Ecological strategies of biological and chemical control agents on wildfire disease of tobacco (Nicotiana tabacum L.)

Liu, Tianbo; Gu, Yanling; Zhou, Zhi; Liu, Zhenghua; Yin, Huaqun; Qin, Chong; Yi, Tuyong; Tao, Jiemeng

doi:10.1186/s12866-021-02237-8

Cited by 14 publications

(11 citation statements)

References 55 publications

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“…These results are consistent with other studies indicating that different application frequencies of mancozeb did not signi cantly alter the richness and diversity of rhizosphere bacterial community of the soil (Huang et al, 2021c;You et al, 2016). Furthermore, previous studies indicate that biocontrol agents help increase soil bacterial diversity and strengthen homogenous bacterial communities rather than fungicide treatment (You et al, 2016;Liu et al, 2021b).…”

Section: Discussionsupporting

confidence: 92%

Response of microbial communities on tobacco leaf phyllosphere exposed to the broad-spectrum fungicide mancozeb

Dai

Wang³

et al. 2023

Preprint

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Mancozeb is a broad-spectrum fungicide frequently applied as foliar spray in tobacco fields to control fungal diseases. The response of tobacco phyllosphere microbiota toward mancozeb stress was assessed using high-throughput sequencing at four time points: before spraying, and 5, 10 and 15 days after fungicide application. Results showed that the foliar application of mancozeb had moderate but significant effect on fungal community composition of tobacco phyllosphere. In all samples, Ascomycota and Proteobacteria were the most abundant phyla, and Alternaria was the dominant fungal genus. Moreover, mancozeb significantly affected indigenous bacterial communities of tobacco leaves; Pseudomonas was predominant in untreated and before mancozeb treatment groups. An increase in abundance of Ascomycota was observed in diseased samples while healthy samples showed a reduction after mancozeb application. Increased abundance of Proteobacteria was observed in treated samples, which was much higher in diseased than in healthy groups. Increased abundance of Alternaria was observed until 15 days after mancozeb application, while successive reduction in abundance of the genus was observed in the untreated healthy group. Fungal alpha diversity indices in untreated groups increased from the first collection stage to the third, whereas a decrease in four fungal indices was observed at 5 d after mancozeb exposure. A significant difference between treated and untreated groups was observed in terms of fungal richness indices of similar samples from the previous stage. Bacterial diversity indices increased after mancozeb application while they decreased in untreated samples. Mancozeb was effective in altering the fungal community structure rather than bacterial community structure. After mancozeb treatment, the proportion of pathotroph-saprotroph-symbiotroph increased while it decreased in the untreated groups. The overall findings revealed ecological implications of the effects of mancozeb on tobacco phyllosphere microbiome; our results would provide a theoretical basis for future studies on microecological protection of phyllosphere.

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Section: Discussionsupporting

confidence: 92%

Response of microbial communities on tobacco leaf phyllosphere exposed to the broad-spectrum fungicide mancozeb

Dai

Wang³

et al. 2023

Preprint

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“…Other frequently present keystone taxa includes Sphingomonas and Labrys. These taxa are reported to be dominant in plant phyllosphere and might play important roles in inhibiting plant disease ( Liu et al., 2021 ; Liu et al., 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Responses of the bacterial community of tobacco phyllosphere to summer climate and wildfire disease

Wang

Tian

et al. 2022

Front. Plant Sci.

