Ecological diversity of sediment rhizobacteria associated with Phragmites australis along a drainage canal in the Yellow River watershed

Borruso, Luigimaria; Esposito, Alfonso; Bani, Aïda; Ciccazzo, Sonia; Papa, Marianna; Zerbe, Stefan; Brusetti, Lorenzo

doi:10.1007/s11368-016-1498-y

“…Between 18 and 90% of the produced CH 4 in the root zone of emergent macrophytes in wetlands is consumed by aerobic methanotrophs (Grunfeld and Brix 1999;Laanbroek 2010). So far, many studies have focused on microorganisms in their rhizosphere sediment, including methylotroph-and heterotrophmediated processes of carbon and other element cycles (Borruso et al 2017). Through a 16S rRNA gene Illumina MiSeq sequencing, Pietrangelo et al (2018) reported the bacterial community structure on the root surface of P. australis was indeed different from that of T. latifolia.…”

Section: Introductionmentioning

confidence: 99%

Diversity of active root-associated methanotrophs of three emergent plants in a eutrophic wetland in northern China

Cui

¹

,

Zhao

²

,

Wang

³

et al. 2020

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Root-associated aerobic methanotrophs play an important role in regulating methane emissions from the wetlands. However, the influences of the plant genotype on root-associated methanotrophic structures, especially on active flora, remain poorly understood. Transcription of the pmoA gene, encoding particulate methane monooxygenase in methanotrophs, was analyzed by reverse transcription PCR (RT-PCR) of mRNA isolated from root samples of three emergent macrophytes, including Phragmites australis, Typha angustifolia, and Schoenoplectus triqueter (syn. Scirpus triqueter L.) from a eutrophic wetland. High-throughput sequencing of pmoA based on DNA and cDNA was used to analyze the methanotrophic community. Sequencing of cDNA pmoA amplicons confirmed that the structure of active methanotrophic was not always consistent with DNA. A type I methanotroph, Methylomonas, was the most active group in P. australis, whereas Methylocystis, a type II methanotroph, was the dominant group in S. triqueter. In T. angustifolia, these two types of methanotroph existed in similar proportions. However, at the DNA level, Methylomonas was predominant in the roots of all three plants. In addition, vegetation type could have a profound impact on root-associated methanotrophic community at both DNA and cDNA levels. These results indicate that members of the genera Methylomonas (type I) and Methylocystis (type II) can significantly contribute to aerobic methane oxidation in a eutrophic wetland.

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“…This lake is the largest freshwater lake in the Yellow River watershed; it is a rare, large-scale, shallow lake and grass wetland in this arid region. It has important ecological functions that maintain water resources, regulate the floods and droughts associated with an arid climate, and provide high biological diversity as a Ramsar site (Borruso et al, 2017). The lake recently became eutrophic after receiving large volumes of irrigation drainage water as well as municipal and industrial wastewater with high nitrogen and phosphorus contents from the Hetao Basin (Wu et al, 2017).…”

mentioning

confidence: 99%

“…Phragmites australis (common reed), Typha angustifolia (narrow leaf cattail), and Scirpus triqueter (bulrush) are the dominant macrophytes of WLSH (Duan et al, 2005). Many studies have examined microorganisms in their rhizosphere sediments, including the methylotroph-and heterotrophmediated processes of carbon and other element cycles (Borruso et al, 2017). However, few studies have focused on the characteristics of methanotrophic communities in the roots of macrophytes or on denitrification by aerobic methanotrophs themselves in natural wetlands.…”

mentioning

confidence: 99%

Enrichment of Type I Methanotrophs with <i>nirS</i> Genes of Three Emergent Macrophytes in a Eutrophic Wetland in China

