Phytoplankton studies in the Betania Dam (Huila, Colombia) are scarce, there are only three published articles about it, and only one focused on the structure of the community, published 30 years ago, reporting 10 classes, 55 genera and 88 phytoplankton species as well as a deterioration in the water quality due to the contribution of fertilizers and biocides to the Dam. Likewise, the geomorphological characteristics of the Dam contributed to eutrophication, which needs to be focused on since Betania Dam is a very important source of economic income for the region, due to the generation of hydroelectric energy, traditional fishing, fish farming, tourism, and sports. Our objective was to analyze the composition of the current phytoplankton community in Betania Dam, highlighting the differences regards the initial filling conditions. We collected water samples bimonthly in 10 sampling stations distributed throughout the Dam over