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Both biotic and abiotic factors continually affect the phyllospheric ecology of plants. A better understanding of the drivers of phyllospheric community structure and multitrophic interactions is vital for developing plant protection strategies. In this study, 16S rRNA high-throughput sequencing was applied to study how summer climatic factors and bacterial wildfire disease have affected the composition and assembly of the bacterial community of tobacco (Nicotiana tabacum L.) phyllosphere. Our results indicated that three time series groups (T1, T2 and T3) formed significantly distinct clusters. The neutral community model (NCM) and beta nearest taxon index (betaNTI) demonstrated that the overall bacterial community assembly was predominantly driven by stochastic processes. Variance partitioning analysis (VPA) further showed that the complete set of the morbidity and climatic variables together could explain 35.7% of the variation of bacterial communities. The node numbers of the molecular ecological networks (MENs) showed an overall uptrend from T1 to T3. Besides, Pseudomonas is the keystone taxa in the MENs from T1 to T3. PICRUSt2 predictions revealed significantly more abundant genes of osmoprotectant biosynthesis/transport in T2, and more genes for pathogenicity and metabolizing organic substrate in T3. Together, this study provides insights into spatiotemporal patterns, processes and response mechanisms underlying the phyllospheric bacterial community.

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“…Previous studies show contradictory results of either increment or reduction in the abundance of fungal community and diversity of tobacco leaves infected by Golovinomyces cichoracearum, Didymella segeticola , and A. alternata (Guo et al, 2020 ; Huang et al, 2020 ; Xiang et al, 2020 ). Moreover, pesticide treatments have been documented to influence bacterial communities colonizing the tobacco phyllosphere (Chen X. et al, 2021 ; Liu et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Spatio-Temporal Variation in the Phyllospheric Microbial Biodiversity of Alternaria Alternata-Infected Tobacco Foliage

Dai

Wang³

et al. 2022

Front. Microbiol.

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Phyllospheric microbial composition of tobacco (Nicotiana tabacum L.) is contingent upon certain factors, such as the growth stage of the plant, leaf position, and cultivar and its geographical location, which influence, either directly or indirectly, the growth, overall health, and production of the tobacco plant. To better understand the spatiotemporal variation of the community and the divergence of phyllospheric microflora, procured from healthy and diseased tobacco leaves infected by Alternaria alternata, the current study employed microbe culturing, high-throughput technique, and BIOLOG ECO. Microbe culturing resulted in the isolation of 153 culturable fungal isolates belonging to 33 genera and 99 bacterial isolates belonging to 15 genera. High-throughput sequencing revealed that the phyllosphere of tobacco was dominantly colonized by Ascomycota and Proteobacteria, whereas, the most abundant fungal and bacterial genera were Alternaria and Pseudomonas. The relative abundance of Alternaria increased in the upper and middle healthy groups from the first collection time to the third, whereas, the relative abundance of Pseudomonas, Sphingomonas, and Methylobacterium from the same positions increased during gradual leaf aging. Non-metric multi-dimensional scaling (NMDs) showed clustering of fungal communities in healthy samples, while bacterial communities of all diseased and healthy groups were found scattered. FUNGuild analysis, from the first collection stage to the third one in both groups, indicated an increase in the relative abundance of Pathotroph-Saprotroph, Pathotroph-Saprotroph-Symbiotroph, and Pathotroph-Symbiotroph. Inclusive of all samples, as per the PICRUSt analysis, the predominant pathway was metabolism function accounting for 50.03%. The average values of omnilog units (OUs) showed relatively higher utilization rates of carbon sources by the microbial flora of healthy leaves. According to the analysis of genus abundances, leaf growth and leaf position were the important drivers of change in structuring the microbial communities. The current findings revealed the complex ecological dynamics that occur in the phyllospheric microbial communities over the course of a spatiotemporal varying environment with the development of tobacco brown spots, highlighting the importance of community succession.

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Ecological strategies of biological and chemical control agents on wildfire disease of tobacco (Nicotiana tabacum L.)

Cited by 14 publications

References 55 publications

Response of microbial communities on tobacco leaf phyllosphere exposed to the broad-spectrum fungicide mancozeb

Response of microbial communities on tobacco leaf phyllosphere exposed to the broad-spectrum fungicide mancozeb

Responses of the bacterial community of tobacco phyllosphere to summer climate and wildfire disease

Spatio-Temporal Variation in the Phyllospheric Microbial Biodiversity of Alternaria Alternata-Infected Tobacco Foliage

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