Liu

¹

,

Bao

²

,

Cao

³

et al. 2020

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The pmoA gene, encoding particulate methane monooxygenase in methanotrophs, and nirS and nirK genes, encoding bacterial nitrite reductases, were examined in the root and rhizosphere sediment of three common emergent macrophytes (Phragmites australis, Typha angustifolia, and Scirpus triqueter) and unvegetated sediment from eutrophic Wuliangsuhai Lake in China. Sequencing analyses indicated that 334 out of 351 cloned pmoA sequences were phylogenetically the most closely related to type I methanotrophs (Gammaproteobacteria), and Methylomonas denitrificans-like organisms accounted for 44.4% of the total community. In addition, 244 out of 250 cloned nirS gene sequences belonged to type I methanotrophs, and 31.2% of nirS genes were the most closely related to paddy rice soil clone SP-2-12 in Methylomonas of the total community. Three genera of type I methanotrophs, Methylomonas, Methylobacter, and Methylovulum, were common in both pmoA and nirS clone libraries in each sample. A quantitative PCR (qPCR) analysis demonstrated that the copy numbers of the nirS and nirK genes were significantly higher in rhizosphere sediments than in unvegetated sediments in P. australis and T. angustifolia plants. In the same sample, the nirS gene copy number was significantly higher than that of nirK. Furthermore, type I methanotrophs were localized in the root tissues according to catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH). Thus, nirS-carrying type I methanotrophs were enriched in macrophyte root and rhizosphere sediment and are expected to play important roles in carbon/nitrogen cycles in a eutrophic wetland.

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“…Research has shown that type I methanotrophs are always found in soils with a limited CH 4 supply because they grow better than type II methanotrophs in a low-CH 4 . These results support that members of type I and type II methanotrophs inhabiting in aquatic plants in wetland.…”

Section: Discussionmentioning

confidence: 99%

Diversity of active root-associated methanotrophs of three emergent plants in a eutrophic wetland in northern China

Cui

¹

,

Zhao

²

,

Wang

³

et al. 2020

Preprint

0

View full text Add to dashboard Cite

Root-associated aerobic methanotrophs play an important role in regulating methane emissions from the wetlands. However, the influences of the plant genotype on root-associated methanotrophic structures, especially on active flora, remain poorly understood. Transcription of the pmoA gene, encoding particulate methane monooxygenase in methanotrophs, was analyzed by reverse transcription PCR (RT-PCR) of mRNA isolated from root samples of three emergent macrophytes, including Phragmites australis, Typha angustifolia, and Schoenoplectus triqueter (syn. Scirpus triqueter L.) from a eutrophic wetland. High-throughput sequencing of pmoA based on DNA and cDNA was used to analyze the methanotrophic community. Sequencing of cDNA pmoA amplicons confirmed that the structure of active methanotrophic was not always consistent with DNA. A type I methanotroph, Methylomonas, was the most active group in P. australis, whereas Methylocystis, a type II methanotroph, was the dominant group in S. triqueter. In T. angustifolia, these two types of methanotroph existed in similar proportions. However, at the DNA level, Methylomonas was predominant in the roots of all three plants. In addition, vegetation type could have a profound impact on root-associated methanotrophic community at both DNA and cDNA levels. These results indicate that members of the genera Methylomonas (type I) and Methylocystis (type II) can significantly contribute to aerobic methane oxidation in a eutrophic wetland.Key points1. Root-associated Methylomonas was predominant in three macrophytes using DNA approach. 2. Active Methylocystis was dominant in genera Typha and Schoenoplectus, but not in Phragmites. 3. Plant species impact on methanotrophic communities in both DNA and cDNA levels.

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Ecological diversity of sediment rhizobacteria associated with Phragmites australis along a drainage canal in the Yellow River watershed

Cited by 19 publications

References 41 publications

Diversity of active root-associated methanotrophs of three emergent plants in a eutrophic wetland in northern China

Diversity of active root-associated methanotrophs of three emergent plants in a eutrophic wetland in northern China

Enrichment of Type I Methanotrophs with <i>nirS</i> Genes of Three Emergent Macrophytes in a Eutrophic Wetland in China

Diversity of active root-associated methanotrophs of three emergent plants in a eutrophic wetland in northern China

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