal microbiome of the African savanna elephant (Loxodonta africana)

Factors that influence the enzymatic hydrolysis of agricultural wastes for ethanol production: a review

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