Ecological Studies on the Lactobacillus Flora Associated with the Crop Epithelium of the Fowl

Fuller, R.

doi:10.1111/j.1365-2672.1973.tb04080.x

Cited by 177 publications

(89 citation statements)

References 12 publications

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“…In a high proportion of the chickens examined lactobacilli appeared to colonise the epithelium of the crop in preference to the lumen. The extensive colonisation of this organ by lactobacilli has been confirmed by microscopy (Fuller, 1973) and serves as a useful control with which to compare E. coli and Salmonella. E. coli were found to associate preferentially with the crop epithelium but in fewer chickens.…”

Section: Discussionmentioning

confidence: 99%

“…The isolation of an equal number of bacteria from an epithelium than from the associated luminal contents was the criterion used by Smith and Halls, (1968) and modified by Fuller (1973) to indicate a physical association between the bacteria and the epithelium. The number of occasions on which the viable bacterial counts obtained from the epithelia of the crop and caeca were higher than the counts from the contents of these organs for all the bacterial strains studied is shown in Table 3.…”

Section: In Vivo Association Of Salmonella Strains With Crop and Caecmentioning

confidence: 99%

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Intestinal colonisation in the chicken by food‐poisoningsalmonellaserotypes; Microbial characteristics associated with faecal excretion

Barrow¹,

Simpson²,

Lovell³

1988

Avian Pathology

174

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SUMMARYFollowing oral inoculation of newly-hatched or three-week-old chickens, Salmonella organisms persisted longest in the caeca and they were also present for a shorter time in the crop. S. cholerae-suis, E. coli K12 and Saccharomyces cerevisiae did not colonise the alimentary tract when inoculated orally, but they persisted longer in the caeca than in other regions of the gut. This suggested that preferential localisation of Salmonella in the caeca may be the result of non-specific or host factors. This may include the slow rate of flow of contents through this organ which would allow greater microbial multiplication and persistence. Some Salmonella strains, particularly S. typhimurium, inoculated orally into three-week-old chickens were isolated in greater numbers from homogenates of the crop wall and, much less frequently, from the caecal wall, than from the luminal contents of these organs. None of the strains associated with the crop wall to the same extent as did lactobacilli.After oral inoculation of food-poisoning Salmonella serotypes the number of chickens excreting Salmonellae in their faeces gradually declined over a period of at least 4 weeks. In contrast, host adapted serotypes such as S. cholerae-suis and S. abortus-ovis were not excreted for longer than a few days. By testing mutants of S. typhimurium and S. infantis which were devoid of easily recognised characteristics it was concluded that neither flagellar (H) antigens, somatic (O) antigens, mannose-sensitive haemagglutinins nor the possession of the virulenceassociated 85 kilobase plasmid of S. typhimurium, were essential for colonisation (persistent faecal excretion). However, a mutant of S. infantis which was created by nitroso-guanidine treatment still possessed flagellar and somatic antigens and haemagglutinins and yet did not colonise the alimentary tract, indicating that an unidentified microbial factor was essential for colonisation.

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Section: Discussionmentioning

confidence: 99%

Section: In Vivo Association Of Salmonella Strains With Crop and Caecmentioning

confidence: 99%

Intestinal colonisation in the chicken by food‐poisoningsalmonellaserotypes; Microbial characteristics associated with faecal excretion

Barrow¹,

Simpson²,

Lovell³

1988

Avian Pathology

174

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“…This is consistent with other studies which demonstrate the host specificity of adhesion of Lactobacillus spp. to chicken and rodent gastric epithelia (Fuller, 1973;Suegara t't al., 1975;Lin & Savage, 1984). Because adhesion of bacteria to mouse stomach epithelium was enhanced proportionally to the amount of the adhesion-promoting material added, it may be that adhesion is mediated by multiple binding sites on the bacterial cell surface.…”

Section: P L Conway a N D S K J E L L E B E R Gmentioning

confidence: 99%

“…Host specificity in the colonization of the gastrointestinal tract by lactobacilli has been demonstrated both in uivo and in vitro for a range of hosts (Fuller, 1973;Suegara et al, 1975;Barrow et al, 1980;Tannock et al, 1982;Lin & Savage, 1984). Savage (1972) proposed that adhesion of indigenous Lactobacillus spp.…”

Section: Introductionmentioning

confidence: 99%

Protein-mediated Adhesion of Lactobacillus fermentum Strain 737 to Mouse Stomach Squamous Epithelium

Conway

Kjelleberg

1989

Microbiology

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The mechanism of adhesion of Lactobacillus fermentum strain 737 to mouse stomach squamous epithelium was investigated. Adhesion inhibition tests involving chelators, monosaccharides, periodate and concanavalin A and the use of bacteria grown in the presence of tunicamycin failed to clarify the adhesive mechanism. Washed bacterial cells had reduced adhesive capacity, except in the presence of spent broth culture supernatant fraction or cell washings. Spent culture supernatant fractions of erythrosine-supplemented broth did not enhance adhesion of washed cells. The adhesion-promoting factor(s) in the spent broth culture supernatant fractions and cell washings bound to both bacterial and epithelial cell surfaces, but did not promote adhesion of two other Lactobacillus strains which were not of mouse origin, thereby indicating host specificity for the adhesion-promoting activity. Chemical characteristics of the adhesionpromoting factor were determined by pretreatment of the dialysis retentate of spent broth culture supernatant fractions with proteolytic enzymes, concanavalin A-Sepharose or periodate before the adhesion assay. The adhesin was non-dialysable, pronase-sensitive, heat sensitive at 100 "C, had no affinity for concanavalin A-Sepharose and contained no carbohydrate groups active in the adhesion process. The protein profiles of dialysis retentates of spent broth culture supernatant fractions after bacterial growth in the absence and presence of erythrosine were determined by 2-dimensional SDS-PAGE. Gel filtration by HPLC was used for purification of an adhesion-promoting fraction. The host-specific adhesion of L. fermenturn strain 737 was mediated by a protein, with an M , of 12-13000, that was not detectable in cells grown in the presence of erythrosine. A model for the mode of binding of the adhesin to host epithelia and bacterial surfaces is proposed.

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“…For the administration of Lactobacillus culture to animals to be of practical use, the following conditions are essential: i) host specificity of the microorganism used (4,19), and ii) their ability to form stable populations in the gastrointestinal tract (20).…”

mentioning

confidence: 99%

Colonization of the gastrointestinal tracts of conventional piglets by Lactobacillus strains.

Sarra

Cantalupo

Massa

et al. 1991

J. Gen. Appl. Microbiol.

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Ecological Studies on the Lactobacillus Flora Associated with the Crop Epithelium of the Fowl

Cited by 177 publications

References 12 publications

Intestinal colonisation in the chicken by food‐poisoningsalmonellaserotypes; Microbial characteristics associated with faecal excretion

Intestinal colonisation in the chicken by food‐poisoningsalmonellaserotypes; Microbial characteristics associated with faecal excretion

Protein-mediated Adhesion of Lactobacillus fermentum Strain 737 to Mouse Stomach Squamous Epithelium

Colonization of the gastrointestinal tracts of conventional piglets by Lactobacillus strains.

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