Ecological characterization of<i>Aedes aegypti</i>larval habitats (Diptera: Culicidae) in artificial water containers in Girardot, Colombia

García-Sánchez, Diana; Agudelo, Gabriel Antonio Pinilla; Quintero, Juliana

doi:10.1111/jvec.12269

Cited by 34 publications

(23 citation statements)

References 38 publications

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“…An appropriate aquatic habitat for A. aegypti is regulated by biotic and abiotic factors and their interactions. As expected, the temperature and pH were generally around 29°C and 7.5, respectively, regardless of the type of container and the territory, as they have been shown to be favourable for larval development and survival [51][52][53]. Dissolved oxygen, electrical conductivity, salinity, COD, turbidity and the concentrations of Ca, Mg, Fe, Zn and Cu depended on the territory and/ or the container type, the highest values being associated with tyres and plant containers.…”

Section: Discussionmentioning

confidence: 78%

Natural Variation in Physicochemical Profiles and Bacterial Communities Associated with Aedes aegypti Breeding Sites and Larvae on Guadeloupe and French Guiana

et al. 2020

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Aedes aegypti develop in aquatic habitats in which mosquito larvae are exposed to physicochemical elements and microorganisms that may influence their life cycle and their ability to transmit arboviruses. Little is known about the natural bacterial communities associated with A. aegypti or their relation to the biotic and abiotic characteristics of their aquatic habitats. We characterized the physicochemical properties and bacterial microbiota of A. aegypti breeding sites and larvae on Guadeloupe and in French Guiana. In addition, we explored whether geographic location, the type of breeding site and physicochemical parameters influenced the microbiota associated with this mosquito species. We used large-scale 16S rRNA gene sequencing of 160 breeding sites and 147 pools of A. aegypti larvae and recorded 12 physicochemical parameters at the sampled breeding sites. Ordination plots and multiple linear regression were used to assess the influence of environmental factors on the bacterial microbiota of water and larvae. We found territory-specific differences in physicochemical properties (dissolved oxygen, conductivity) and the composition of bacterial communities in A. aegypti breeding sites that influenced the relative abundance of several bacteria genera (e.g., Methylobacterium, Roseoccocus) on the corresponding larvae. A significant fraction of the bacterial Electronic supplementary material The online version of this article (

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Section: Discussionmentioning

confidence: 78%

Natural Variation in Physicochemical Profiles and Bacterial Communities Associated with Aedes aegypti Breeding Sites and Larvae on Guadeloupe and French Guiana

et al. 2020

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“…aegypti is regulated by biotic and abiotic factors and their interactions. As expected, the temperature and pH were generally around 29 °C and 7.5, respectively, regardless of the type of container and the territory, as they have been shown to be favourable for larval development and survival [47,48,49]. Dissolved oxygen, electrical conductivity, salinity, COD, turbidity and the concentrations of Ca, Mg, Fe, Zn and Cu depended on the territory and/or the container type, the highest values being associated with tyres and plant containers.…”

Section: Discussionmentioning

confidence: 67%

Natural variation in physicochemical profiles and bacterial communities associated with Aedes aegypti breeding sites and larvae on Guadeloupe and French Guiana

Héry

Guidez

Durand

et al. 2020

Preprint

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Background: Immature stages of Aedes aegypti develop in many man-made aquatic habitats in which mosquito larvae are exposed to physicochemical elements and microorganisms that may influence their life cycle and their ability to transmit human arboviruses. Despite the omnipresence of Ae. aegypti in tropical and subtropical regions, little is known about the natural bacterial communities associated with this mosquito or their relation to the biotic and abiotic characteristics of their aquatic habitats. We comprehensively characterized the physicochemical properties and bacterial microbiome of Ae. aegypti breeding sites and larvae on Guadeloupe and in French Guiana. In addition, we explored whether geographical location, the type of breeding site and physicochemical parameters of the water influenced the microbiota associated with this mosquito species. Method:We used large-scale 16S rRNA gene sequencing of 160 breeding sites and 147 pools of Ae.aegypti larvae from sites widely distributed across Guadeloupe and French Guiana and recorded 13 physicochemical parameters at the sampled breeding sites. Ordination plots and multiple linear regression were used to assess the influence of environmental factors on the bacterial microbiome of water and larvae. Results:We found territory-specific differences in physicochemical properties (dissolved oxygen, conductivity and metal content) and the composition of bacterial communities in Ae. aegypti breeding sites that influenced the relative abundance of several bacteria genera (Methylobacterium, Roseoccocus) on the corresponding larvae. A significant fraction of the bacterial communities identified on Ae. aegypti larvae, dominated by Herbiconiux and Microvirga genera, were consistently enriched in mosquitoes regardless the location. Conclusion:Territory-specific differences observed in the biotic and abiotic properties of Ae. aegypti breeding sites influenced a fraction of the microbial communities of the corresponding larvae, raising concern about the impact of these changes on pathogen transmission by different Ae. aegypti populations. BackgroundAedes aegypti (Diptera: Culicidae) is a mosquito vector of arboviruses such as yellow fever, dengue, 4 chikungunya and Zika that constitute major global health problems and threaten the French overseas territories of Guadeloupe and French Guiana [1,2]. Besides the high susceptibility for these pathogens, this mosquito is a main vector of arboviruses because it is highly anthropophilic and thrives close to humans in urban and peri-urban areas [3,4]. The species breeds mainly in humanmade containers with volumes ranging from a few millilitres to hectolitres, such as drums, plastic buckets, cisterns, flower vases and rubber tyres [4,5].Water quality is critical for the choice of oviposition site for gravid females to ensure successful, complete development of their progeny from larvae to adults [6,7,8]. Females select breeding sites according to biotic and abiotic elements in the water, such as organic matter [8], bacteria [9, 10], phosp...

