Ecological Factors of Hydrolytic Enzyme Activities in the Ob–Yenisey Estuarine Coastal Waters in Winter and Their Modelling

Bardan, S. I.; Корнеева, Г. А.

doi:10.1023/b:bibu.0000043779.46983.78

Cited by 6 publications

(5 citation statements)

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“…Conversely, higher levels of proteolytic activity closer to the river's headwaters suggest that proteic substrates were abundant at that site. Similarly, Bardan and Korneeva (2004) found higher proteolytic activity in estuarine waters and decreased activity levels seaward. Moreover, the Alcantara River had higher LAP and GLU activities compared to the more marine sites because of relatively higher bacterioplankton biomass in the river than in the sea, as also observed by Saliot et al (1996) in the Lena River delta and Laptev Sea.…”

Section: Discussionmentioning

confidence: 81%

Multidisciplinary ecological assessment of the Alcantara River (Sicily, Italy) using bioindicators

Guglielmo

Azzaro

Baviera

et al. 2014

Mar. Freshwater Res.

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This study was developed within the framework of a broad international project, 'Ecological water quality assessment of the Alcantara (Italy), James (USA) and Guadalfeo (Spain) rivers using bioindicators', established by the Center for Integrative Mediterranean Studies (CIMS), a collaborative research centre consisting of the University of Messina -Italy, Virginia Commonwealth University -USA, and the University of Cordoba -Spain. The primary objective of the study was the validation of a multi-disciplinary ecological approach at different taxonomic levels for biomonitoring of the Alcantara River (Sicily, Italy) using bioindicators. This study examined the primary physical, chemical and biological features of the river through an interdisciplinary and synoptic approach using bioindicators that included riparian plant physiology, the microbial, zooplanktonic and macroinvertebrate assemblages, Coleoptera (Insecta) and river hydrology. Sampling of the river and riparian areas was conducted at sites from the river's headwaters to the mouth. The study provided information on the ecological status of the Alcantara River along its course and tested the use of a variety of bioindicators, rather than a single biotic or physicalattribute, to determine the river's health.

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Section: Discussionmentioning

confidence: 81%

Multidisciplinary ecological assessment of the Alcantara River (Sicily, Italy) using bioindicators

Guglielmo

Azzaro

Baviera

et al. 2014

Mar. Freshwater Res.

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“…Enzyme analysis of sea water included detection of proteinase and amylase activities, calculation of the rates of biopolymer (azocasein and starch) degradation in seawater, and ecological assessment of the environment using highly sensitive standard enzyme-substrate tests (trypsin-azocasein and α -amylase-Procion-5CX-modified starch) detecting natural and anthropogenic inhibitors of biochemical destruction of organic compounds in the environment (Korneeva et al ., 1993b;Korneeva, 2000;Bardan and Korneeva, 2004). The quality of water was expressed as substrate turnover period ( t , h) in the presence of the corresponding enzyme using the conventional water quality standards (Korneeva, 2000;Bardan and Korneeva, 2004) ( Table 1).…”

Section: Methodsmentioning

confidence: 99%

“…Previously hydrolytic enzyme activities were detected in all components of marine ecosystems-water, bottom sediments, snow, ice, and marine suspension (Lochte and Turlay, 1988;Henrich et al ., 1989;Meinecke and Wefer, 1990;Smith et al ., 1992;Korneeva et al ., 1993aKorneeva et al ., , 1993bKorneeva et al ., , 1995Korneeva et al ., , 1996Korneeva et al ., , 2002Boetius and Lochte, 1994;Vetter and Deming, 1994;Bodungen et al ., 1995;Turley et al ., 1995;Borsheim and Myklestad, 1997;Lochte et al ., 1998;Vasil'chuk et al ., 1998;Korneeva and Luneva, 1999;Korneeva, 2000;Danovaro et al ., 2001;Misic et al ., 2002;Mel'nikov et al ., 2003;Korneeva and Gordeeva, 2004;Bardan and Korneeva, 2004;Shevchenko et al ., 2004). The development of enzyme techniques in oceanography opens broad opportunities to study the functioning and interactions between the components and factors of complex marine ecosystems.…”

Section: Variation In Ecological Status Of Norwegian Sea Watermentioning

confidence: 99%

“…In recent 15 years, integrated RV expeditions to the Arctic Regions by the Shirshov Institute of Oceanology of the Russian Academy of Sciences, including us, studied water bodies, snow, ice, and bottom sediment columns using original enzyme express tests for biochemical destruction of high molecular weight organic compounds, proteins and polysaccharides, and conducted ecological assessment of the area using enzyme-substrate tests (Korneeva et al ., 1993a(Korneeva et al ., , b, 1995(Korneeva et al ., , 2002Korneeva, 1996Korneeva, , 2000Vedernikov et al ., 1996aVedernikov et al ., , 1996bShevchenko et al ., 1997Shevchenko et al ., , 2004Vasil'chuk et al ., 1998;Korneeva and Luneva, 1999;Koukina et al ., 2003;Mel'nikov et al ., 2003;Bardan and Korneeva, 2004;Korneeva and Gordeeva, 2004). Studies were carried out in the Barents Sea (9th cruise of RV Professor Logachev, August 1993; coastal expedition to the biological station in Dal'nie Zelentsy of the Murmansk Marine Biological Institute of the Russian Academy of Sciences, 1995; and 11th cruise of RV Akademik Sergey Vavilov, 1997).…”

mentioning

confidence: 99%

“…Studies were carried out in the Barents Sea (9th cruise of RV Professor Logachev, August 1993; coastal expedition to the biological station in Dal'nie Zelentsy of the Murmansk Marine Biological Institute of the Russian Academy of Sciences, 1995; and 11th cruise of RV Akademik Sergey Vavilov, 1997). A broad range of ecological and biochemical tests were performed in the Pechora and Kara Seas by the Murmansk Marine Biological Institute of the Russian Academy of Sciences (nuclear icebreaker Yamal, winter 2002(nuclear icebreaker Yamal, winter -2003 (Bardan and Korneeva, 2004). Marine and coastal expeditions were almost annually conducted to the White Sea in different seasons of 1992-2004(Korneeva and Luneva, 1999Koukina et al ., 2003;Mel'nikov et al ., 2003;Korneeva and Khusid et al ., 2004).…”

mentioning

confidence: 99%

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Variation in Ecological Status of Norwegian Sea Water Determined from Hydrolytic Enzyme Activities

