Economic Analysis of Efficiency of RB51 Strain Vaccine of Brucella abortus Applied in Herds Naturally Infected with Brucellosis in Tropical Climate

Martinez-H, David; Peniche-Ca, Alvaro; Franco-Zam, Jose Luis; Canudas-La, Eduardo; Barradas-P, Francisco; Molina-San, Baldomero; Gutierrez, Edwin Jose; Williams, José de Jesús; Morales-Al, Jose Francisco; Flores-Cas, Ricardo

doi:10.3923/javaa.2012.1784.1789

Cited by 1 publication

(2 citation statements)

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“…abortus is the main etiological agent of brucellosis, a zoonotic disease that affects numerous animal species, causing serious economic loss and posing a public health threat (2)(3)(4)(5)(6)(7)27). Therefore, many research efforts have focused on characterizing new virulence factors of B. abortus with the aim of developing live vaccine strains that combine both safety and efficacy (11).…”

Section: Discussionmentioning

confidence: 99%

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Mutant Brucella abortus Membrane Fusogenic Protein Induces Protection against Challenge Infection in Mice

et al. 2015

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f Brucella species can cause brucellosis, a zoonotic disease that causes serious livestock economic losses and represents a public health threat. The mechanism of virulence of Brucella spp. is not yet fully understood. Therefore, it is crucial to identify new molecules that serve as virulence factors to better understand this host-pathogen interplay. Here, we evaluated the role of the Brucella membrane fusogenic protein (Mfp) and outer membrane protein 19 (Omp19) in bacterial pathogenesis. In this study, we showed that B. abortus ⌬mfp::kan and ⌬omp19::kan deletion mutant strains have reduced persistence in vivo in C57BL/6 and interferon regulatory factor 1 (IRF-1) knockout (KO) mice. Additionally, 24 h after macrophage infection with a ⌬mfp::kan or ⌬omp19::kan strain expressing green fluorescent protein (GFP) approximately 80% or 65% of Brucella-containing vacuoles (BCVs) retained the late endosomal/lysosomal marker LAMP-1, respectively, whereas around 60% of BCVs containing wild-type S2308 were found in LAMP-1-negative compartments. B. abortus ⌬omp19::kan was attenuated in vivo but had a residual virulence in C57BL/6 and IRF-1 KO mice, whereas the ⌬mfp::kan strain had a lower virulence in these same mouse models. Furthermore, ⌬mfp::kan and ⌬omp19::kan strains were used as live vaccines. Challenge experiments revealed that in C57BL/6 and IRF-1 KO mice, the ⌬mfp::kan strain induced greater protection than the vaccine RB51 and protection similar that of vaccine S19. However, a ⌬omp19::kan strain induced protection similar to that of RB51. Thus, these results demonstrate that Brucella Mfp and Omp19 are critical for full bacterial virulence and that the ⌬mfp::kan mutant may serve as a potential vaccine candidate in future studies. Brucellosis is a chronic infectious disease caused by bacteria of the genus Brucella. This disease affects many species of animals, resulting in great economic losses, and is therefore an important bacterial zoonotic disease worldwide (1). The genus Brucella replicates inside trophoblasts, macrophages, and dendritic cells and colonizes the reticuloendothelial system and reproductive organs (2). Additionally, brucellosis is not only the major cause of abortion and infertility in animals but also a debilitating disease in humans (2-7).To overcome the immune system and establish a chronic infection, B. abortus utilizes diverse evasion mechanisms. This pathogen can penetrate host cells through lipid rafts (8). Once inside cells, the establishment of a persistent infection relies on the ability of the bacterium to form a Brucella-containing vacuole (BCV), which traffics from the endocytic compartment to the endoplasmic reticulum (ER), forming a replicative BCV. It is in this replicative BCV that the bacteria begin to multiply (8, 9).Extensive vaccination programs have been undertaken to prevent brucellosis in animals. Despite their availability, live vaccine strains have critical disadvantages (10). The main vaccines currently available for brucellosis are S19 and RB51 (derived from B. abortus)...

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Section: Discussionmentioning

confidence: 99%

“…The genus Brucella replicates inside trophoblasts, macrophages, and dendritic cells and colonizes the reticuloendothelial system and reproductive organs (2). Additionally, brucellosis is not only the major cause of abortion and infertility in animals but also a debilitating disease in humans (2)(3)(4)(5)(6)(7).…”

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confidence: 99%