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“…As reported by Obi et al (2017), relative abundance of mosquitoes has significantly correlated negatively with those microinvertebrates in rock pools [38]. Garcia-Sánchez et al [39] reported the presence of three main phyla of algae, Bacillariophyceae, Chlorophyceae, and Cyanobacteria in Aedes aegypti larval habitats in artificial water containers in Girardot, Colombia. Similarly, these algal phyla were reported from both natural and man-made breeding habitats in our study.…”

Section: Biomed Research Internationalmentioning

confidence: 62%

Naturally Occurring Microbiota Associated with Mosquito Breeding Habitats and Potential Parasitic Species against Mosquito Larvae: A Study from Gampaha District, Sri Lanka

Ranasinghe

Amarasinghe

2020

BioMed Research International

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A mosquito species has its own favourable requirements of abiotic and biotic characteristics including microbiota, in a breeding habitat. Some of the microbiota may cause parasitic or pathogenic effects to mosquito larvae such as species of viruses, parasitic bacteria, fungi, protists, entomopathogenic nematodes, and filamentous fungi. In Sri Lanka, there is a scarcity of information on microbiota associated with mosquito breeding habitats and their effect on mosquito larvae. Hence, the present study was conducted to determine microbiota species/taxa associated with a variety of mosquito breeding habitats in selected areas of the Gampaha District in Sri Lanka and the relationship, if any, the microbiota has with mosquito larva survival and breeding. Forty-five microbiota species belonging to 11 phyla were found from different mosquito breeding habitats with the highest percentage belonging to phylum Euglenozoa (27.89%). Species that belonged to the phylum Amoebozoa (1.22%) and Sarcodina (1.17%) had the lowest abundance, and each of its species richness was recorded as one. Philodina citrina followed by Monostyla bulla comprised 30.8% and 16.59%, respectively, of the total rotifer population. From the total microbiota, 25-50% existed as accidental while less than 25% rare, in the habitat type according to their abundance. Paddy fields had the highest species richness (17), evenness (23.52), Shannon-Weiner (66.64), and beta diversity (0.65) over 50% indicating high heterogeneity in microbiota composition among the habitats. Ciliated protists, namely, Vorticella microstoma, Zoothamnium spp., and Chilodinella sp., were identified as naturally occurring microbiota associated with Culex mosquito larvae that inhabited in paddy fields and associated irrigation canals. Only Vorticella microstoma caused a significant lethal effect on mosquito larvae. This study revealed that species of Cx. gelidus, Cx. pseudovishnui, Cx. tritaeniorhynchus, Cx. quinquefasciatus, and Cx. whitmorei served as hosts for V. microstoma where infectivity rate in Cx. tritaeniorhynchus reached 73.22. Chilodinella sp. selectively served as endoparasitic to Cx. gelidus larvae causing only 4.58% mortality, and invasive cysts of the pathogen were observed in the subcuticular layer of the host body. Even though Zoothamnium spp. were found on Cx. tritaeniorhynchus larvae, there was no lethal effect due to the attachment of the parasitic agent. The potential of these microbiotas in integrated vector controlling approaches in future perspectives is recommended.

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Ecological characterization ofAedes aegyptilarval habitats (Diptera: Culicidae) in artificial water containers in Girardot, Colombia

Cited by 34 publications

References 38 publications

Natural Variation in Physicochemical Profiles and Bacterial Communities Associated with Aedes aegypti Breeding Sites and Larvae on Guadeloupe and French Guiana

Natural Variation in Physicochemical Profiles and Bacterial Communities Associated with Aedes aegypti Breeding Sites and Larvae on Guadeloupe and French Guiana

Natural variation in physicochemical profiles and bacterial communities associated with Aedes aegypti breeding sites and larvae on Guadeloupe and French Guiana

Naturally Occurring Microbiota Associated with Mosquito Breeding Habitats and Potential Parasitic Species against Mosquito Larvae: A Study from Gampaha District, Sri Lanka

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