Корнеева

Gordeeva²,

Шевченко

2005

Biol Bull Russ Acad Sci

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The capacity for biopolymer transformation involving efficient and highly specific natural enzyme mechanisms was studied in seawater of the dynamic zone of the Norwegian Sea (the Voring Plateau region). Vertical and spatial variation in proteinase and amylase activities was demonstrated in seawater and the potential rates of degradation of specific substrates, azocasein and Procion-5CX-modified starch, were calculated. High proteolytic activity was demonstrated for the upper photic layer (0-10 m) in the southwestern part of the polygon (up to 88 U/l; v pr = 7.04 mg/l/h). Proteolytic activity in the bathyal layer (1500 m and below) sharply decreased to 8-16 U/l; v pr = 0.64-1.28 mg/l/h. Similar to other regions of the ocean, the pattern of amylase activity in seawater included low rates of polysaccharide destruction (0-4 U/l; v st = 0-0.2 mg/l/h) in water with high proteolytic capacity and, conversely, the top amylase activity (up to 246-490 U/l; v st 12.3-24.5 mg/l/h) in seawater layers with undetectable or low proteolytic activity. The spatial distribution of the enzyme activities can indicate the presence of waters of different origin. In the southwestern part of the polygon, statistical analysis demonstrated high correlations between hydrophysical indices (temperature, salinity, and salinity gradient) and proteinase and amylase activities. The ecological evaluation based on express enzyme-substrate tests demonstrated a stressful situation for destruction of proteins in both the photic layer and the layers below 1000 m ( t pr ≥ 10 h). ECOLOGY

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Functioning of the Pelagic Ecosystems of Aquatic Areas Covered with Ice in the Barents and Kara Seas in Winter and Spring

et al. 2005

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The Murmansk Institute of Marine Biology has been performing sea expeditions on board nuclearpowered icebreakers of the Murmansk Marine Shipping Company beginning from February 1996 (Fig. 1). The annual studies consist of the year-round ecosystem monitoring of Arctic seas, including the hydrological, hydrochemical, microbiological, and algal/planktonic survey; netting zooplankton, as well as observations of polar bears, sea mammals, and birds. Vast, unique material has been accumulated during this period; the data obtained have considerably changed the current views on the functions of marine Arctic ecosystems, especially in winter and spring, when observations from ships of non-icebreaker class [1][2][3]. Below, we describe the structural characteristics of pelagic ecosystems of the Barents and Kara Seas in winter and spring.The results of the studies have yielded the pattern of the vertical distribution of hydrological parameters in the sea. We found that distinct gradients of salinity were preserved in zones where sea and estuary waters were mixed even in winter, under a continuous layer of ice. Four main types of waters were distinguished in the aquatic area studied: untransformed and slightly transformed waters of sea origin, intermediate waters of runoff currents, and waters from river-sea barrier zones. The finding of the third type is especially important because substances from the continental runoff are especially intense in such zones.We obtained first data on the quantitative characteristics of bacterioplankton in winter and the ecological factors that determine them. Bacterioplankton associations observed under the conditions of a thick snow and ice cover at temperatures close to the freezing point were characterized as stable communities with complex structure that had a sufficient store of substrates for vital activity.The total biomass of bacterioplankton varied in a range of three orders of magnitude (from 7.8 to 1300 mg/m 3 ), the mean value being 205 mg/m 3 . These values are an order of magnitude higher than those obtained in the same season of earlier years, mainly in the halistic region of the Arctic Ocean and in the Canadian Arctic [4]. Note that the data on the total bacterioplankton biomass in winter are very few, and there are no data at all on some morphological groups.The total amount of bacteria, as estimated by direct count under an epifluorescent microscope, varied from 9.6 × 10 3 to 935 × 10 3 cells/ml (on average, 240 × 10 3 cells/ml). The surface area of bacterioplankton cells (which is the most adequate measure of these organisms) varied from 35 to 3700 mm 2 /l (on average, 1100 mm 2 /l).The spatial distribution of the numbers and biomass of bacterioplankton in winter had the following main characteristics. These parameters were increased in the zones where waters were mixed. These are, first, zones where the continental and freshwater runoffs interact (the Ob'-Yenisei estuary region and the region of the spread of the White Sea runoff current in the southeastern Barents Sea nea...

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Ecological Factors of Hydrolytic Enzyme Activities in the Ob–Yenisey Estuarine Coastal Waters in Winter and Their Modelling

Cited by 6 publications

References 5 publications

Multidisciplinary ecological assessment of the Alcantara River (Sicily, Italy) using bioindicators

Multidisciplinary ecological assessment of the Alcantara River (Sicily, Italy) using bioindicators

Variation in Ecological Status of Norwegian Sea Water Determined from Hydrolytic Enzyme Activities

Functioning of the Pelagic Ecosystems of Aquatic Areas Covered with Ice in the Barents and Kara Seas in Winter and Spring

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