Ecological and Agricultural Significance of Bacterial Polyhydroxyalkanoates

Kadouri, Daniel E.; Jurkevitch, Édouard; Okon, Yaacov; Castro‐Sowinski, Susana

doi:10.1080/10408410590899228

Cited by 230 publications

(131 citation statements)

References 79 publications

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“…In support of this, oceanic DOC concentrations tend to be highest in the low nutrient oligotrophic gyres (Hansell et al, 2009). Even in eutrophic waters, as long as the C / N ratio reaches a threshold value, microbial cells will start to store carbon and produce more recalcitrant compounds or polymers (Bhaskar and Bhosle, 2005;Kadouri et al, 2005;Jiao et al, 2010b). In a 14-day in situ nutrient enrichment experiment undertaken in the western Pacific oligotrophic gyre, more than 30 % of the ambient organic carbon was respired in the incubation with addition of inorganic nutrients compared to the control (Liu et al, 2014).…”

Section: Nutrient Supplymentioning

confidence: 99%

Mechanisms of microbial carbon sequestration in the ocean – future research directions

Jiao

Robinson²,

Azam³

et al. 2014

Biogeosciences

192

101

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Abstract. This paper reviews progress on understanding biological carbon sequestration in the ocean with special reference to the microbial formation and transformation of recalcitrant dissolved organic carbon (RDOC), the microbial carbon pump (MCP). We propose that RDOC is a concept with a wide continuum of recalcitrance. Most RDOC compounds maintain their levels of recalcitrance only in a specific environmental context (RDOC t ). The ocean RDOC pool also contains compounds that may be inaccessible to microbes due to their extremely low concentration (RDOC c ). This differentiation allows us to appreciate the linkage between microbial source and RDOC composition on a range of temporal and spatial scales.Analyses of biomarkers and isotopic records show intensive MCP processes in the Proterozoic oceans when the MCP could have played a significant role in regulating climate. Understanding the dynamics of the MCP in conjunction with the better constrained biological pump (BP) over geological timescales could help to predict future climate trends. Integration of the MCP and the BP will require new research approaches and opportunities. Major goals include understanding the interactions between particulate organic carbon (POC) and RDOC that contribute to sequestration efficiency, and the concurrent determination of the chemical composition of organic carbon, microbial community composition and enzymatic activity. Molecular biomarkers and isotopic tracers should be employed to link water column processes Published by Copernicus Publications on behalf of the European Geosciences Union. N. Jiao et al.: Mechanisms of microbial carbon sequestration in the oceanto sediment records, as well as to link present-day observations to paleo-evolution. Ecosystem models need to be developed based on empirical relationships derived from bioassay experiments and field investigations in order to predict the dynamics of carbon cycling along the stability continuum of POC and RDOC under potential global change scenarios. We propose that inorganic nutrient input to coastal waters may reduce the capacity for carbon sequestration as RDOC. The nutrient regime enabling maximum carbon storage from combined POC flux and RDOC formation should therefore be sought.

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Section: Nutrient Supplymentioning

confidence: 99%

Mechanisms of microbial carbon sequestration in the ocean – future research directions

Jiao

Robinson²,

Azam³

et al. 2014

Biogeosciences

192

101

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“…Cells with higher PHA content can survive longer than those with lower amounts, and PHA degraded elements can be used rapidly for numerous metabolic needs. Accumulation of PHA can provide the cell with the ability to endure a variety of harmful physical and chemical stresses (Castro-Sowinski et al, 2010;Kadouri et al, 2005).…”

Section: Formulation and Low-cost Are Crucial Aspect Of Producing Inomentioning

confidence: 99%

Legume Crops, Importance and Use of Bacterial Inoculation to Increase Production

Morel¹,

Braña²,

Castro‐Sowinski³

2012

Crop Plant

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“…In the transmissive phase of L. pneumophila, high amounts of cytoplasmic granules of poly-3-hydroxybutyrate (PHB) 4 are observed in L. pneumophila. Generally, this polymer is known as an important energy and carbon storage for some bacteria (1,(5)(6)(7)(8). Indeed, PHB is also essential for the survival of L. pneumophila in the environment where it is catabolized during the viable but non-culturable state of L. pneumophila (7)(8)(9)(10).…”

mentioning

confidence: 99%

“…Generally, this polymer is known as an important energy and carbon storage for some bacteria (1,(5)(6)(7)(8). Indeed, PHB is also essential for the survival of L. pneumophila in the environment where it is catabolized during the viable but non-culturable state of L. pneumophila (7)(8)(9)(10). However, less is known about the temporary amounts of PHB and the dynamics of PHB metabolism during the life cycle of L. pneumophila (1, 7, 10 -12).…”

mentioning

confidence: 99%

Growth-related Metabolism of the Carbon Storage Poly-3-hydroxybutyrate in Legionella pneumophila

Gillmaier

Schunder

Kutzner

et al. 2016

Journal of Biological Chemistry

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Legionella pneumophila, the causative agent of Legionnaires disease, has a biphasic life cycle with a switch from a replicative to a transmissive phenotype. During the replicative phase, the bacteria grow within host cells in Legionella-containing vacuoles. During the transmissive phenotype and the postexponential (PE) growth phase, the pathogens express virulence factors, become flagellated, and leave the Legionella-containing vacuoles. Using 13 C labeling experiments, we now show that, under in vitro conditions, serine is mainly metabolized during the replicative phase for the biosynthesis of some amino acids and for energy generation. During the PE phase, these carbon fluxes are reduced, and glucose also serves as an additional carbon substrate to feed the biosynthesis of poly-3-hydroxybuyrate (PHB), an essential carbon source for transmissive L. pneumophila. Whole-cell FTIR analysis and comparative isotopologue profiling further reveal that a putative 3-ketothiolase (Lpp1788) and a PHB polymerase (Lpp0650), but not enzymes of the crotonylCoA pathway (Lpp0931-0933) are involved in PHB metabolism during the PE phase. However, the data also reflect that additional bypassing reactions for PHB synthesis exist in agreement with in vivo competition assays using Acanthamoeba castellannii or human macrophage-like U937 cells as host cells. The data suggest that substrate usage and PHB metabolism are coordinated during the life cycle of the pathogen.

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Ecological and Agricultural Significance of Bacterial Polyhydroxyalkanoates

Cited by 230 publications

References 79 publications

Mechanisms of microbial carbon sequestration in the ocean – future research directions

Mechanisms of microbial carbon sequestration in the ocean – future research directions

Legume Crops, Importance and Use of Bacterial Inoculation to Increase Production

Growth-related Metabolism of the Carbon Storage Poly-3-hydroxybutyrate in Legionella pneumophila

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